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El. knyga: Cardiovascular Development: Methods and Protocols

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  • Formatas: EPUB+DRM
  • Serija: Methods in Molecular Biology 2319
  • Išleidimo metai: 30-Jul-2021
  • Leidėjas: Springer-Verlag New York Inc.
  • Kalba: eng
  • ISBN-13: 9781071614808
Kitos knygos pagal šią temą:
  • Formatas: EPUB+DRM
  • Serija: Methods in Molecular Biology 2319
  • Išleidimo metai: 30-Jul-2021
  • Leidėjas: Springer-Verlag New York Inc.
  • Kalba: eng
  • ISBN-13: 9781071614808
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This second edition brings together a variety of method used in the field of cardiovascular development research. Chapters guide readers through protocols on lymphatic development, lymphatic staining techniques, lymphatic endothelial, tissue clearing techniques, and muscle cell isolation and culture. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls

Authoritative and practical, Cardiovascular Development: Methods and Protocols, Second Edition seeks to aid scientists in understanding new state-of-the-art techniques in the field of cardiovascular development research.

Tissue clearing and 3-D visualization of Vasculature with the PEGASOS
Method.- Preparation and Identification of Cardiac Myofibrils from Whole
Heart Sample.- RNA-Sequencing and Transcriptome Analysis after Fluid Shear
Stress Stimulation in Lymphatic Endothelial Cells.- Preparation of Neonatal
Rat Papillary Muscles for Contractile Studies.- In Vitro Study of
permeability in Lymphatic Endothelial Cells Responding to
Histamine.- Optimizing the Differentiation of Cardiomyocytes from Human
Induced Pluripotent Derived-Stem Cells.- Analysis of Lymphatic Vessel
Formation by Whole-mount Immunofluorescence Staining.- Isolation and Culture
of Mouse Lymphatic Endothelial Cells from Lung Tissue.- Laser-induced
Choroidal Neovascularization in Rats.- Visualizing Three-Dimensional Vascular
Networks in the Mouse Embryonic Hindbrain.- Visualizing Blood Vessel
Development in Cultured Mouse Embryos Using Lightsheet Microscopy.- Laser
Capture Microdissection of Vascular Endothelial Cells from Frozen Heart
Tissues.- Visualization of Fundus Blood Vessels.- Transient Transgenics: An
Efficient Method to Identify Gene Regulatory Elements.- Isolation of
Lymphatic Muscle Cells (LMCs) from Rat Mesentery.- Isolation of Adult Mouse
Cardiomyocytes using Langendorff Perfusion Apparatus.- Visualization of
Angiogenesis in Mouse Embryonic Dorsal Skin by Whole-Mount Fluorescent
Staining.