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El. knyga: Carbohydrate Analysis: High Performance Liquid Chromatography and Capillary Electrophoresis

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Carbohydrates and glycoconjugates play an important role in several life processes. The wide variety of carbohydrate species and their inherent polydispersity and heterogeneity require separation techniques of high resolving power and high selectivity such as high performance liquid chromatography (HPLC) and capillary electrophoresis (HPCE) In the last decade HPLC, and recently HPCE methods have been developed for the high resolution and reproducible quantitation of carbohydrates. Despite the importance of these two column separation technologies in the area of carbohydrates, no previous book describes specialized methods for the separation, purification and detection of carbohydrates and glycoconjugates by HPLC and HPCE. Therefore, the objective of the present book is to provide a comprehensive review of carbohydrate analysis by HPLC and HPCE by covering analytical and preparative separation techniques for all classes of carbohydrates including mono- and disaccharides; linear and cyclic oligosaccharides; branched heterooligosaccharides (e.g., glycans, plant-derived oligosaccharides); glycoconjugates (e.g., glycolipids, glycoproteins); carbohydrates in food and beverage; compositional carbohydrates of polysaccharides; carbohydrates in biomass degradation; etc. The book will be of interest to a wide audience, including analytical chemists and biochemists, carbohydrate, glycoprotein and glycolipid chemists, molecular biologists, biotechnologists, etc. It will also be a useful reference work for both the experienced analyst and the newcomer as well as for users of HPLC and HPCE, graduates and postdoctoral students.
Part
1. The Solute.
1. Preparation of carbohydrates for analysis by HPLC
and HPCE (A.J. Mort, M.L. Pierce). Part
11. Analytical and Preparative
Separations.
2. Reversed-phase and hydrophobic interaction chromatography of
carbohydrates and glycoconjugates (Z. El Rassi).
3. High performance
hydrophilic interaction chromatography of carbohydrates with polar solvents
(S.C. Churms).
4. HPLC of carbohydrates with cation- and anion-exchange
silica and resin-based stationary phases (C.G. Huber, G.K. Bonn).
5. Analysis
of glycoconjugates using high-pH anionexchange chromatography (R.R.
Townsend).
6. Basic studies on carbohydrate - protein interaction by high
performance affinity chromatography and high performance capillary affinity
electrophoresis using lectins as protein models (S. Honda).
7. Modem size
exclusion chromatography of carbohydrates and glycoconjugates (S.C. Churms).
8. High performance capillary electrophoresis of carbohydrates and
glycoconjugates (Z. El Rassi, W. Nashabeh).
9. Preparative HPLC of
carbohydrates (K.B. Hicks). Part III. The Detection.
10. Pulsed
electrochemical detection of carbohydrates at gold electrodes following
liquid chromatographic separation (D.C. Johnson, W.R. LaCourse). I
1.
On-column refractive index detection of carbohydrates separated by HPLC and
CE (A.E. Bruno, B. Krattiger).
12. Mass spectrometry of carbohydrates and
glycoconjugates (C.A. Settineri, A.L. Burlingame).
13. Evaporative light
scattering detection of carbohydrates in HPLC (M. Dreux, M. Lafosse).
14.
Chiroptical detectors for HPLC of carbohydrates (N. Purdie).
15. Pre- and
post-column detection-oriented derivatization techniques in HPLC of
carbohydrates (S. Hase).
16. Post-column enzyme reactors for the HPLC
determination of carbohydrates -(L.J. Nagels, P.C. Maes).
17. Other direct
and indirect detection methods of carbohydrates in HPLC and HPCE (Z. El
Rassi, J.T. Smith).
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