| Preface |
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v | |
| Contents |
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vii | |
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List of Participants of European Projects |
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xvii | |
| Abbreviations |
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xxi | |
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Induction of Molluscan Cytochrome P450 Monooxygenase System as a Biomarker of Organic Pollution in Environmental Monitoring |
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1 | (28) |
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The uptake of xenobiotics and bioaccumulation in aquatic molluscs |
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2 | (1) |
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Mixed function oxidase (MFO)-dependent metabolism |
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2 | (1) |
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CYP1A as a biomonitoric tool |
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3 | (1) |
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The molluscan MFO system: physical and catalytic properties |
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4 | (2) |
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Multiple CYP forms in Mytilus sp |
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6 | (1) |
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Regulation of CYP forms in Mytilus sp |
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7 | (2) |
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Xenobiotic-dependent changes in Mytilus sp. MFO properties: laboratory studies |
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9 | (5) |
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Putative induction of the CYP1-immunopositive protein |
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11 | (3) |
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Xenobiotic-dependent changes in Mytilus sp. MFO properties: field studies |
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14 | (8) |
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Responses of M. galloprovincialis CYP-immunopositive proteins in The Venice Lagoon, Italy |
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15 | (3) |
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Correlations between digestive gland microsomal CYP1A-immunopositive protein and BPH activity on Mytilus sp.: recent studies |
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18 | (4) |
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22 | (7) |
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22 | (7) |
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Sensitivity and Specificity of Metallothionein as a Biomarker for Aquatic Environment Biomonitoring |
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29 | (16) |
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30 | (1) |
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31 | (1) |
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32 | (13) |
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39 | (6) |
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Genotoxicity Biomarkers in Aquatic Organisms as Indictors of Carciaogenlc Marine Pollutants |
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45 | (20) |
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46 | (2) |
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47 | (1) |
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48 | (1) |
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48 | (3) |
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51 | (7) |
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58 | (7) |
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60 | (5) |
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DNA Adduct Detection in Mussels Exposed to Bulky Aromatic Compounds in Laboratory and Field Conditions |
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65 | (20) |
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66 | (2) |
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Biological relevance of DNA adducts |
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66 | (1) |
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The 32P-postlabelling assay |
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67 | (1) |
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DNA adduct detection and environmental monitoring |
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67 | (1) |
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Formation and detection of DNA adducts in mussels |
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68 | (9) |
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DNA adducts in mussels following laboratory exposure to genotoxins |
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69 | (1) |
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DNA adducts in mussels following field exposure |
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70 | (7) |
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77 | (8) |
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78 | (7) |
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Developmental, Cytogenetic and Biochemical Effects of Spiked or Environmentally Polluted Sediments in Sea Urchin Bioassays |
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85 | (46) |
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87 | (1) |
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88 | (3) |
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91 | (40) |
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120 | (2) |
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122 | (6) |
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128 | (3) |
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Comparative Study of Sediment and Mussel Aromatic Compound Content in European Coastal Environments. Relationship with Specific Biomarkers |
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131 | (48) |
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133 | (3) |
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Sediment PAH contamination |
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136 | (14) |
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136 | (1) |
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Sediment total PAH concentration |
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137 | (1) |
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Origin of the sediment contamination |
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138 | (6) |
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Relationship between the origin and the extent of the sediment Contamination |
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144 | (2) |
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Relationship between sediment PAH contamination and sediment organic matter content |
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146 | (1) |
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Grain-size PAH distribution |
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147 | (3) |
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150 | (16) |
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151 | (1) |
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Mussel total PAH concentration |
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151 | (1) |
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Relation between mussel and sediment total PAH concentration |
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152 | (3) |
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Exposure of mussels to PAH contaminants present in the water column |
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155 | (11) |
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Impact of sediment contamination on organisms |
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166 | (13) |
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MFO activity in relation with sediment PAH contamination |
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166 | (3) |
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Selective biotransformation capacity: case study of two isomeric compounds, benzo[ a]pyrene and benzo [ e]pyrene |
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169 | (3) |
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172 | (1) |
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173 | (6) |
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Monitoring of Biological Effects of Pollutants: Field Application |
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179 | (36) |
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Monitoring biological effects along French coasts |
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181 | (8) |
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181 | (2) |
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EROD application in two Pilot sites |
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183 | (6) |
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189 | (1) |
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Crassostrea Gigas Embryo and Larval Bioassays in Waters and Sediments |
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189 | (7) |
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189 | (2) |
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Fresh and brackish water biomonitoring |
