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El. knyga: Chromothripsis: Methods and Protocols

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  • Formatas: EPUB+DRM
  • Serija: Methods in Molecular Biology 1769
  • Išleidimo metai: 21-Mar-2018
  • Leidėjas: Humana Press Inc.
  • Kalba: eng
  • ISBN-13: 9781493977802
Kitos knygos pagal šią temą:
Chromothripsis: Methods and ProtocolsDidesnis paveikslėlis
  • Formatas: EPUB+DRM
  • Serija: Methods in Molecular Biology 1769
  • Išleidimo metai: 21-Mar-2018
  • Leidėjas: Humana Press Inc.
  • Kalba: eng
  • ISBN-13: 9781493977802
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This detailed book presents an up-to-date view on methods and experimental approaches developed to identify and explore the chromothripsis phenomenon. Beginning with a section exploring the genesis and impact of chromothripsis, the collection continues by covering the identification of chromothripsis, the causal mechanisms of chromothripsis, the bioinformatics tools for chromothripsis analysis, and experimental systems recently developed for the in vitro investigation of chromothripsis. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Chromothripsis: Methods and Protocols serves as a vital resource for cell biologists, molecular biologists, cytogeneticists, and geneticists investigating chromothripsis, but also for students and researchers new to the field of chromothripsis and genomic instability.
The Genomic Characteristics and Origin of Chromothripsis.- Clinical
Consequences of Chromothripsis and Other Catastrophic Cellular Events.-
Potential Role of Chromothripsis in the Genesis of Complex Chromosomal
Rearrangements in Human Gametes and Preimplantation Embryo.- Chromothripsis
and the Macroevolution Theory.- Analysis of Chromothripsis by Combined FISH
and Microarray Analysis.- Chromothripsis Detectable in Small Supernumerary
Marker Chromosomes (sSMC) Using Fluorescence In Situ Hybridization (FISH).-
Identification of Chromothripsis in Biopsy Using SNP-Based Microarray.-
Detection of Chromothripsis in Plants.- RNA-Seq Analysis to Detect Abnormal
Fusion Transcripts Linked to Chromothripsis.- Experimental Determination of
Checkpoint Adaptation by Mitotic Shake-Off and Microscopy.- A Role for
Retrotransposons in Chromothripsis.- Generation of Micronuclei and Detection
of Chromosome Pulverization.- Detection of Impaired DNA Replication and
Repair in Micronuclei as Indicators of Genomic Instability and
Chromothripsis.- Study of Telomere Dysfunction in TP53 Mutant LoVo Cell Lines
as a Model for Genomic Instability.- Genes, Proteins, and Biological Pathways
Preventing Chromothripsis.- Expression of Genes Associated with Telomere
Homeostasis in TP53 Mutant LoVo Cell Lines as a Model for Genomic
Instability.- Chromothripsis Detection and Characterization Using the
CTLPScanner Web Server.- ChromothripsisDB: A Curated Database for the
Documentation, Visualization, and Mining of Chromothripsis Data.- Time-Lapse
Imaging for the Detection of Chromosomal Abnormalities in Primate
Pre-Implantation Embryos.- Correlative Live Imaging and Immunofluorescence
for Analysis of Chromosome Segregation in Mouse Preimplantation Embryos.-
Experimental Induction of Genome Chaos.- Looking for Broken TAD Boundaries
and Changes on DNA Interactions: Clinical Guide to 3D Chromatin Changes
Analysis in Complex Chromosomal Rearrangements and Chromothripsis.
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