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El. knyga: Experimental Surgical Models in the Laboratory Rat

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  • Formatas: 232 pages
  • Išleidimo metai: 19-Apr-2016
  • Leidėjas: CRC Press Inc
  • Kalba: eng
  • ISBN-13: 9781420093278
Kitos knygos pagal šią temą:
  • Formatas: 232 pages
  • Išleidimo metai: 19-Apr-2016
  • Leidėjas: CRC Press Inc
  • Kalba: eng
  • ISBN-13: 9781420093278
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An All-Inclusive Guide to Surgical Techniques on Rats

The design of an adequate surgical model, like the choice of the animal model itself, is extremely important for obtaining reliable valuable data. Experimental Surgical Models in the Laboratory Rat summarizes a series of techniques that were applied in the Bone Biology Laboratories, School of Medicine, Rosario National University, Argentina.

Stopping just short of an exhaustive analysis of the techniques, this manual describes the basics of each surgical technique, the necessary materials, precautions to consider, the methodology to apply, and the possible results to be obtained in similar experiments.

CD-ROM: 250 Illustrations of Surgery Techniques with Step-by-Step Instruction

With succinct descriptions, color photographs, and easy-to-follow steps, the downloadable resources are a practical, full-color roadmap for performing various surgery techniques, such as catheterization, tracheostomy, intramuscular injection, subcutaneous injection, intraperitoneal injection, and oral intubation. This definitive work provides technical and practical data including volumes and concentrations of solutions, catheters used, equipment, and biological variables on the rat. Experimental Surgical Models in the Laboratory Rat is a complete reference for todays biomedical sciences investigator.
Preface xi
Acknowledgments xiii
The Editors xv
Contributors xvii
Abbreviations xix
SECTION I Introduction
Bioethics and Animal Care
3(6)
Lucas R.M. Brun
Veronica E. Di Loreto
Control of Stress and Distress
9(8)
Maria F. Landoni
Management of Pain in Laboratory Animals
17(4)
Maria F. Landoni
Anesthesia and Analgesia
21(10)
Lucas F. de Candia
Alfredo Rigalli
Veronica E. Di Loreto
Euthanasia
31(2)
Alfredo Rigalli
Antibiotic
33(4)
Veronica E. Di Loreto
Alfredo Rigalli
SECTION II General Procedures
Operating Theatre
37(4)
Veronica E. Di Loreto
Alfredo Rigalli
Rat Identification
41(2)
Veronica E. Di Loreto
Alfredo Rigalli
Antisepsis, Sterilization, and Asepsis
43(4)
Diego Holotte
Jesica Nipoti
Maria J. Pretini
Veronica E. Di Loreto
Suture
47(8)
Aneley Traverso
Alfredo Rigalli
Substances Administration
55(8)
Maela Lupo
Veronica E. Di Loreto
Alfredo Rigalli
Samples
63(10)
Veronica E. Di Loreto
Laura I. Pera
Alfredo Rigalli
SECTION III Catheterization and Cannulation
Catheterization of the Femoral Artery and Vein
73(4)
Veronica E. Di Loreto
Alfredo Rigalli
Cardiac Catheterization
77(6)
Cristina Lorenzo Carrion
Laura Krieger
Manuel Rodriguez
Martin Donato
Cannulation of the Thoracic Duct
83(4)
Gabriel A. Inchauspe
Alfredo Rigalli
Tracheostomy
87(4)
Laura I. Pera
Alfredo Rigalli
SECTION IV Gastrointestinal Tract
In Situ Isolation of the Stomach
91(4)
Alfredo Rigalli
In Situ Isolation of the Intestinal Loop
95(4)
Maria L. Brance
Lucas R.M. Brun
Alfredo Rigalli
Intestinal Everted Sacs
99(4)
Lucas R.M. Brun
Maria L. Brance
Lucas F. de Candia
Alfredo Rigalli
In Situ Perfusion of the Small Intestine
103(6)
Alfredo Rigalli
SECTION V Pancreas
Experimental Models for the Study of Diabetes
109(6)
Veronica E. Di Loreto
Alfredo Rigalli
Islets of Langerhans Isolation
115(2)
Alfredo Rigalli
Incubation of Pancreatic Tissue Slices
117(4)
Ines Menoyo
Alfredo Rigalli
In Situ Perfusion of the Pancreas
121(4)
Ines Menoyo
Alfredo Rigalli
Experimental Pancreatitis
125(10)
Diego Holotte
Jesica Nipoti
Maria J. Pretini
Veronica E. Di Loreto
Alfredo Rigalli
SECTION VI Liver
Hepatic Circulation Impairment
135(4)
Gabriel A. Inchauspe
Alfredo Rigalli
Extrahepatic Cholestasis Model
139(6)
Anabel Brandoni
Adriana M. Torres
SECTION VII Ablation of Endocrine Glands
Adrenalectomy
145(4)
Laura I. Pera
Alfredo Rigalli
Ovariectomy
149(4)
Veronica E. Di Loreto
Laura I. Pera
Alfredo Rigalli
Thyroparathyroidectomy
153(4)
Veronica E. Di Loreto
Alfredo Rigalli
Parathyroid Glands
157(4)
Maela Lupo
Alfredo Rigalli
SECTION VIII Kidneys
Measurement Techniques of Renal Parameters
161(8)
Veronica E. Di Loreto
Alfredo Rigalli
Kidney Isolation and Perfusion
169(8)
Veronica E. Di Loreto
Alfredo Rigalli
Acute Renal Failure Models
177(6)
Gisela Di Giusto
Adriana M. Torres
Renal Blood Flow Measurement
183(6)
Gisela Di Giusto
Adriana M. Torres
Alfredo Rigalli
Transport Studies in Plasma Membrane Vesicles Isolated from Renal Cortex
189(6)
Gisela Di Giusto
Adriana M. Torres
Remnant Kidney Model
195(6)
Maria V. Arcidiacono
Luis A. Ramirez
Valeria Dalmau
Alfredo Rigalli
SECTION IX Circulatory System
Experimental Myocardial Infarction
201(4)
Ignacio M. Seropian
German E. Gonzalez
Experimental Arteriosclerosis
205(4)
Anabel Brandoni
Adriana M. Torres
Isolated Heart: Langendorff Technique
209(8)
Bruno Buchholz
Veronica D'Annunzio
Ricardo J. Gelpi
SECTION X Additional Techniques
Polyclonal Antibodies Preparation
217(4)
Laura I. Pera
Lucas R.M. Brun
Alfredo Rigalli
Histological Procedures
221(8)
Maria L. Brance
Lucas R.M. Brun
Bones and Bone Tissue
229(6)
Hilda Moreno
Mercedes Lombarte
Veronica E. Di Loreto
SECTION XI Miscellaneous
Equipment
235(6)
Alfredo Rigalli
Solution Preparation
241(2)
Veronica E. Di Loreto
Alfredo Rigalli
Normal Values in IIm/Fm Rat Subline ``M''
243(4)
Veronica E. Di Loreto
Alfredo Rigalli
Index 247
Alfredo Rigalli, Veronica Di Loreto