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191 | (2) |
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Sediment biomonitoring with C. gigas embryos and larvae |
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193 | (3) |
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196 | (1) |
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Testing biomarkers at sea: an in situ approach in the Northwestern Mediterranean Sea |
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196 | (7) |
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196 | (1) |
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Three biomarkers of exposure along the Mediterranean Sea |
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196 | (5) |
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201 | (2) |
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Molecular epidemiology studies using ras oncogene as a genetic marker of malignancy for dragonet callionymus lyra along the French coast. The Seine estuary |
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203 | (3) |
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203 | (1) |
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Dragonet exposure to PCBs and measurements of DNA adducts |
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204 | (1) |
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Ras gene mutation analysis |
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205 | (1) |
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206 | (1) |
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206 | (9) |
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209 | (6) |
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Biochemical Markers in Mussel, Mytilus sp., and Pollution Monitoring in European Coasts: Data Analysis |
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215 | (22) |
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216 | (1) |
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217 | (20) |
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Sample collection and preparation |
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217 | (1) |
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217 | (1) |
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217 | (1) |
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218 | (16) |
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234 | (3) |
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Investigation of Genotoxicity and Immunotoxicity for Monitoring Marine Pollution in the Baltic Sea and Mediterranean Sea |
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237 | (22) |
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239 | (1) |
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240 | (4) |
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244 | (9) |
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253 | (6) |
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255 | (4) |
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Biochemical Tools for the Assessment of Pesticide Exposure in a Deltaic Environment: The Use of Cholinesterases and Carboxylesterases |
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259 | (20) |
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The Ebro delta: environmental threats |
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261 | (1) |
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Toxicity of organophosphorus pesticides: inhibition of cholinesterases |
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262 | (1) |
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The bivalve farms: Mytilus galloprovincialis |
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263 | (16) |
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Characterisation of esterase activity |
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264 | (1) |
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In vitro effects of pesticides |
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265 | (1) |
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In viva effects of pesticides |
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266 | (1) |
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Seasonal variation in OPs pesticides body burden and esterase activity: a field study |
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267 | (3) |
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Primary cell cultures: a valuable tool to evaluate toxicity and metabolism of pesticides |
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270 | (1) |
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Acetylcholinesterase reactivation techniques |
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271 | (3) |
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Acetylcholinesterase activity in field collected specimens |
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274 | (1) |
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275 | (4) |
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Environmental Monitoring in the North Sea by Combining Biomarker Studies in the Sea Star Asterias Rubens with Sediment Quality Assessment Based on Sea Urchin Bioassays |
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279 | (52) |
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281 | (4) |
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Use of echinoderm species in environmental monitoring |
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281 | (1) |
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Previous research on impairment of reproduction by contaminants and biomarker responses m the sea star A. rubens |
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282 | (2) |
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284 | (1) |
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Scope of the present research |
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284 | (1) |
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285 | (8) |
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285 | (2) |
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Estimating reproductive success of sea starts |
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287 | (1) |
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287 | (2) |
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Contaminant analysis in sediment and sea star tissues |
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289 | (2) |
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291 | (1) |
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292 | (1) |
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293 | (17) |
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293 | (1) |
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Contaminant levels in sea stars (both field trips) |
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293 | (3) |
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Relation between contaminant levels in sea starts and sediment |
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296 | (1) |
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Measurements of reproductive success of sea stars (only first field trip) |
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297 | (1) |
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Biomarker responses in sea starts |
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298 | (4) |
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Sediment contamination and assessment of sediment quality with sea urchin bioassays |
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302 | (8) |
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310 | (21) |
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319 | (7) |
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326 | (5) |
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Cholinesterase Activity as a Bioindicator for Monitoring Marine Pollution in the Baltic Sea and the Mediterranean Sea |
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331 | (12) |
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333 | (1) |
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333 | (2) |
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335 | (4) |
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339 | (4) |
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340 | (3) |
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Evaluation of Various Biomarkers in the Wild Fish Serranus Cabrilla Collected in the NW Mediterranean Sea |
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343 | (14) |
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345 | (1) |
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346 | (2) |
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346 | (1) |
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Sample preparation for enzyme activities |
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347 | (1) |
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Sample preparation for metal measurements |
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347 | (1) |
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348 | (9) |
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355 | (2) |
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Microbiological Indicators for Monitoring Marine Pollution in the Baltic Sea and the Mediterranean Sea |
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357 | (10) |
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358 | (1) |
