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Duchenne Muscular Dystrophy: Methods and Protocols [Kietas viršelis]

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  • Formatas: Hardback, 488 pages, aukštis x plotis: 254x178 mm, 41 Illustrations, color; 16 Illustrations, black and white; Approx. 490 p. 57 illus., 41 illus. in color., 1 Hardback
  • Serija: Methods in Molecular Biology 2975
  • Išleidimo metai: 19-Oct-2025
  • Leidėjas: Springer-Verlag New York Inc.
  • ISBN-10: 1071648101
  • ISBN-13: 9781071648100
Kitos knygos pagal šią temą:
Duchenne Muscular Dystrophy: Methods and ProtocolsDidesnis paveikslėlis
  • Formatas: Hardback, 488 pages, aukštis x plotis: 254x178 mm, 41 Illustrations, color; 16 Illustrations, black and white; Approx. 490 p. 57 illus., 41 illus. in color., 1 Hardback
  • Serija: Methods in Molecular Biology 2975
  • Išleidimo metai: 19-Oct-2025
  • Leidėjas: Springer-Verlag New York Inc.
  • ISBN-10: 1071648101
  • ISBN-13: 9781071648100
Kitos knygos pagal šią temą:
Pastovi nuoroda: https://www.kriso.lt/db/9781071648100.html
This volume discusses protocols for key laboratory techniques used to study Duchenne and Becker muscular dystrophies. The chapters in this book cover a wide array of topics such as biochemical assays, quantification of dystrophin and related proteins, and measurements of muscle physiology. The chapters also cover methods of DMD sequence analysis; processing and isolation of cells from muscle tissue; culture and study of patient-derived cells; and work with animal models of DMD. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Comprehensive and authoritative, Duchenne Muscular Dystrophy: Methods and Protocols is a valuable resource that will contribute to the standardization of practices in the study of dystrophin and dystrophin-related disease.



 
Multiplex Ligation-Dependent Probe Amplification (MLPA) for the
Detection of Copy Number Mutations in the DMD Gene.- Total RNA-seq as a Tool
to Study DMD Splicing and Transcriptional Dynamics.- Preparation, Sectioning,
and Histochemical Staining of Muscle Tissue.- Manual Immunofluorescence
Staining for Dystrophin and Key Dystrophin-Associated Proteins.- Automated
Quantification of Dystrophin Expression by Immunofluorescence in Humans and
Animal Models.- Quantitative Evaluation of Dystrophin Expression Using
SDS-PAGE Western Blot Methods.- Analysis of Dystrophin-Associated
Glycoproteins: Focus on Expression and Glycosylation of Dystroglycan in
Skeletal Muscle.- Quantification of Dystrophin Expression by Capillary
Immunoassay.- Quantification of Dystrophin in Human Muscle Biopsies by Mass
Spectrometry.- Isolation and Culture of Primary Myoblasts from Humans and
Mice.- Isolation of Immune Cells from Skeletal Muscles for Flow Cytometry.-
Exploring Therapies for Duchenne Muscular Dystrophy using Transdifferentiated
Patient Fibroblasts.- Culture and Electrophysiological Analysis of
Patient-Specific iPSCs using Microelectrode Array Dishes.- Canine Model of
Duchenne Muscular Dystrophy.- In Vivo Electroporation of Plasmid DNA into the
Skeletal Muscle of Dystrophic Mouse Models.- Intramuscular and Intravenous
AAV-Mediated Gene Delivery in Mouse Models.- Assessment of Contractile and
Kinetic Properties of Skeletal and Cardiac Multicellular Preparations in
Mouse Models: A Comprehensive Methodological Guide.