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Essential Zebrafish Methods: Cell and Developmental Biology [Minkštas viršelis]

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Edited by (Professor of Biochemistry and Marine Biology at Northeastern), Edited by (Howard Hughes Medical Institute, Children's Hospital, Boston, MA, USA), Edited by (Professor, Department of Biology, Institute of Neuroscience, University of Oregon, Eugene, OR, USA)
  • Formatas: Paperback / softback, 584 pages, aukštis x plotis: 235x191 mm, weight: 970 g
  • Serija: Reliable Lab Solutions
  • Išleidimo metai: 09-Oct-2009
  • Leidėjas: Academic Press Inc
  • ISBN-10: 0123745993
  • ISBN-13: 9780123745996
Kitos knygos pagal šią temą:
Essential Zebrafish Methods: Cell and Developmental BiologyDidesnis paveikslėlis
  • Formatas: Paperback / softback, 584 pages, aukštis x plotis: 235x191 mm, weight: 970 g
  • Serija: Reliable Lab Solutions
  • Išleidimo metai: 09-Oct-2009
  • Leidėjas: Academic Press Inc
  • ISBN-10: 0123745993
  • ISBN-13: 9780123745996
Kitos knygos pagal šią temą:
Pastovi nuoroda: https://www.kriso.lt/db/9780123745996.html
Raktažodžiai:
Due to its prolific reproduction and the external development of the transparent embryo, the zebrafish is the prime model for genetic and developmental studies, as well as research in genomics. While genetically distant from humans, nonetheless the vertebrate zebrafish has comparable organs and tissues that make it the model organism for study of vertebrate development. This book, one of two new volumes in the Reliable Lab Solutions series dealing with zebrafish, brings together a robust and up-to-date collection of time-tested methods presented by the worlds leading scientists. Culled from previously published chapters in Methods in Cell Biology and updated by the original authors where relevant, it provides a comprehensive collection of protocols describing the most widely used techniques relevant to the study of the cellular and developmental biology of zebrafish. The methods in this volume were hand-selected by the editors, whose goal was to a provide a handy and cost-effective collection of fail-safe methods, tips, and tricks of the trade” to both experienced researchers and more junior members in the lab.

Daugiau informacijos

The best techniques and practices for researchers using zebrafish as a research model as compiled by the editors of the well-known Methods in Cell Biology series.
Contributors xiii
Preface xvii
Overview of the Zebrafish System
H. William Detrich
Monte Westerfield
Leonard I. Zon
Introduction
1(1)
History of the Zebrafish System and Its Advantages and Disadvantages
2(2)
Cell and Developmental Biology, Organogenesis, and Human Disease
4(1)
Genetics and Genomics
5(1)
Future Prospects
6(1)
Conclusions
6(1)
Epilogue: Volumes 76 and 77 and Technological Advances to Come
7(4)
References
7(4)
Cell Cycles and Development in the Embryonic Zebrafish
Donald A. Kane
Introduction
11(1)
Terminology and the Staging Series
12(1)
The Zygote Period
12(2)
The Cleavage Period
14(2)
The Blastula Period
16(4)
The Gastrula Period
20(5)
The Segmentation Period
25(4)
References
26(3)
Primary Fibroblast Cell Culture
Barry H. Paw
Leonard I. Zon
Introduction
29(1)
Material and Methods
30(2)
Results and Discussion
32(4)
References
33(3)
Production of Haploid and Diploid Androgenetic Zebrafish (Including Methodology for Delayed In Vitro Fertilization)
