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Cardiovascular Development: Methods and Protocols 2012 [Kietas viršelis]

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  • Formatas: Hardback, 341 pages, aukštis x plotis: 254x178 mm, weight: 867 g, XIV, 341 p., 1 Hardback
  • Serija: Methods in Molecular Biology 843
  • Išleidimo metai: 06-Jan-2012
  • Leidėjas: Humana Press Inc.
  • ISBN-10: 1617795224
  • ISBN-13: 9781617795220
Kitos knygos pagal šią temą:
  • Formatas: Hardback, 341 pages, aukštis x plotis: 254x178 mm, weight: 867 g, XIV, 341 p., 1 Hardback
  • Serija: Methods in Molecular Biology 843
  • Išleidimo metai: 06-Jan-2012
  • Leidėjas: Humana Press Inc.
  • ISBN-10: 1617795224
  • ISBN-13: 9781617795220
Kitos knygos pagal šią temą:
Including the step-by-step detail essential for reproducible results, this volume deals with the latest techniques in the field of cardiovascular development and includes key notes and implementation advice from experts in this high-profile medical field.

sCongenital heart disease is the leading cause of infant death and affects approximately one in every 100 babies born in the United States. The study of cardiovascular development has acquired new momentum in last twenty years due to the advancement of modern molecular biology and new available equipments and techniques. In Cardiovascular Development: Methods and Protocols expert researchers in the field in the field detail many of the methods which are now commonly used in the field of cardiovascular development research. These include methods and technique for using different organisms for cardiovascular developmental research, using cell and molecular biology methods to study cardiovascular development, as well as other available techniques for cardiovascular development research. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.Authoritative and practical, Cardiovascular Development: Methods and Protocols seeks to aid scientists in understanding new state-of-the-art techniques in the field of cardiovascular development research including in vivo imaging and Bioinformatics.

Recenzijos

From the reviews:

This is an up-to-date review of current research techniques used in the field of cardiovascular development. The book successfully instructs researchers on proper experimental techniques, giving step-by-step instructions, required for research in this field. this book is intended for use by both novice and experienced basic science researchers. This book is an excellent reference for any researcher in this area. (Patrick T. Campbell, Doodys Book Reviews, April, 2012)

Preface v
Contributors xi
PART I Model Organisms
1 Use of Whole Embryo Culture for Studying Heart Development
3(8)
Calvin T. Hang
Ching-Pin Chang
2 Quantifying Cardiac Functions in Embryonic and Adult Zebrafish
11(10)
Tiffany Hoage
Yonghe Ding
Xiaolei Xu
3 Analysis of the Patterning of Cardiac Outflow Tract and Great Arteries with Angiography and Vascular Casting
21(8)
Ching-Pin Chang
4 Morpholino Injection in Xenopus
29(18)
Panna Tandon
Chris Showell
Kathleen Christine
Frank L. Conlon
5 Chicken Chorioallantoic Membrane Angiogenesis Model
47(12)
Domenico Ribatti
6 Visualizing Vascular Networks in Zebrafish: An Introduction to Microangiography
59(10)
Christopher E. Schmitt
Melinda B. Holland
Suk-Won Jin
7 Whole-Mount Confocal Microscopy for Vascular Branching Morphogenesis
69(10)
Yoh-suke Mukouyama
Jennifer James
Joseph Nam
Yutaka Uchida
8 Visualization of Mouse Embryo Angiogenesis by Fluorescence-Based Staining
79(8)
Yang Liu
Marc Antonyak
Xu Peng
9 Miniaturized Assays of Angiogenesis In Vitro
87(14)
May J. Reed
Robert B. Vernon
PART II Cell and Molecular Biology Methods
10 Analysis of the Endocardial-to-Mesenchymal Transformation of Heart Valve Development by Collagen Gel Culture Assay
101(10)
Yiqin Xiong
Bin Zhou
Ching-Pin Chang
11 Quantification of Myocyte Chemotaxis: A Role for FAK in Regulating Directional Motility
111(14)
Britni Zajac
Zeenat S. Hakim
Morgan V. Cameron
Oliver Smithies
Joan M. Taylor
12 Analysis of Neural Crest Cell Fate During Cardiovascular Development Using Cre-Activated lacZ/β-Galactosidase Staining
125(14)
Yanping Zhang
L. Bruno Ruest
13 Indirect Immunostaining on Mouse Embryonic Heart for the Detection of Proliferated Cardiomyocyte
139(8)
Jieli Li
Marc Antonyak
Xu Peng
14 Isolation and Characterization of Vascular Endothelial Cells from Murine Heart and Lung
147(8)
Yixin Jin
Yang Liu
Marc Antonyak
Xu Peng
15 Isolation and Characterization of Embryonic and Adult Epicardium and Epicardium-Derived Cells
155(14)
Bin Zhou
William T. Pu
16 Vascular Smooth Muscle Cells: Isolation, Culture, and Characterization
169(8)
Richard P. Metz
Jan L. Patterson
Emily Wilson
17 C-kit Expression Identifies Cardiac Precursor Cells in Neonatal Mice
177(14)
Michael Craven
Michael I. Kotlikoff
Alyson S. Nadworny
18 Cardiomyocyte Apoptosis in Heart Development: Methods and Protocols
191(8)
Dongfei Qi
Mingui Fu
19 Adenovirus-Mediated Gene Transfection in the Isolated Lymphatic Vessels
199(6)
Anatoliy A. Gashev
Jieli Li
Mariappan Muthuchamy
David C. Zawieja
20 Isolation of Cardiac Myocytes and Fibroblasts from Neonatal Rat Pups
205(12)
Honey B. Golden
Deepika Gollapudi
Fnu Gerilechaogetu
Jieli Li
Ricardo J. Cristales
Xu Peng
David E. Dostal
PART III New Techniques
21 The Application of Genome-Wide RNAi Screens in Exploring Varieties of Signaling Transduction Pathways
217(12)
Shenyuan Zhang
Hongying Zheng
22 Application of Atomic Force Microscopy Measurements on Cardiovascular Cells
229(16)
Xin Wu
Zhe Sun
Gerald A. Meininger
Mariappan Muthuchamy
23 In Utero Assessment of Cardiovascular Function in the Embryonic Mouse Heart Using High-Resolution Ultrasound Biomicroscopy
245(20)
Honey B. Golden
Suraj Sunder
Yang Liu
Xu Peng
David E. Dostal
24 Isolation and Preparation of RNA from Rat Blood and Lymphatic Microvessels for Use in Microarray Analysis
265(26)
Eric A. Bridenbaugh
25 Visual Data Mining of Coexpression Data to Set Research Priorities in Cardiac Development Research
291(18)
Vincent Van Buren
26 High-Speed Confocal Imaging of Zebrafish Heart Development
309(20)
Jay R. Hove
Michael P. Craig
27 Measurement of Electrical Conduction Properties of Intact Embryonic Murine Hearts by Extracellular Microelectrode Arrays
329(10)
David G. Taylor
Anupama Natarajan
Index 339