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El. knyga: G Protein-Coupled Receptor Genetics: Research and Methods in the Post-Genomic Era

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G Protein-Coupled Receptor Genetics: Research and Methods in the Post-Genomic Era features practical techniques inspired by the fast moving GPCR field. From powerful bioinformatic tools tracing the evolution of GPCRs, to methods for the cellular transfection of engineered viruses containing GPCRs, to optogenetic techniques that produce light-activated GPCRs in live mice, what was once science fiction is now science fact. This detailed volume includes sections covering genetic mechanisms, a genetic toolbox for GPCR discovery, as well as genetic aspects of G protein-coupled receptors in health and medicine. Written for the Methods in Pharmacology and Toxicology series, this book contains the kind of key implementation advice that encourages successful results in the lab.

Authoritative and easy to use, G Protein-Coupled Receptor Genetics: Research and Methods in the Post-Genomic Era serves as an ideal guide for researchers aiming to continue our progress in this dynamic and exciting area of study.

Preface v
Contributors ix
PART I GENETIC MECHANISMS OF G PROTEIN-COUPLED RECEPTORS
1 G Protein-Coupled Receptors: Research and Methods in the Post-genomic Era
3(20)
Craig W. Stevens
2 Examining the Dynamic Evolution of G Protein-Coupled Receptors
23(22)
Claudia Staubert
Diana Le Duc
Torsten Schoneberg
3 Functional Properties of Virus-Encoded and Virus-Regulated G Protein-Coupled Receptor
45(22)
Katja Spiess
Mette M. Rosenkilde
4 Discovery and Function of the Very Large G Protein-Coupled Receptor
67(18)
Perrin C. White
D. Randy McMillan
5 Functional Evolution of Opioid Family G Protein-Coupled Receptors
85(20)
Eyal Vardy
Craig W. Stevens
Bryan L. Roth
6 Investigation of G Protein-Coupled Receptor Function and Regulation Using Antisense
105(22)
Jonathan Willets
Craig Nash
7 Signalosome Profiling Reveals Allosteric Interactions Between G Protein-Coupled Receptors
127(22)
Katrin Altosaar
Darlaine Petrin
Dominic Devost
Terence E. Hebert
PART II GENETIC TOOLBOX FOR G PROTEIN-COUPLED RECEPTOR DISCOVERY
8 Use of Optogenetic Approaches to Control Intracellular Signaling of G Protein-Coupled Receptors
149(12)
Olivia A. Masseck
Melanie D. Mark
Stefan Herlitze
9 Viral Infection for G Protein-Coupled Receptor Expression in Eukaryotic Cells
161(16)
Antonio Porcellini
Luisa Iacovelli
Antonio De Blasi
10 Using In Vitro Mutagenesis to Characterize Structure-Function Relationships in G Protein-Coupled Receptors
177(20)
Gregory W. Sawyer
Frederick J. Ehlert
11 The CRE Luc Mouse Model for Bioimaging Ligand Activation of G Protein-Coupled Receptors
197(22)
H. Gregory Polites
Kyriakos D. Economides
12 Inducing Conformational Changes in G Protein-Coupled Receptors by Domain Coupling
219(20)
Hamiyet Unal
Sadashiva S. Karnik
13 Rescue of Defective G Protein-Coupled Receptor Function by Intermolecular Cooperation
239(18)
Ashutosh Trehan
Kim Carol Jonas
Ilpo Huhtaniemi
Aylin Carla Hanyaloglu
Adolfo Rivero-Muller
14 Design of Super-arrestins for Gene Therapy of Diseases Associated with Excessive Signaling of G Protein-Coupled Receptors
257(32)
Vsevolod V. Gurevich
Eugenia V. Gurevich
PART III GENETIC ASPECTS OF G PROTEIN-COUPLED RECEPTORS IN HEALTH AND MEDICINE
15 Importance of G Protein-Coupled Receptor Genetics in Clinical Medicine
289(10)
Ramakrishna Devaki
16 Pharmacogenomics of G Protein-Coupled Receptor Signaling and Other Pathways in Essential Hypertension
299(14)
Pedro A. Jose
Van Anthony M. Villar
Matthew R. Weir
Robin A. Felder
17 Exon Sequencing of G Protein-Coupled Receptor Genes and Perspectives for Disease Treatment
313(20)
Angeliki Karamitri
Ralf Jockers
18 Gene Therapy Using G Protein-Coupled Receptors for the Treatment of Cardiovascular Disease
333(14)
Dario Leosco
Grazia D. Femminella
Claudio de Lucia
Giuseppe Rengo
19 Novel Mechanisms of G Protein-Coupled Receptor Oligomer and Ion Channel Interactions in Nociception
347(18)
Ajay S. Tekkirala
20 Discovery and Characterization of Leucine-Rich Repeat-Containing G Protein-Coupled Receptors
365(24)
Marie-Isabelle Garcia
Valeria Fernandez-Vallone
Gilbert Vassart
21 The Role of G-Protein-Coupled Receptors in Adult Neurogenesis
389(24)
Katie M. Collette
Van A. Doze
Dianne M. Perez
Index 413