Focusing on the scientific understanding of enzyme functionality and protein design for the creation of altered characteristics for new enzyme variants, this book presents background concepts, then outlines the structural knowledge needed to guide random mutagenesis to selected areas of the protein, and describes methods for creating and selecting variant proteins with the desired characteristics. Chapters are organized in sections on enzyme design, enzyme diversity generation, and screening. Within these areas, the book focuses on ideas, function, and results rather than on precise method description. Svendsen is senior science manager of protein design in the Danish private-sector company. Annotation ©2004 Book News, Inc., Portland, OR (booknews.com)
Enzyme Functionality serves as a conduit for trailblazing research in enzyme engineering-relating current understanding of sequence families, the new notion of enzyme structure classes, and modern methods in protein engineering, design, and directed evolution to accelerate the development of novel enzyme functionalities. This reference gathers the diverse perspectives of nearly 80 scientists from around the globe and surveys all leading rational and random approaches to the artificial evolution of enzymes. Citing more than 1500 notable works, it outlines assays for enzyme activity, stability, and specificity and a wide variety of site-directed, redesign, and evolutionary engineering methods.