Graham E. Corley-Smith
Bruce P. Brandhorst
Charline Walker
John H. Postlethwait
Introduction
36(2)
Equipment and Materials
38(2)
Methods
40(4)
Results and Discussion
44(4)
Conclusions and Perspectives
48(4)
References
49(3)
Analysis of Protein and Gene Expression
Trevor Jowett
In Situ Hybridization to RNA and Immunolocalization of Proteins
52(1)
Probe Synthesis
53(2)
Fixation
55(1)
Hybridization to Whole-Mount Embryos
56(3)
Immunolocalization of Probes
59(2)
Two-Color In Situ Hybridizations
61(4)
Double-Fluorescent In Situ Hybridization
65(1)
Simultaneous Localization of Transcription and Translation Gene Products
66(4)
Embedding and Sectioning Whole-Mount Embryos
70(1)
Solutions and Reagents
70(4)
References
72(2)
Analysis of Zebrafish Development Using Explant Culture Assays
Yevgenya Grinblat
Hazel Sive
Introduction
74(3)
Zebrafish Explants: General Considerations
77(1)
Materials Required
78(8)
Guide to Explant Isolation and Culture
86(8)
Using Explants to Assay Induction
94(3)
Illustrations of Specification and Induction Assays
97(3)
Future Directions
100(4)
References
100(4)
Confocal Microscopic Analysis of Morphogenetic Movements
Mark S. Cooper
Leonard A. D'Amico
Clarissa A. Henry
Introduction
104(1)
Confocal Imaging of Embryos
105(1)
General Principles of Vital Staining
105(11)
Mounting Embryos for Imaging
116(2)
Imaging Procedures
118(2)
Multilevel Time-Lapse Confocal Analysis
120(7)
Distribution of Visual Information
127(2)
Confocal Imaging of Embryos Expressing Green Fluorescent Protein (GFP)
129(1)
Summary
130(4)
References
131(3)
Cytoskeletal Dynamics of the Zebrafish Embryo
Jacek Topczewski
Lilianna Solnica-Krezel
Introduction
134(1)
Cytoskeleton of the Unfertilized Egg
135(1)
Organization and Function of the Cytoskeleton in the Zygote
136(4)
Cleavage and Blastula Period
140(2)
Yolk Cell Microtubules during Epiboly
142(4)
Tubulin Dynamics in Neuronal Axons of Living Zebrafish Embryos
146(1)
Intermediate Filaments in Zebrafish
146(1)
Methods
147(12)
References
154(5)
Analyzing Axon Guidance in the Zebrafish Retinotectal System
Lara D. Hutson
Douglas S. Campbell
Chi-Bin Chien
Introduction
159(1)
Retinotectal Mutants
160(3)
Labeling the Retinotectal System
163(11)
Perturbing the Retinotectal System
174(4)
Future Directions
178(5)
References
179(4)
Analysis of Cell Proliferation, Senescence, and Cell Death in Zebrafish Embryos
Daniel Verduzco
James F. Amatruda
Introduction: The Cell Cycle in Zebrafish
183(3)
Zebrafish Embryo Cell-Cycle Protocols
186(10)
Screening for Chemical Suppressors of Zebrafish Cell-Cycle Mutants
196(3)
Conclusions
199(1)
Reagents and Supplies
199(6)
References
200(5)
Cellular Dissection of Zebrafish Hematopoiesis
David L. Stachura
David Traver
Introduction
205(1)
Zebrafish Hematopoiesis
206(9)
Hematopoietic Cell Transplantation
215(8)
Enrichment of HSCs
223(3)
In Vitro Culture of Hematopoietic Progenitors
226(2)
Conclusions
228(6)
References
229(5)
Culture of Embryonic Stem and Primordial Germ Cell Lines from Zebrafish
Ten-Tsao Wong
Lianchun Fan
Paul Collodi
Introduction
234(1)
Methods
235(6)
Materials
241(4)
References
243(2)
Neurogenesis
Prisca Chapouton
Marion Coolen
Laure Bally-Cuif
Introduction
245(1)
The Primary Neuronal Scaffold
246(3)
Early Development of the Zebrafish Neural Plate
249(7)
Lateral Inhibition and the Neurogenesis Cascade
256(5)
Establishment of Neuronal Identity
261(1)
Lab Methods to Study Adult Neurogenesis
262(3)
Useful Tools for the Study of Zebrafish Neurogenesis
