Baron (hematology-oncology, Mount Sinai School of Medicine) surveys methods available to study hematopoiesis in fruit flies, frogs, fish, mice, chicks, and humans. Techniques described range from the genetic, molecular, and cellular, to cell and embryo explant culture. Bioinformatic and functional genomic approaches for studying stem cells and their supportive stromal cells are also discussed. Protocols include step-by-step instructions, underlying principles, lists of equipment and reagents, and tips on avoiding common problems. The audience for the book includes novice and experienced cell, molecular, and developmental biologists, geneticists, and hematologists. Annotation ©2004 Book News, Inc., Portland, OR (booknews.com)
Part I. Genetic Approaches; Gene Targeting and Transgenic Strategies for
Analysis of Hematopoietic Development in the Mouse; Hanna K.A. Mikkola and
Stuart H. Orkin; Inducible Transgene Expression in Mouse ES Cells; David T.
Ting, Michael Kyba, and George Q. Daley; Quantitative Trait Analysis in the
Investigation of Function and Aging in Hematopoietic Stem Cells; Hans-Willem
Snoeck; Part II. Analysis of Cell Fate Outcomes in Transplantation Models;
Flow Cytometric Analysis of Hematopoietic Development; Edward F. Srour and
Mervin C. Yoder; Reconstitution of Hematopoiesis Following Intrauterine
Transplantation of Stem Cells; Elisabeth H. Javazon, Aziz M. Merchant, Enrico
Danzer, and Alan W. Flake; Reconstitution of Hematopoiesis Following
Transplantation into Neonatal Mice; Scott A. Johnson and Mervin C. Yoder;
Part III. Animal Models; Drosophila; Hemocyte Development during Drosophila
Embryogenesis; Robert A. Schulz and Nancy Fossett; Xenopus; Tracking and
Programming Early Hematopoietic Cells in Xenopus Embryos; Maggie Walmsley,
Aldo Ciau-Uitz, and Roger Patient; Fate Mapping Hematopoietic Lineages in the
Xenopus Embryo; Mary Constance Lane and Michael D. Sheets; Analyses of Immune
Responses to Ontogeny-Specific Antigens Using an Inbred Strain of Xenopus
laevis (J strain); Yumi Izutsu and Mitsugu Maeno; Use of flow cytometry and
combined DNA/Surface staining for analysis of hematopoietic development in
the Xenopus embryo; James B. Turpen; Zebrafish; Analysis of Hematopoietic
Development in the Zebrafish Embryo; Noelle N. Paffett-Lugassy and Leonard I.
Zon; Imaging Early Macrophage Differentiation, Migration and Behaviours in
Live Zebrafish Embryos; Philippe Herbomel and Jean-Pierre Levraud; Chick; In
Vivo Methods to Analyze Cell Origins, Migrations, Homing, and Interactions in
the Blood, Vascular, and Immune; Systems of the Avian and Mammalian Embryo;
Francoise Dieterlen-Lievre, Sophie Creuzet, and Josselyne Salaun; Mouse;
Mouse Embryonic Explant Culture System for Analysis of Hematopoietic and
Vascular Development; Margaret H. Baron and Deanna Mohn; Hematopoietic Stem
Cell Enrichment from the AGM Region of the Mouse Embryo; Catherine Robin and
Elaine Dzierzak; Hematopoietic Stem Cell Development during Mouse
Embryogenesis; Julien Y. Bertrand, Sebastien Giroux, Ana Cumano, and Isabelle
Godin; Analysis of Hematopoietic Progenitors in the Mouse Embryo; James Palis
and Anne Koniski; In Vivo and In Vitro Assays of Thymic Organogenesis; Julie
Gordon, Valerie A. Wilson, Billie A. Moore-Scott, Nancy R. Manley, and C.
Clare Blackburn; Retroviral Transduction in Fetal Thymic Organ Culture;
Bronwyn M. Owens, Robert G. Hawley, and Lisa M. Spain; Expansion and
Differentiation of Immature Mouse and Human Hematopoietic Progenitors; Helmut
Dolznig, Andrea Kolbus, Uwe Schmidt, Cornelia Strobl, Eva-Maria Deiner, Ernst
W. Mullner, and Hartmut Beug; A New Clonal Assay System for Lymphoid and
Myeloid Lineages; Hiroshi Kawamoto and Yoshimoto Katsura; Hematopoietic and
Endothelial Development of Mouse Embryonic Stem Cells in Culture; Kyunghee
Choi, Yun Shin Chung, and Weh Jie Zhang; Analysis of the Endothelial
Potential of Hematopoietic Stem Cells; Steven M. Guthrie, Sergio Caballero,
Robert N. Mames, Maria B. Grant, and Edward W. Scott; Dynamic In Vivo Imaging
of Mammalian Hemato-Vascular Development Using Whole Embryo Culture;
Elizabeth A. V. Jones, Margaret H. Baron, Scott E. Fraser, and Mary E.
Dickinson; Fluorescent protein-cell labeling and its application in
time-lapse analysis of hematopoietic differentiation; Matthias Stadtfeld,
Florencio Varas, and Thomas Graf; Human
