Atnaujinkite slapukų nuostatas

Cholesterol Homeostasis: Methods and Protocols 1st ed. 2017 [Kietas viršelis]

4.00/5 (2 ratings by Goodreads)
Edited by , Edited by
  • Formatas: Hardback, 300 pages, aukštis x plotis: 254x178 mm, weight: 7392 g, 37 Illustrations, color; 27 Illustrations, black and white; XI, 300 p. 64 illus., 37 illus. in color. With online files/update., 1 Hardback
  • Serija: Methods in Molecular Biology 1583
  • Išleidimo metai: 17-Feb-2017
  • Leidėjas: Humana Press Inc.
  • ISBN-10: 1493968734
  • ISBN-13: 9781493968732
Kitos knygos pagal šią temą:
Cholesterol Homeostasis: Methods and Protocols 1st ed. 2017Didesnis paveikslėlis
  • Formatas: Hardback, 300 pages, aukštis x plotis: 254x178 mm, weight: 7392 g, 37 Illustrations, color; 27 Illustrations, black and white; XI, 300 p. 64 illus., 37 illus. in color. With online files/update., 1 Hardback
  • Serija: Methods in Molecular Biology 1583
  • Išleidimo metai: 17-Feb-2017
  • Leidėjas: Humana Press Inc.
  • ISBN-10: 1493968734
  • ISBN-13: 9781493968732
Kitos knygos pagal šią temą:
Pastovi nuoroda: https://www.kriso.lt/db/9781493968732.html
Raktažodžiai:

This volume provides state-of-the-art techniques for studying various aspects of cholesterol homeostasis, including its uptake, synthesis and efflux from the cell, as well as its trafficking within the cell.  Chapters also cover techniques for studying the regulation of cholesterol homeostasis at both the transcriptional and post-translational levels, as well as studying the membrane topology and structure of cholesterol-related proteins. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Cholesterol Homeostasis: Methods and Protocols aims to provide key techniques in tackling the investigation of cholesterol homeostasis.

Recenzijos

The multicountry authors of this volume review present knowledge on the multiple basic roles of cholesterol in the living body. Each chapter details the methods of studies performed, backed by many references. For biologists, physiologists, biochemists. (Zvi Laron, Pediatric Endocrinology Reviews (PER), Vol. 15 (1), September, 2017)

Preface v
Contributors ix
1 An Overview of Cholesterol Homeostasis
1(6)
Ingrid C. Gelissen
Andrew J. Brown
2 Hybrid In Silico/In Vitro Approaches for the Identification of Functional Cholesterol-Binding Domains in Membrane Proteins
7(14)
Coralie Di Scala
Jacques Fantini
3 Structural Stringency of Cholesterol for Membrane Protein Function Utilizing Stereoisomers as Novel Tools: A Review
21(20)
Md. Jafurulla
Amitabba Chattopadhyay
4 Manipulating Cholesterol Status Within Cells
41(12)
Winnie Luu
Ingrid C. Gelissen
Andrew J. Brown
5 Assaying Low-Density-Lipoprotein (LDL) Uptake into Cells
53(12)
Anke Loregger
Jessica K. Nelson
Noam Zelcer
6 The Use of L-sIDOL Transgenic Mice as a Murine Model to Study Hypercholesterolemia and Atherosclerosis
65(8)
Eser J. Zerenturk
Anna C. Calkin
7 CRISPR/Cas9-mediated Generation of Niemann-Pick C1 Knockout Cell Line
73(12)
Ximing Du
Ivan Lukmantara
Hongyuan Tang
8 Quantitative Measurement of Cholesterol in Cell Populations Using Flow Cytometry and Fluorescent Perfringolysin O*
85(12)
Jian Li
Peter L. Lee
Suzanne R. Pfeffer
9 Transport Assays for Sterol-Binding Proteins: Stopped-Flow Fluorescence Methods for Investigating Intracellular Cholesterol Transport Mechanisms of NPC2 Protein
97(14)
Leslie A. McCauliff
Judith Storch
10 Synthesis and Live-cell Imaging of Fluorescent Sterols for Analysis of Intracellular Cholesterol Transport
111(30)
Maciej Modzel
Frederik W. Lund
Daniel Wustner
11 Measurement of Cholesterol Transfer from Lysosome to Peroxisome Using an In Vitro Reconstitution Assay
141(22)
Jie Luo
Ya-Cheng Liao
Jian Xiao
Bao-Liang Song
12 Measurement of Mitochondrial Cholesterol Import Using a Mitochondria-Targeted CYP11A1 Fusion Construct
163(22)
Barry E. Kennedy
Mark Charman
Barbara Karten
13 Identifying Sterol Response Elements Within Promoters of Genes
185(8)
Laura J. Sharpe
Andrew J. Brown
14 Membrane Extraction of HMG CoA Reductase as Determined by Susceptibility of Lumenal Epitope to In Vitro Protease Digestion
193(8)
Lindsey L. Morris
Russell A. DeBose-Boyd
15 Determining the Topology of Membrane-Bound Proteins Using PEGylation
201(10)
Vicky Howe
Andrew J. Brown
16 Measuring Activity of Cholesterol Synthesis Enzymes Using Gas Chromatography/Mass Spectrometry
211(10)
Anika V. Prabhu
Winnie Luu
Andrew J. Brown
17 Sterol Analysis by Quantitative Mass Spectrometry
221(20)
Andrew M. Jenner
Simon H.J. Brown
18 Measurement of Rates of Cholesterol and Fatty Acid Synthesis In Vivo Using Tritiated Water
241(16)
Adam M. Lopez
Jen-Chieh Chuang
Stephen D. Turley
19 Methods for Monitoring ABCA1-Dependent Sterol Release
257(18)
Yoshio Yamauchi
Shinji Yokoyama
Ta-Yuan Chang
20 ABC-Transporter Mediated Sterol Export from Cells Using Radiolabeled Sterols
275(12)
Alryel Yang
Ingrid C. Gelissen
21 Measurement of Macrophage-Specific In Vivo Reverse Cholesterol Transport in Mice
287(12)
Wendy Jessup
Maaike Kockx
Leonard Kritharides
Index 299