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Functional Imaging by Controlled Nonlinear Optical Phenomena [Kietas viršelis]

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This book gives an overview of the nonlinear optical process by ultrafast laser pulses and explains how the basics of nonlinear optical microscopy led to the most advanced techniques of photo-controlled nonlinear optical microscopy--Provided by publisher. Ultrafast lasers allow high-precision imaging and manipulation for biological and medical applications. Nonlinear optical microscopy has provided researchers with unique possibilities of three-dimensional imaging of biological cells and tissues. Nonlinear optical imaging technique is a rapidly emerging research area with widespread fundamental research and clinical applications. Nonlinear optical imaging allows both structural and functional imaging with cellular level resolution imaging in biological systems. The introduction of endogenous or exogenous probes can selectively enhance contrast for molecular targets in a living cell as well as supply functional information on processes. With the aim to control nonlinear optical processes and to obtain functional images, nonlinear optical processes can be controlled by photo-controlled probes and/or parameters of ultrafast laser pulses, such as time, space, polarization, and phase.This book gives an overview of the nonlinear optical process by ultrafast laser pulses and explains how the basics of nonlinear optical microscopy led to the most advanced techniques of photo-controlled nonlinear optical microscopy.

Recenzijos

The authors have provided a remarkable reference on nonlinear optical and functional imaging, which includes powerful microscopy and spectroscopy techniques via ultrafast laser pulses ... Researchers and graduate students interested in microscopy, spectroscopy and nonlinear optics would benefit from this book. (Optics & Photonics News, 4 April 2014)

List Of Figures ix
List Of Tables xxi
Preface xxiii
Acknowledgments xxv
Acronyms xxvii
1 Ultrafast Optics For Nonlinear Optical Microscopy 1(86)
1.1 Nonlinear Optical Phenomena
1(53)
1.1.1 Introduction to Nonlinear Optics
1(11)
1.1.2 Second-Order Nonlinear Optical Phenomena
12(13)
1.1.3 Third-Order Nonlinear Optical Phenomena
25(29)
1.2 Nonlinear Ionization
54(4)
1.2.1 Nonlinear Optical Ionization
54(1)
1.2.2 Avalanche Ionization
55(1)
1.2.3 Photodisruption/Optical Breakdown
56(2)
1.3 Light Source
58(22)
1.3.1 Ultrashort Laser Pulse
58(2)
1.3.2 Dispersion Management and Pulse Shaping
60(12)
1.3.3 Pulse Characterization in Nonlinear Optical Microscope
72(6)
1.3.4 Wavefront Compensation
78(2)
References
80(7)
2 Basic Microscopic Technique 87(58)
2.1 Basic Architecture of a Laser Scanning Microscope
87(4)
2.1.1 Scanning Methods
89(1)
2.1.2 Signal Detection
90(1)
2.2 Fluorescence Technique
91(39)
2.2.1 Various Fluorescent Molecules and Multi-Color Fluorescence Imaging
191
2.2.2 Fluorescence Resonance Energy Transfer Imaging
101(6)
2.2.3 Fluorescence Lifetime-Resolved Imaging
107(9)
2.2.4 Fluorescence Recovery after Photobleaching Imaging
116(5)
2.2.5 Fluorescence Correlation Spectroscopy
121(9)
References
130(15)
3 Nonlinear Optical Spectroscopy (NLOS) 145(28)
3.1 Laser-Wavelength Scanning Method
145(4)
3.2 Multiplex Spectroscopy
149(9)
3.2.1 Principle of Multiplex Nonlinear Optical Spectroscopy
150(7)
3.2.2 Experimental Setup for Multiplex-NLOS
157(1)
3.2.3 Measurement of Spectroscopic Information by Multiplex-NLOS
157(1)
3.3 Fourier-Transform Spectroscopy
158(11)
3.3.1 Principle of FT-NLOS
160(7)
3.3.2 Experimental Setup for FT-NLOS
167(1)
3.3.3 Measurement of Broadband Excitation Spectra by FT-NLOS
167(2)
References
169(4)
4 Nonlinear Optical Microscopy 173(94)
4.1 Introduction to Nonlinear Optical Microscopy
173(3)
4.1.1 Various Nonlinear Optical Microscopies
173(1)
4.1.2 Basic Architecture of a Nonlinear Optical Microscope
174(2)
4.1.3 Spatial Resolution
176(1)
4.2 Fluorescence Imaging
176(28)
4.2.1 Basic Two-Photon Excited Fluorescence (TPEF) Microscopy
176(8)
4.2.2 Application of TPEF Microscopy
184(6)
4.2.3 High-Speed Imaging Using TPEF Microscopy
190(14)
4.3 Electronic Resonance Imaging
204(10)
4.3.1 Stimulated Parametric Emission Microscopy
204(7)
4.3.2 Two-Photon Absorption Microscopy
211(3)
4.4 Vibrational Imaging
214(20)
4.4.1 Coherent Anti-Stokes Raman Scattering Microscopy
214(16)
4.4.2 Stimulated Raman Scattering Microscopy
230(4)
4.5 Second-Harmonic Generation Imaging
234(10)
4.6 Refractive Index Imaging
244(8)
4.6.1 Third-Harmonic Generation Microscopy
244(5)
4.6.2 Nonresonant Four-Wave Mixing Microscopy
249(3)
References
252(15)
5 Functional Imaging Based On Molecular Control 267(60)
5.1 Localized Optical Marking and Tracking Using Photomodulatable Fluorescent Molecules
267(12)
5.1.1 Photomodulatable Fluorescent Molecules
268(7)
5.1.2 Imaging with Localized Optical Manipulation of Photosensitive Molecules
275(4)
5.2 Multifarious Control of Multiphoton Excitation by Pulse-Shaping Technique
279(19)
5.2.1 Control of Two-Photon Fluorescence in Multi-Labeled Sample
279(10)
5.2.2 Control of Vibrational Mode Excitation by a Single Broadband Pulse
289(9)
5.3 Super-Resolution Imaging Utilizing Nonlinear Response
298(14)
5.3.1 Stimulated Depletion Emission Microscopy
299(4)
5.3.2 Saturated Excitation Microscopy
303(4)
5.3.3 Saturated Structured Illumination Microscopy
307(3)
5.3.4 Single-Molecule Localization Microscopy
310(2)
References
312(15)
6 Ultrafast Laser Surgery 327(16)
6.1 Laser Cell Nanosurgery
327(8)
6.1.1 Femtosecond Laser Surgery
327(8)
6.1.2 Plasmonic-Enhanced Nanosurgery
335(1)
6.2 Photodisruption and Photo-Stimulation
335(1)
6.2.1 Photodisruption of Tissues
335(1)
6.2.2 Laser-Induced Stimulation
336(1)
References
336(7)
Index 343
KEISUKE ISOBE, DEng, is a Research Scientist at the RIKEN Center for Advanced Photonics. WATARU WATANABE, DEng, is a Professor in the Department of Electrical & Electronic Engineering, College of Science and Engineering, at Ritsumeikan University. KAZUYOSHI ITOH, DEng, is a Professor Emeritus and Research Professor at the Science Technology Entrepreneurship Laboratory (E-Square), Osaka University.