"The field of biotechnology is advancing at a faster pace. The availability of low-cost DNA/genome sequencing technologies has led to the discovery and functional characterization of myriad of genes imparting stress tolerance and quality traits. 'OMICS' group of technologies including Genomics, Proteomics, Transcriptomics and Metabolomics has revolutionized the agricultural biotechnology sector. The Nobel Prize winning technology i.e. Genome Editing technique is being employed to edit various gene functions in plants aiding in crop improvement. This technology may be adopted very fastly by the consumers as compared to the transgenic technique as the genome-edited plants do not have any adverse effects on the genome of the plants itself and also on the environment and related species/non-target organisms. In this book, authors have attempted to compile various latest techniques of agricultural biotechnology and their applications in crop improvement. Certain specific chapters have been dedicated to describe the use of Nanotechnology, a fast emerging new technique in agriculture sector. The book offers a comprehensive coverage of the most essential topics including; Development, potential and safety issues in biotechnology Advances in Genomics, Proteomics and Transcriptomics in agriculture Protein bioinformatics and its applications GM technology and its implications Genome Editing in crop improvement MAS in crop improvement Mutation breeding Cryobiotechnology Nanotechnology and Biosensors This book includes real-world examples and applications making the book accessible to a broader interdisciplinary readership. We hope that it will serve as a reference book for Researchers engaged in Molecular Biology & Biotechnology and will act as a ready reckoner for PG students of Biotechnology discipline"--
The field of biotechnology is advancing at a faster pace. The availability of low-cost DNA/genome sequencing technologies has led to the discovery and functional characterization of myriad of genes imparting stress tolerance and quality traits. OMICS group of technologies including Genomics, Proteomics, Transcriptomics and Metabolomics has revolutionized the agricultural biotechnology sector. The Nobel Prize winning technology i.e. Genome Editing technique is being employed to edit various gene functions in plants aiding in crop improvement. This technology may be adopted very fastly by the consumers as compared to the transgenic technique as the genome-edited plants do not have any adverse effects on the genome of the plants itself and also on the environment and related species/non-target organisms.
In this book, authors have attempted to compile various latest techniques of agricultural biotechnology and their applications in crop improvement. Certain specific chapters have been dedicated to describe the use of Nanotechnology, a fast emerging new technique in agriculture sector.
The book offers a comprehensive coverage of the most essential topics including;
- Development, potential and safety issues in biotechnology
- Advances in Genomics, Proteomics and Transcriptomics in agriculture
- Protein bioinformatics and its applications
- GM technology and its implications
- Genome Editing in crop improvement
- MAS in crop improvement
- Mutation breeding
- Cryobiotechnology
- Nanotechnology and Biosensors
This book includes real-world examples and applications making the book accessible to a broader interdisciplinary readership. We hope that it will serve as a reference book for Researchers engaged in Molecular Biology & Biotechnology and will act as a ready reckoner for PG students of Biotechnology discipline.
| Preface |
|
ix | |
| Editors |
|
xi | |
| Contributors |
|
xiii | |
|
1 Biotechnology in Agriculture: Development, Potentials and Safety |
|
|
1 | (22) |
|
|
|
2 Advances in Genomics and Proteomics in Agriculture |
|
|
23 | (14) |
|
|
|
|
|
|
|
|
|
|
|
|
|
3 Integrated Metabolome and Transcriptome Analysis: A New Platform/Technology for Functional Biology and Natural Products Research |
|
|
37 | (16) |
|
|
|
|
|
|
|
|
|
4 Recent Advances in Protein Bioinformatics |
|
|
53 | (10) |
|
|
|
|
|
|
|
5 CBL-CIPK: The Ca+ Signals during Abiotic Stress Response |
|
|
63 | (12) |
|
|
|
|
|
6 Transgenic Technology and Its Progressive Implications |
|
|
75 | (6) |
|
|
|
|
|
|
|
7 PR Proteins: Key Genes for Engineering Disease Resistance in Plants |
|
|
81 | (18) |
|
|
|
|
|
|
|
|
|
|
|
8 Approaches and Techniques in Plant Metabolic Engineering |
|
|
99 | (12) |
|
|
|