265(1)
Conclusion
265(29)
References
276(18)
Time-Lapse Microscopy of Brain Development
Reinhard W. Koster
Scott E. Fraser
Introduction: Why and When to Use Intravital Imaging
294(1)
Techniques for Vital Staining of the Nervous System
295(10)
Preparation of the Zebrafish Specimen
305(3)
The Microscopic System
308(2)
Data Recording
310(2)
Data Analysis
312(4)
Pitfalls to Avoid
316(2)
Summary
318(7)
References
319(6)
Development of the Peripheral Sympathetic Nervous System in Zebrafish
Rodney A. Stewart
A. Thomas Look
John P. Kanki
Paul D. Henion
Introduction
325(1)
The Peripheral Autonomic Nervous System
326(4)
The Zebrafish as a Model System for Studying PSNS Development
330(12)
Zebrafish as a Novel Model for Studying Neuroblastoma
342(1)
Conclusion and Future Directions
343(6)
References
344(5)
Approaches to Study the Zebrafish Retina
Jarema Malicki
Andrei Avanesov
Introduction
349(2)
Development of the Zebrafish Retina
351(8)
Analysis of Wild-Type and Mutant Visual System
359(19)
Analysis of Gene Function in the Zebrafish Retina
378(10)
Summary
388(14)
References
388(14)
Instrumentation for Measuring Oculomotor Performance and Plasticity in Larval Organisms
James C. Beck
Edwin Gilland
Robert Baker
David W. Tank
Introduction
402(3)
Methods
405(7)
Results and Discussion
412(13)
Conclusion
425(4)
References
426(3)
Development of Cartilage and Bone
Yashar Javidan
Courtney Alexander
Thomas F. Schilling
Introduction
429(9)
Cartilage Visualization Techniques
438(6)
Bone Visualization Techniques
444(4)
Molecular Markers and Transgenic Lines
448(2)
Strategy and Potential of Future Screens for Skeletal Mutants
450(7)
References
451(6)
Morphogenesis of the Jaw: Development Beyond the Embryo
R. Craig Albertson
Pamela C. Yelick
Larval Zebrafish Craniofacial Cartilage Development
457(2)
Analysis of Craniofacial Skeletal and Replacement Tooth Development
459(12)
Conclusion
471(1)
Updates and Recent Advances
472(7)
References
474(5)
Cardiac Development
Le A. Trinh
Didier Y. R. Stainier
Update
479(1)
Introduction
480(1)
Stages of Heart Tube Morphogenesis
480(11)
Gene Expression
491(4)
Conclusion and Future Directions
495(6)
References
496(5)
Zebrafish Kidney Development
Iain A. Drummond
Introduction
501(1)
Pronephric Structure and Function
502(2)
Pronephric Development
504(18)
The Zebrafish Pronephros as a Model of Human Disease
522(3)
Conclusions
525(10)
References
526(9)
Index 535
Professor, Department of Biology, Institute of Neuroscience, University of Oregon, Eugene, OR, USA Professor of Biochemistry and Marine Biology at Northeastern University, promoted 1996. Joined Northeastern faculty in 1987. Previously a faculty member in Dept. of Biochemistry at the University of Mississippi Medical Center, 1983-1987.Principal Investigator in the U.S. Antarctic Program since 1984. Twelve field seasons "on the ice" since 1981. Research conducted at Palmer Station, Antarctica, and McMurdo Station, Antarctica.Research areas: Biochemical, cellular, and physiological adaptation to low and high temperatures. Structure and function of cytoplasmic microtubules and microtubule-dependent motors from cold-adapted Antarctic fishes. Regulation of tubulin and globin gene expression in zebrafish and Antarctic fishes. Role of microtubules in morphogenesis of the zebrafish embryo. Developmental hemapoiesis in zebrafish and Antarctic fishes. UV-induced DNA damage and repair in Antarctic marine organisms.