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El. knyga: Cancer Stem Cells: Methods and Protocols

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  • Formatas: PDF+DRM
  • Serija: Methods in Molecular Biology 1692
  • Išleidimo metai: 05-Oct-2017
  • Leidėjas: Humana Press Inc.
  • Kalba: eng
  • ISBN-13: 9781493974016
Kitos knygos pagal šią temą:
Cancer Stem Cells: Methods and ProtocolsDidesnis paveikslėlis
  • Formatas: PDF+DRM
  • Serija: Methods in Molecular Biology 1692
  • Išleidimo metai: 05-Oct-2017
  • Leidėjas: Humana Press Inc.
  • Kalba: eng
  • ISBN-13: 9781493974016
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This detailed volume gathers a comprehensive collection of methods, protocols, and procedures used for the identification, characterization, and selection of cancer stem cells. These include surface marker expression, side population, spheres formation, ALDH activity, and numerous others. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and expert tips and troubleshooting advice to avoid potential pitfalls.

Comprehensive and practical, Cancer Stem Cells: Methods and Protocols provides researchers with the techniques they will need in order to pursue vital research into this complex field of study.
Preface v
Contributors ix
1 Introduction to Cancer Stem Cells: Past, Present, and Future
1(8)
David Bakhshinyan
Ashley A. Adile
Maleeha A. Qazi
Mohini Singh
Michelle M. Kameda-Smith
Nick Yelle
Chirayu Chokshi
Chitra Venugopal
Shiela K. Singh
2 Surface Markers for the Identification of Cancer Stem Cells
9(22)
Vinod Gopalan
Farhadul Islam
Alfred King-yin Lam
3 The Role of CD44 and Cancer Stem Cells
31(12)
Liang Wang
Xiangsheng Zuo
Keping Xie
Daoyan Wei
4 Evaluation and Isolation of Cancer Stem Cells Using ALDH Activity Assay
43(6)
Luigi Mele
Davide Liccardo
Virginia Tirino
5 Isolation of Cancer Stem Cells by Side Population Method
49(28)
Masayuki Shimoda
Masahide Ota
Yasunori Okada
6 Self-Renewal and CSCs In Vitro Enrichment: Growth as Floating Spheres
Pooja Mehta
Caymen Novak
Shreya Raghavan
Maria Ward
Geeta Mehta
7 In Vitro Tumorigenic Assay: The Tumor Spheres Assay
77(12)
Hui Wang
Anna M. Paczulla
Martina Konantz
Claudia Lengerke
8 In Vitro Tumorigenic Assay: Colony Forming Assay for Cancer Stem Cells
89(8)
Vijayalakshmi Rajendran
Mayur Vilas Jain
9 Xenograft as In Vivo Experimental Model
97(10)
Manuela Porru
Luca Pompili
Carla Caruso
Carlo Leonetti
10 How to Assess Drug Resistance in Cancer Stem Cells
107(10)
Maria Laura De Angelis
Ruggero De Maria
Marta Baiocchi
11 Tumor Tissue Analogs for the Assessment of Radioresistance in Cancer Stem Cells
117(12)
Meenakshi Upreti
12 Generation of In Vitro Model of Epithelial Mesenchymal Transition (EMT) Via the Expression of a Cytoplasmic Mutant Form of Promylocytic Leukemia Protein (PML)
129(10)
Anna Di Biase
Amanda K. Miles
Tarik Regad
13 Identification and Isolation of Cancer Stem Cells Using NANOG-EGFP Reporter System
139(10)
Magdalena E. Buczek
Stephen P. Reeder
Tarik Regad
14 Determination of miRNAs from Cancer Stem Cells Using a Low Density Array Platform
149(8)
Hiromichi Kawasaki
Angela Lombardi
Michele Caraglia
15 Assessing DNA Methylation in Cancer Stem Cells
157(22)
Sudipto Das
Bruce Moran
Antoinette S. Perry
16 Histones Acetylation and Cancer Stem Cells (CSCs)
179(16)
Vivian Petersen Wagner
Manoela Domingues Martins
Rogerio Moraes Castilho
17 Immunohistochemistry for Cancer Stem Cells Detection: Principles and Methods
195(18)
Martina Intartaglia
Rosalaura Sabetta
Monica Gargiulo
Giovanna Roncador
Federica Zito Marino
Renato Franco
18 Circulating Tumor Cells
213(8)
Sebastian A. Garcia
Jurgen Weitz
Sebastian Schblch
Index 221
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