9 Genome Editing for Crop Improvement |
|
|
111 | (14) |
|
|
|
|
|
|
|
|
|
|
|
|
|
10 Molecular Marker-Assisted Breeding for Crop Improvement |
|
|
125 | (14) |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
11 Biotechnological Advances and Advanced Mutation Breeding Techniques for Crop Plants |
|
|
139 | (28) |
|
|
|
|
|
|
|
12 Recent Advancement of Nanotechnology in Agriculture |
|
|
167 | (12) |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
13 Cryobiotechnology in Plants: Recent Advances and Prospects |
|
|
179 | (18) |
|
|
|
|
|
14 Role of Biotechnology in the Improvement of Cole Crops |
|
|
197 | (14) |
|
|
|
|
|
|
|
15 Application of Nanotechnology in Crop Improvement: An Overview |
|
|
211 | (14) |
|
|
|
|
|
16 Biosensors in Food Industry: An Exposition of Novel Food Safety Approach |
|
|
225 | (12) |
|
|
|
|
|
|
|
|
|
|
|
17 Advances in Microbes Use in Agricultural Biotechnology |
|
|
237 | (8) |
|
|
|
|
|
|
|
|
| Index |
|
245 | |
Dr. Pankaj Kumar is presently working as Assistant Professor (Biotechnology), Department of Biotechnology, Dr. Y.S. Parmar University of Horticulture and Forestry, Solan, Himachal Pradesh, India. Formerly, he has worked as SERB- National Post Doctoral Fellow (DST Young Scientist Scheme) at Council of Scientific & Industrial Research - Institute of Himalayan Bioresource Technology, Palampur, Himachal Pradesh. India. Dr. Kumar is working on plant secondary metabolite enhancement through cell and tissue engineering approaches; Identification of molecular cues linked with enhanced metabolites using comparative transcriptomics approach and also working on hydro-aero cultivation of medicinal plants for industrial importance. During Doctoral Studies; he (research team) has generated technology at Dr Yashwant Singh Parmar University of Horticulture & Forestry Nauni, Solan for developing insect pest resistant transgenic plants in economically important vegetable crops of cauliflower cv. Pusa Snowball, cabbage cv. Pride of India and broccoli cv. Solan Green Head (with cryIAa gene) for insect pest resistance. He has been awarded DST INSPIRE JRF/SRF Fellowship, Department of Science and Technology, Ministry of Science and Technology, Government of India for Ph.D. full doctoral program. He has qualified ICAR AICE-SRF (PGS), ICAR ASRB National Eligibility Test (NET). He has published 32 research/review papers in various journals of International and National repute, contributed 13 book chapters and 5 popular articles. Dr. Kumar has received many awards from various societies and scientific organizations for his scientific contribution i.e. Young Scientist Award Biotechnology-2018 by Society for Plant Research, Young Scientist Award -2019 by the Society of Tropical Agriculture, New Delhi, India, Excellence in Research Award -2019 by Agro Environmental Development Society, India. etc.
Dr. Ajay Kumar Thakur is presently working as Senior Scientist (Biotechnlogy) at ICAR-Directorate of Rapeseed-Mustard Research, Bharatpur, Rajasthan. He has obtained his graduation, PG and Doctoral degrees from Dr. Y.S. Parmar University of Horticulture & Forestry, Solan, H.P. He got induced into Agricultural Research Services (ARS) in 2008. He has published 40 research/review papers in various journals of International and National repute, authored one book Agricultural Biotechnology at a Glance, contributed 8 book chapters and 22 popular articles. He has developed high efficiency plant regeneration and genetic transformation protocols in a number of crops including Populus ciliata, P. deltoides, Punica granatum, Capsicum annum and Cucumis sativus. Dr. Thakur is associated with Brassica juncea improvement programme using biotechnological interventions from last eleven years. He has developed a core set of SSR markers for B. juncea genomics and is presently working on germplasm characterization and association mapping of various agronomically important traits in this oilseed crop. He has also filed one patent and associated in the development of a high yielding Indian mustard variety Giriraj, a white rust resistant Indian mustard genetic stock DRMR MJA 35, which is a Moricandia system- based cytoplasmic male sterile line of B. juncea, and a multiple disease (Alternaria blight, white rust and powdery mildew) resistant Indian mustard genetic stock, DRMRIJ 12-48. Dr. Thakur has received many awards from various societies and scientific organizations for his scientific contribution. He is also an elected Member of Plant Tissue Culture Association of India.
