DNA Sequencing III: Dealing With Difficult Templates: Dealing with Difficult Templates [Kietas viršelis]

Sanger sequencing chemistry is already considered a mature technology, having produced complete sequencing of hundreds of microbial species and many higher organisms. Other technologies may be appearing on the horizon, but many researchers have neither the need nor the resources to make use of them at present. This collection is likely to make new technologies even less attractive in comparison to the Sanger method as contributors explain techniques researchers can use in the five to eight percent of cases in which the template proves to be difficult. The eight papers here cover controlled heat denaturation of DNA plasmids and PCR fragments, heat denaturation in sequence of difficult DNA templates, solutions for sequencing difficult regions, improving sequencing results with a commercially available sequence resolver kit, sequencing through secondary structures, transcriptional sequencing as a tool for reading, bas-free cloning of "unclonable" DNA for simplified genomic finishing, and bioinformatics tools to aid sequencing. Annotation ©2008 Book News, Inc., Portland, OR (booknews.com)