| Contributors |
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xiii | |
| Preface |
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xix | |
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1 Current status and future development of plastics: Solutions for a circular economy and limitations of environmental degradation |
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1 | (26) |
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2 | (1) |
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3 | (1) |
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3 Biodegradation and biodegradability---Explanations and limits |
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4 | (6) |
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4 Current plastic market and production |
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10 | (7) |
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17 | (1) |
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6 Plastic waste and disposal |
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18 | (2) |
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7 Mismanagement of plastic disposal and recycling---Plastic littering |
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20 | (1) |
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8 Political actions against plastic littering |
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21 | (1) |
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9 Conclusion and future development |
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22 | (2) |
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24 | (1) |
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24 | (3) |
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2 Methods for microplastic sampling and analysis in the seawater and fresh water environment |
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27 | (20) |
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28 | (2) |
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2 Water sample collection methods |
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30 | (8) |
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3 Sample pretreatment before identification |
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38 | (3) |
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41 | (1) |
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41 | (1) |
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6 Blank and contamination control |
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42 | (1) |
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42 | (1) |
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43 | (1) |
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43 | (4) |
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3 Exploring microbial consortia from various environments for plastic degradation |
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47 | (24) |
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Ingrid Eileen Meyer Cifuentes |
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48 | (1) |
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2 Enrichment and cultivation of aerobic plastic-degrading consortia |
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49 | (7) |
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3 Enrichment and cultivation of anaerobic plastic-degrading consortia |
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56 | (9) |
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65 | (1) |
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65 | (6) |
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4 Cultivation of filamentous fungi for attack on synthetic polymers via biological Fenton chemistry |
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71 | (24) |
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72 | (7) |
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2 Rationale for the cultivation of fungi for Fenton chemistry-dependent attack on synthetic polymers |
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79 | (1) |
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3 Materials, equipment and reagents |
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80 | (2) |
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82 | (5) |
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87 | (1) |
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6 Applicable analytical methods to detect fungal effects on polymers |
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87 | (4) |
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91 | (1) |
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92 | (1) |
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92 | (3) |
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5 Characterization of biodegradation of plastics in insect larvae |
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95 | (26) |
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96 | (1) |
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2 Biodegradation of plastics in Tenebrio molitor |
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97 | (3) |
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3 Analytical methods for plastic biodegradation |
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100 | (3) |
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4 Protocols for the characterization of plastic degradation by T. molitor larvae |
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103 | (9) |
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112 | (6) |
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118 | (1) |
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119 | (1) |
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119 | (2) |
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6 Quantification of polystyrene plastics degradation using 14C isotope tracer technique |
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121 | (16) |
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122 | (1) |
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123 | (11) |
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134 | (1) |
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134 | (1) |
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135 | (1) |
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135 | (2) |
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7 Exploring the global metagenome for plastic-degrading enzymes |
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137 | (22) |
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138 | (6) |
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144 | (1) |
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145 | (6) |
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151 | (1) |
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152 | (1) |
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152 | (7) |
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8 Cutinases from thermophilic bacteria (actinomycetes): From identification to functional and structural characterization |
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159 | (28) |
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160 | (1) |
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2 Identification of thermophilic cutinases |
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161 | (1) |
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3 Structural and thermodynamic analysis of PET-hydrolyzing cutinases |
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162 | (7) |
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169 | (13) |
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182 | (1) |
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182 | (1) |
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182 | (5) |
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9 Ideonella sakaiensis, PETase, and MHETase: From identification of microbial PET degradation to enzyme characterization |
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187 | (20) |
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188 | (4) |
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2 Screening microorganisms that degrade PET and isolation of microbial consortium no. 46 |
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192 | (1) |
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3 Isolation of I. sakaiensis 201-F6 from microbial consortium no. 46 |
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193 | (2) |
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4 Detection of microbial PET degradation |
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195 | (2) |
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5 Characterization of PETase and MHETase |
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197 | (5) |
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202 | (1) |
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203 | (1) |
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203 | (1) |
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203 | (4) |
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10 GRAPE, a greedy accumulated strategy for computational protein engineering |
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207 | (24) |
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208 | (2) |
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210 | (1) |
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211 | (15) |
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226 | (2) |
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228 | (1) |
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229 | (2) |
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11 Mechanistic investigation of enzymatic degradation of polyethylene terephthalate by nuclear magnetic resonance |