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358 | (2) |
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360 | (3) |
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363 | (4) |
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365 | (2) |
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Isolation of Cytochrome P450 CDNAS (CYP1A1 and CYP4T2) from the Sea Bass (Dicentrarchus Labrax) Tools for Biomonitoring Sea Pollution |
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367 | (26) |
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369 | (1) |
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370 | (23) |
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Molecular cloning of inducible CYP genes |
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370 | (10) |
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Expression of CYP1A1 and CYP4T2 in untreated sea bass tissues |
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380 | (3) |
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Induction of CYP1A1 and CYP4T2 by xenobiotics in sea bass tissues |
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383 | (7) |
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390 | (3) |
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Inhibitory Effects of Heavy Metals on CYP1A1 Induction in Black Seabream (Spondyliosoma Cantharus) Hepatocyte Cultures |
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393 | (16) |
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395 | (1) |
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396 | (2) |
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398 | (11) |
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406 | (3) |
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Biochemical Responses of Crabs (Carcinus spp) to Polycyclic Aromatic Hydrocarbons (PAHs) as the Basis for New Biomarker Assays |
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409 | (22) |
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410 | (2) |
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412 | (4) |
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Screening for induction of proteins |
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413 | (1) |
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Evidence for the induction of cytochrome P450 |
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414 | (2) |
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416 | (6) |
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416 | (1) |
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DNA fingerprinting using the PCR |
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417 | (1) |
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DNA fingerprinting by AP-PCR as an alternative biomarker assay |
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418 | (3) |
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Other assays for genotoxicity in crabs |
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421 | (1) |
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Linked biomarker responses |
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422 | (1) |
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423 | (8) |
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426 | (5) |
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Cloning of Metallothionein cDNAS in Carcinus Maenas |
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431 | (8) |
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432 | (1) |
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433 | (1) |
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433 | (3) |
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436 | (3) |
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437 | (2) |
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Development of Cytochrome P450 Biomarkers from Posidinia Oceanica |
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439 | (8) |
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440 | (1) |
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441 | (2) |
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443 | (1) |
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444 | (1) |
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445 | (2) |
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445 | (2) |
| Technical Annexes |
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447 | (96) |
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Measurement of Total Cytochrome P450 Content in Digestive Gland Microsomes of Mussel (Mytilus sp.) |
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449 | (2) |
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Measurement of Cytochrome P450 Immunopositive Proteins in Digestive Gland Microsomes of Mussel (Mytilus sp.) by Western Analysis |
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451 | (2) |
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453 | (2) |
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Evaluation of Lysosomal Membrane Stability |
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455 | (6) |
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Alkaline Filter Elution Method |
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461 | (6) |
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467 | (4) |
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Detection of Bulky Aromatic DNA Adducts by 32P-postlabelling |
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471 | (4) |
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Embryotoxicity on Sea Urchins |
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475 | (2) |
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Sediment Spiking Technique |
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477 | (1) |
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Measurement of Oxidative Activity: Luminol-dependeut Chemiluminescence and 8-Oxodesoxyguauine |
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478 | (3) |
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Chemical Analyses of PAH in Sediment and Mussels |
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481 | (6) |
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Procedures for Cholinesterase Determination in Fish and Mussel |
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487 | (4) |
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Microplate Method for Measurement of EthoxyResorufin-O-Deethylase (EROD) in Fish |
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491 | (4) |
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Benzo(a)pyrene Hydroxylase Activity Measurement in Microsomes from Mytilus sp |
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495 | (4) |
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Determination of Cholinesterase Activity in Mussel Gills |
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499 | (2) |
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Chemical Analysis: PAHs, PCBs and Pesticides |
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501 | (2) |
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Biochemical Markers Acetylcholinesterase Activity and Cytochrome P450 |
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503 | (2) |
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Measurement of DNA-Strandbreaks in Tissue of the Sea Star Asterias Rubens |
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505 | (4) |
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Measurement of Sediment-Toxicity Using a Fertilization and Embryonic Development Assay with the Sea Urchins (Psammechinus Miliaris, Paracentrotus Lividus) |
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509 | (4) |
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Determination of EROD, GST and AchE Activities in Painted Comber (Serranus Cabrilla) |
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513 | (4) |
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Miniaturized DNA Unwinding Assay Employing Hydroxyapatite ``Batch'' Elution |
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517 | (2) |
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Molecular Cloning of CYP Genes in the Sea Bass (Dicentrarchus Labrax): Experimental Procedures |
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519 | (8) |
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527 | (2) |
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Determination of Cytochromes P450 Expression and Induction in Black Seabream (Spondyliosama cantharus): Enzyme Activities, Northern and Esters Blots |
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529 | (4) |
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Biochemical Responses of Crabs (Carcinus spp) to Polycyclic Aromatic Hydrocarbons (PAHs) as the Basis for New Biomarkers Assays |
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533 | (4) |
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Cloning of Metallothionein cDNAs in Carcinus Maenas |
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537 | (2) |
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Development of Biomarkers from Seagrass Posidonia Oceanica |
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539 | (4) |
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| List of Contributors |
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543 | (6) |
| Index of Keywords |
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549 | |
Daugiau apie elektronines knygas