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El. knyga: Essentials in Modern HPLC Separations

(Senior Principal Scientist, RJ Reynolds Tobacco Co., Winston-Salem, NC, USA), (Professor and Head of the Department of Analytical Chemistry, University of Bucharest, Romania)
  • Formatas: EPUB+DRM
  • Išleidimo metai: 24-Jun-2022
  • Leidėjas: Elsevier - Health Sciences Division
  • Kalba: eng
  • ISBN-13: 9780323998369
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Essentials in Modern HPLC SeparationsDidesnis paveikslėlis
  • Formatas: EPUB+DRM
  • Išleidimo metai: 24-Jun-2022
  • Leidėjas: Elsevier - Health Sciences Division
  • Kalba: eng
  • ISBN-13: 9780323998369
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Essentials in Modern HPLC Separations, Second Edition discusses the role of separation in high performance liquid chromatography (HPLC). This new and updated edition systematically presents basic concepts as well as new developments in HPLC. Starting with a description of basic concepts, it provides important guidance for the practical utilization of various HPLC procedures, such as the selection of the HPLC type, proper choice of the chromatographic column, selection of mobile phase and selection of the method of detection, all of which are in correlation with the physico-chemical characteristics of the compounds separated.

Every chapter has been carefully reviewed, with several new sections added to bring the book completely up-to-date. Hence, it is a valuable reference for students and professors in chemistry.

  • Provides a thoroughly updated resource, with an entirely new section on Computer-aided Method Development in HPLC and new subsections on miniaturization and automation in HPLC, chemometric aspects of HPLC, green solvent use in HPLC, and more
  • Includes insights into the chromatographic process to find the optimum solution for analyzing complex samples
  • Presents a basis for understanding the utilization of modern HPLC for applications, particularly for the analysis of pharmaceutical, biological, food, beverage and environmental samples

Part
1. Basic information about HPLC1. Introductory information regarding HPLC
2. Overview of HPLC instrumentation and its use3. Parameters for the characterization of HPLC separation4. Equilibrium types in HPLC5. Intermolecular forces6. Characterization of analytes and matrices
7. Mobile phases and their properties8. Analytical HPLC columns and their characteristics

Part
2. Main types of HPLC separations 9. Reversed-phase HPLC10. Other HPLC separations performed on hydrophobic stationary phases11. Hydrophilic interaction chromatography (HILIC)12. Other HPLC separations performed on polar stationary phases13. Ion-exchange, ion-moderated, and ligand-exchange liquid chromatography14. Chiral HPLC Separations15. Size exclusion HPLC16. Affinity, immunoaffinity, and aptamer type HPLC17. Mixed mode HPLC

Part
3. Practice of HPLC analysis18. Utilization of HPLC in chemical analysis

Part
4. Appendices

Serban C. Moldoveanu was Senior Principal Scientist at R. J. Reynolds Tobacco Company, USA, and retired at the end of 2023. His research activity was focused on various aspects of chromatography including method development for the analysis by GC/MS, HPLC, and LC/MS/MS of natural products and cigarette smoke. He has also performed research on pyrolysis of a variety of polymers and small molecules. He has written over 150 publications in peer reviewed journals, eleven books, and several chapter contributions. He is a member of the editorial board of the Journal of Analytical Methods in Chemistry and of Frontiers in Chemistry.

Victor David was a Professor and Head of the Department of Analytical Chemistry, University of Bucharest, Romania. Recently retired, he remains Emeritus Professor at the same Department. His main field of research is separation science (theory and applications in various scientific domains). He is the author of more than 200 publications, including over 150 scientific papers in peer reviewed journals and 25 books and chapters. He is member of editorial board of Biomedical Chromatography, Molecules, Journal of Essential Oil-Bearing Plants and Revue Roumaine de Chimie.
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