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El. knyga: Acute Myeloid Leukemia: Methods and Protocols

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  • Formatas: EPUB+DRM
  • Serija: Methods in Molecular Biology 1633
  • Išleidimo metai: 22-Jul-2017
  • Leidėjas: Humana Press Inc.
  • Kalba: eng
  • ISBN-13: 9781493971428
Kitos knygos pagal šią temą:
  • Formatas: EPUB+DRM
  • Serija: Methods in Molecular Biology 1633
  • Išleidimo metai: 22-Jul-2017
  • Leidėjas: Humana Press Inc.
  • Kalba: eng
  • ISBN-13: 9781493971428
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This volume describes up-to-date techniques used in understanding the molecular biology of acute myeloid leukemia (AML) and addressing advances in diagnosis, classification, prognostication, and therapeutic strategies to potentially impact overall patient survival. The chapters in this book cover topics such as: cytochemical staining, single-cell mass cytometry of AML and Leukemia stem/progenitor cells, microsphere-based assessment of DNA methylation for AML prognosis, a zebrafish model for evaluating the function of human leukemic gene IDH1 and its mutation, and the isolation of biologically active exosomes from plasma of patients with cancer. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.


Cutting-edge and practical, Acute Myeloid Leukemia: Methods and Protocols is a valuable resource for scientists and researchers to further their studies and advancements in the field of AML.

1 Molecular Malfeasance Mediating Myeloid Malignancies: The Genetics of Acute Myeloid Leukemia
1(18)
Rebecca L. King
Adam Bagg
2 Cytochemical Staining
19(14)
Michele E. Paessler
Marybetb Helfrich
Gerald B.W. Wertheim
3 Immunohistochemistry in Acute Myeloid Leukemia
33(18)
Michael W. Cruise
4 Immunophenotyping by Multiparameter Flow Cytometry
51(24)
Weina Chen
Hung S. Luu
5 Single-Cell Mass Cytometry of Acute Myeloid Leukemia and Leukemia Stem/Progenitor Cells
75(12)
Zhihong Zeng
Marina Konopleva
Michael Andreeff
6 Design and Application of Multiplex PCR Seq for the Detection of Somatic Mutations Associated with Myeloid Malignancies
87(14)
Naomi Park
George Vassiliou
7 AMLprofiler: A Diagnostic and Prognostic Microarray for Acute Myeloid Leukemia
101(24)
Marco Alessandrini
Sharon S. Kappala
Michael S. Pepper
8 Microsphere-Based Assessment of DNA Methylation for AML Prognosis
125(12)
Gerald B. W. Wertheim
Marlise R. Luskin
Martin Carroll
Stephen R. Master
9 Genome-Wide Analysis of DNA Methylation in Hematopoietic Cells: DNA Methylation Analysis by WGBS
137(14)
Mira Jeong
Anna G. Guzman
Margaret A. Goodell
10 Detection and Quantification of Acute Myeloid Leukemia-Associated Fusion Transcripts
151(12)
Jonathan Schumacher
Philippe Szankasi
Todd W Kelley
11 Targeted Next-Generation Sequencing of Acute Leukemia
163(22)
Eric Konnick
Christina M. Lockwood
David Wu
12 Bioinformatics Data Analysis of Next-Generation Sequencing Data from Heterogeneous Tumor Samples
185(8)
Sean R. Landman
Toe Hyun Hwang
13 A Zebrafish Model for Evaluating the Function of Human Leukemic Gene IDH1 and Its Mutation
193(26)
Alvin C.H. Ma
Xiangguo Shi
Bai-Liang He
Yuhan Guo
Anskar T.H. Leung
14 Measurement of Oncometabolites D-2-Hydroxyglutaric Acid and L-2-Hydroxyglutaric Acid
219(16)
Patricia M. Jones
Richard Boriack
Eduard A. Struys
Dinesh Rakheja
15 Nanofluidic Allele-Specific Digital PCR Method for Quantifying IDH1 and IDH2 Mutation Burden in Acute Myeloid Leukemia
235(22)
Daniel H. Wiseman
Tim C.P. Somervaille
16 Isolation of Biologically Active Exosomes from Plasma of Patients with Cancer
257(10)
Chang-Sook Hong
Sonja Funk
Theresa L. Whiteside
17 Generating and Expanding Autologous Chimeric Antigen Receptor T Cells from Patients with Acute Myeloid Leukemia
267(10)
Saad S. Kenderian
Carl H. June
Saar Gill
Index 277