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231 | (22) |
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232 | (1) |
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2 `H solution NMR analysis to quantify PET chain scissions |
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233 | (3) |
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3 Solid-state NMR analysis to determine PET chain conformation and dynamics |
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236 | (12) |
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248 | (1) |
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249 | (1) |
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249 | (1) |
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249 | (4) |
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12 Fluorimetric high-throughput screening method for polyester hydrolase activity using polyethylene terephthalate nanoparticles |
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253 | (18) |
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Christoffel P.S. Badenhorst |
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254 | (1) |
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2 PET nanoparticles: Generation, application, and characterization |
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255 | (3) |
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3 Fluorimetric high-throughput screening assay |
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258 | (3) |
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4 Materials, equipment, and reagents |
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261 | (3) |
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264 | (4) |
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268 | (1) |
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268 | (3) |
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13 Anchor peptides promote degradation of mixed plastics for recycling |
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271 | (22) |
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272 | (2) |
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2 Engineering the adhesion peptide binding modules for enhanced polymer absorption and enzymatic degradation |
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274 | (3) |
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277 | (1) |
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278 | (2) |
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280 | (8) |
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288 | (1) |
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288 | (1) |
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288 | (5) |
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14 Tuning of adsorption of enzymes to polymer |
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293 | (24) |
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294 | (3) |
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2 Expression and purification of polymer degrading hydrolases |
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297 | (3) |
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3 Surface engineering by site-directed mutagenesis |
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300 | (3) |
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4 Fusion of hydrophobic binding domains |
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303 | (5) |
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5 Enzyme truncation and mutagenesis of the metal-binding site |
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308 | (4) |
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312 | (1) |
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313 | (4) |
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15 Characterization of the enzymatic degradation of polyurethanes |
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317 | (20) |
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318 | (4) |
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2 Materials, equipment and reagents |
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322 | (2) |
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324 | (5) |
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4 Safety considerations and standards |
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329 | (1) |
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5 Analysis and statistics |
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329 | (4) |
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333 | (1) |
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7 Alternative methods/procedures |
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334 | (1) |
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8 Troubleshooting and optimization |
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334 | (1) |
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334 | (1) |
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335 | (1) |
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335 | (2) |
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16 Structural analysis of PET-degrading enzymes PETase and MHETase from Ideonella sakaiensis |
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337 | (20) |
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338 | (2) |
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340 | (2) |
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3 Expression and purification |
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342 | (4) |
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346 | (6) |
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5 Modeling of a PETase-BHET complex |
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352 | (2) |
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354 | (1) |
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355 | (1) |
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355 | (2) |
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17 Structural and functional characterization of nylon hydrolases |
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357 | (34) |
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358 | (3) |
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2 Screening of microorganisms and enzymes degrading nylon-related compounds |
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361 | (1) |
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3 Preparation of oligomeric and polymeric substrates for enzyme assays |
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361 | (5) |
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4 Enzymatic hydrolysis of nylons and related substrates |
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366 | (3) |
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5 Structural analysis of 6-aminohexanoate dimer hydrolase NyIB |
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369 | (3) |
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372 | (14) |
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386 | (1) |
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387 | (1) |
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387 | (4) |
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18 Upcycling of hydrolyzed PET by microbial conversion to a fatty acid derivative |
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391 | (32) |
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392 | (5) |
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397 | (18) |
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415 | (1) |
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416 | (1) |
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416 | (7) |
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19 Screening and cultivating microbial strains able to grow on building blocks of polyurethane |
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423 | (12) |
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Maria Jose Cardenas Espinosa |
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424 | (2) |
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426 | (6) |
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432 | (1) |
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433 | (1) |
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433 | (2) |
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20 Engineering microalgae as a whole cell catalyst for PET degradation |
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435 | (22) |
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436 | (2) |
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2 Microalgae as phototrophic cell factories for recombinant protein production |
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438 | (6) |
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3 Introducing the PETase gene into the genome of a diatom |
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444 | (2) |
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446 | (4) |
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5 Analysis of enzymatic plastic degradation |
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450 | (3) |
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453 | (1) |
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453 | (1) |
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453 | (4) |
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21 Yeast cell surface display of bacterial PET hydrolase as a sustainable biocatalyst for the degradation of polyethylene terephthalate |
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457 | |
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458 | (7) |
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465 | (8) |
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473 | (1) |
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473 | |
