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El. knyga: Clostridium difficile: Methods and Protocols

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  • Formatas: PDF+DRM
  • Serija: Methods in Molecular Biology 646
  • Išleidimo metai: 03-Jul-2010
  • Leidėjas: Humana Press Inc.
  • Kalba: eng
  • ISBN-13: 9781603273657
  • Formatas: PDF+DRM
  • Serija: Methods in Molecular Biology 646
  • Išleidimo metai: 03-Jul-2010
  • Leidėjas: Humana Press Inc.
  • Kalba: eng
  • ISBN-13: 9781603273657

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Clostridium difficile, a major nosocomial pathogen shown to be a primary cause of antibiotic-associated disease, has emerged as a highly transmissible and frequently antibiotic-resistant organism, causing a considerable burden on health care systems worldwide. In Clostridium difficile: Methods and Protocols, expert researchers bring together the most recently developed methods for studying the organism, including techniques involving isolation, molecular typing, genomics, genetic manipulation, and the use of animal models.  Written in the highly successful Methods in Molecular Biology series format, chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes highlighting tips on troubleshooting and avoiding known pitfalls.



 



Authoritative and cutting-edge, Clostridium difficile: Methods and Protocols serves as an ideal guide for scientists now in a position to gain an in-depth understanding of how this organism is transmitted and how it causes disease.

Recenzijos

From the reviews:

This book describes a variety of research methods used to investigate the pathogenic properties of Clostridium difficile, a major nosocomial pathogen that causes a severe diarrhea after prolonged antibiotic treatment. This book would be useful to scientists working with bacteria such as C. difficile that cause toxin-mediated diseases in the colon of their host. This is a technical book that provides essential methods and techniques to study devastating diseases due to Clostridium difficile. (Rebecca T. Horvat, Doodys Review Service, October, 2010)

Preface v
Contributors ix
PART I INTRODUCTION TO CLOSTRIDIUM DIFFICILE AND THE DISEASE IT CAUSES
1 Clostridium difficile: No Longer and Enigmatic Pathogen?
3(6)
Adam P. Roberts
Peter Mullany
2 Clostridium difficile and the Disease it Causes
9(30)
Torbjorn Noren
PART II ISOLATION AND CULTURE TECHNIQUES
3 Clostridium difficile Isolation and Culture Techniques
39(16)
Mike Wren
PART III METHODS FOR TYPING AND EPIDEMIOLOGICAL STUDIES
4 Molecular Typing Methods for Clostridium difficile: Pulsed-Field Gel Electrophoresis and PCR Ribotyping
55(12)
Sandra Janezic
Maja Rupnik
5 Clostridium difficile Toxinotyping
67(10)
Maja Rupnik
6 Multilcous Sequence Typing for Clostridium difficile
77(16)
Ludovic Lemee
Jean-Louis Pons
PART IV BIOCHEMISTRY OF THE ORGANISM
7 Molecular Methods to Study Transcriptional Regulation of Clostridium difficile Toxin Genes
93(24)
Ana Antunes
Bruno Dupuy
8 Dissecting the Cell Surface
117(18)
Robert Fagan
Neil Fairweather
9 Human Intestinal Epithelial Response(s) to Clostridium difficile
135(14)
Nazila V. Jafari
Elaine Allan
Mona Bajaj-Elliott
PART V GENOMICS
10 Comparative Genome Analysis of Clostridium difficile Using DNA Microarrays
149(16)
Richard Stabler
Lisa Dawson
Brendan Wren
PART VI DEVELOPMENT OF SYSTEMS FOR GENETIC ANALYSIS OF THE ORGANISM
11 ClosTron-Targeted Mutagenesis
165(18)
John T. Heap
Stephen T. Cartman
Sarah A. Kuebne
Clare Cooksley
Nigel P. Minton
12 Methods for Gene Cloning and Targeted Mutagenesis
183(20)
Glen P. Carter
Dena Lyras
Rachael Poon
Pauline M. Howarth
Julian I. Rood
13 Transposon Mutagenesis in Clostridium difficile
203(12)
Haitham A. Hussian
Adam P. Roberts
Rachael Whalan
Peter Mullany
PART VII ANIMAL MODELS OF DISEASE
14 Refinement of the Hamster Model of Clostridium difficile Disease
215(14)
Gillian Douce
David Goulding
15 Methods for Working with the Mouse Model
229(10)
Anne Collignon
Subject Index 239