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Circadian Clocks: Methods and Protocols 2021 ed. [Minkštas viršelis]

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  • Formatas: Paperback / softback, 342 pages, aukštis x plotis: 254x178 mm, weight: 692 g, 53 Illustrations, color; 11 Illustrations, black and white; XXII, 342 p. 64 illus., 53 illus. in color., 1 Paperback / softback
  • Serija: Methods in Molecular Biology 2130
  • Išleidimo metai: 09-Dec-2021
  • Leidėjas: Springer-Verlag New York Inc.
  • ISBN-10: 1071603833
  • ISBN-13: 9781071603833
Kitos knygos pagal šią temą:
  • Formatas: Paperback / softback, 342 pages, aukštis x plotis: 254x178 mm, weight: 692 g, 53 Illustrations, color; 11 Illustrations, black and white; XXII, 342 p. 64 illus., 53 illus. in color., 1 Paperback / softback
  • Serija: Methods in Molecular Biology 2130
  • Išleidimo metai: 09-Dec-2021
  • Leidėjas: Springer-Verlag New York Inc.
  • ISBN-10: 1071603833
  • ISBN-13: 9781071603833
Kitos knygos pagal šią temą:

This volume presents techniques used by researchers from all branches of biology to study daily changes at a molecular level in many physiological systems. The chapters are organized into three parts and cover topics such as measuring and modeling physiological and behavioral rhythms; genome-wide analyses in circadian biology, and imaging and manipulating brain clocks. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and practical, Circadian Clocks: Methods and Protocols is a valuable tool for any researcher interested in learning more about this developing field.


Real-Time In Vitro Fluorescence Anisotropy of the Cyanobacterial
Circadian Clock.- Inductively Coupled Plasma Mass Spectrometry for Elemental
Analysis in Circadian Biology.- The Assessment of Circadian Rhythms within
the Immune System.- Measuring Circadian Rhythms in Human Cells.- Measuring
the Effects of Circadian Rhythm-Related Manipulations on Depression-Like
Behavior in Rodents: Forced Swim and Tail Suspension Tests.- Asking the
Clock: How to Use Information from Questionnaires for Circadian
Phenotyping.- Simple Kinetic Models in Molecular Chronobiology.- Searching
Novel Clock Genes using RNAi-Based Screening.- Chromatin Immunoprecipitation
(ChIP) from Mouse Liver Nuclei.- Using GRO-seq to Measure Circadian
Transcription and Discover Circadian Enhancers.- Circadian Metabolomics from
Breath.- A Lipidomics View of Circadian Biology.- Circadian Lipidomics:
Analysis of Lipid Metabolites Around-The-Clock.- Phosphoproteome and Proteome
Sample Preparation from Mouse Tissues for Circadian Analysis.- Circadian
Phosphorylation of CLOCK and BMAL1.- Fluorescence Live Imaging of Drosophila
Circadian Pacemaker Neurons.- Monitoring Electrical Activity in Drosophila
Circadian Output Neurons.- Light Input to the Mammalian Circadian
Clock.- Acute In Vivo Multi-Electrode Recordings from the Mouse
Suprachiasmatic Nucleus.- Perforated Multi-Electrode Array Recording in
Hypothalamic Brain Slices.- Collection of Mouse Brain Slices for
Bioluminescence Imaging of Circadian Clock Networks.- Computational Analysis
of PER2::LUC Imaging Data.- Electrophysiological Approaches to Studying the
Suprachiasmatic Nucleus.- Optogenetic Methods for the Study of Circadian
Rhythms.