Reverse Phase Protein Arrays: From Technical and Analytical Fundamentals to Applications 1st ed. 2019 [Kietas viršelis]

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  • Formatas: Hardback, 266 pages, aukštis x plotis: 235x155 mm, 50 Tables, color; 57 Illustrations, color; 7 Illustrations, black and white; VIII, 266 p. 64 illus., 57 illus. in color., 1 Hardback
  • Serija: Advances in Experimental Medicine and Biology 1188
  • Išleidimo metai: 21-Jan-2020
  • Leidėjas: Springer Verlag, Singapore
  • ISBN-10: 9813297549
  • ISBN-13: 9789813297548
Kitos knygos pagal šią temą:
  • Formatas: Hardback, 266 pages, aukštis x plotis: 235x155 mm, 50 Tables, color; 57 Illustrations, color; 7 Illustrations, black and white; VIII, 266 p. 64 illus., 57 illus. in color., 1 Hardback
  • Serija: Advances in Experimental Medicine and Biology 1188
  • Išleidimo metai: 21-Jan-2020
  • Leidėjas: Springer Verlag, Singapore
  • ISBN-10: 9813297549
  • ISBN-13: 9789813297548
Kitos knygos pagal šią temą:

This book provides practical guidance on all aspects of reverse phase protein array (RPPA) technology, which permits the quantification of protein levels in cell or tissue lysates. In addition, the latest results are presented from laboratories across the world where experts are successfully running the challenging RPPA data platform. After an introductory chapter by the inventor of the RPPA technique, the demanding task of lysate preparation is explained. Subsequent chapters review printing platforms and analytical platforms and discuss antibody screening in detail. The role of RPPA data in integrative analyses is thoroughly examined, and the various applications of RPPA, for example to identify molecular targeting drugs or evaluate cancer drug efficacy, are discussed with reference to the latest knowledge. It is hoped that the book will foster the implementation of RPPA by documenting the practical details of the technology and by revealing its great potential. It will be of high value for researchers who plan to build an RPPA platform in their institute and for researchers, clinicians, and companies who use RPPA through collaboration with an existing RPPA facility.


Chapter 1: Origin and future of RPPA technology.
Chapter 2: Lysate preparation.
Chapter 3: Printing platforms 1.
Chapter 4: Printing platforms 2.
Chapter 5: Analytical platforms 1.
Chapter 6: Analytical platforms 2.-
Chapter 7: Analytical platforms 3.
Chapter
8. Antibody screening 1.
Chapter
9. Antibody screening 2.
Chapter
10. Antibody screening 3.
Chapter
11. Integrative analysis for genomics and RPPA.
Chapter
12. Integrative analysis of multiple platforms of RPPA.
Chapter
13. Drug screening platforms and RPPA.
Chapter
14. RPPA in small population analysis.
Chapter 15: Molecular target identification using RPPA.
Chapter 16: RPPA in clinical trials.-
Chapter 17: RPPA and future medicine.
Tesshi Yamada, M.D., Ph.D. Professor of International University of Health and Welfare, Narita, Japan Satoshi Nishizuka, M.D., Ph.D. Professor of Iwate Medical University, Japan Gordon Mills, M.D., Ph.D. Professor of University of Texas, MD Anderson Cancer Center Lance Liotta, M.D., Ph.D. Co-Director of the Center for Applied Proteomics and Molecular Medicine, George Mason University