Contributors |
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xi | |
Preface |
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xv | |
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Part 1 The Broader Issues |
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1 | (98) |
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1 Modern food biotechnology: overview of key issues |
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3 | (24) |
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3 | (2) |
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1.2 The basic techniques of genetic modification |
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5 | (2) |
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1.3 Genetic modification of agronomic traits in crops |
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7 | (1) |
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1.4 Environmental concerns |
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8 | (2) |
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1.5 The genetic modification of quality in foods |
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10 | (1) |
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1.6 The genetically modified tomato |
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11 | (3) |
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1.6.1 The need for a tomato with improved flavour |
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11 | (2) |
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1.6.2 The inhibition of pectin degradation in the GM tomato |
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1.6.3 Performance, safety and approval of the GM tomato |
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13 | (1) |
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1.6.4 The marketing of the GM tomato |
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14 | (1) |
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1.6.5 Prospects for quality improvement in fruit and vegetables |
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14 | (1) |
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1.7 Starter cultures for fermented foods |
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14 | (1) |
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1.8 Diagnostics for food testing |
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15 | (1) |
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1.9 The genetic modification of animals |
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16 | (2) |
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1.9.1 Herman the transgenic bull |
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17 | (1) |
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1.9.2 Dolly the cloned sheep |
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17 | (1) |
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17 | (1) |
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1.10 Safety and regulations |
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18 | (3) |
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20 | (1) |
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1.10.2 Patenting of life forms |
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20 | (1) |
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1.11 Consumer acceptance of gene technology in food production |
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21 | (1) |
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1.12 The case for communication |
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22 | (1) |
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1.13 The future of genetic modification in the food industry |
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23 | (4) |
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24 | (3) |
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2 Consumer perceptions of modern food biotechnology |
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27 | (20) |
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27 | (1) |
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2.2 Knowledge and public understanding |
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28 | (1) |
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2.3 The importance of social context |
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29 | (3) |
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2.4 The international picture |
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32 | (2) |
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2.5 Risk perception and consumer reaction |
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34 | (1) |
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2.6 Credibility of the risk regulators |
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35 | (1) |
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2.7 The role of the media in consumer acceptance |
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35 | (3) |
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2.8 The development of effective communication about biotechnology |
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38 | (5) |
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2.8.1 What should be communicated? |
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39 | (1) |
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40 | (1) |
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2.8.3 Prior attitudes and source characteristics |
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41 | (1) |
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2.8.4 Individual differences |
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42 | (1) |
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2.8.5 The consensus conference model |
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42 | (1) |
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43 | (1) |
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44 | (3) |
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44 | (3) |
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3 Moral concerns and the educational function of ethics |
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47 | (14) |
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3.1 Introduction - why bother about ethics? |
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47 | (1) |
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3.2 What are `moral and ethical concerns'? |
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48 | (2) |
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48 | (1) |
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49 | (1) |
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3.3 Why should there be moral concerns about food biotechnology? |
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50 | (1) |
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3.4 Is modern biotechnology wrong in itself? |
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51 | (4) |
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3.5 Do the possible consequences of modern biotechnology warrant moral concern? |
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55 | (3) |
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58 | (1) |
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59 | (2) |
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60 | (1) |
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60 | (1) |
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4 Worldwide regulatory issues: legislation and labelling |
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61 | (28) |
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61 | (1) |
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62 | (20) |
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62 | (3) |
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65 | (4) |
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69 | (5) |
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74 | (4) |
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78 | (2) |
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4.2.6 Australia and New Zealand |
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80 | (2) |
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82 | (1) |
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4.3 Moves towards international harmonisation |
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82 | (5) |
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4.3.1 Environmental legislation |
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82 | (1) |
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83 | (2) |
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85 | (2) |
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87 | (2) |
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87 | (2) |
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5 Communicating biotechnology to an uncertain public: the need to raise awareness |
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89 | (10) |
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89 | (1) |
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5.2 Attitudes to biotechnology |
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90 | (2) |
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5.3 The FDF communication programme on biotechnology |
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92 | (2) |
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5.4 Other industry initiatives in the UK |
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94 | (1) |
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5.5 The issue of labelling |
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94 | (1) |
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5.6 International communication initiatives |
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95 | (1) |
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96 | (3) |
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97 | (2) |
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99 | (152) |
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101 | (28) |
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6.1 Introduction and historical perspective |
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101 | (1) |
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102 | (9) |
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6.2.1 The need for a milk-clotting enzyme |
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102 | (1) |
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103 | (1) |
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6.2.3 The structure and synthesis of animal chymosin |
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104 | (1) |
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6.2.4 The genetic modification of microorganisms for chymosin production |
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104 | (3) |
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6.2.5 The industrial production of recombinant chymosins |
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107 | (1) |
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6.2.6 Technological performance of recombinant chymosins |
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107 | (1) |
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6.2.7 Health, safety and environmental issues |
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108 | (2) |
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6.2.8 Legislative and labelling position |
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110 | (1) |
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6.2.9 Consumer acceptance and market penetration |
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110 | (1) |
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6.3 Other food enzymes from GMOs |
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111 | (3) |
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6.3.1 Recombinant enzymes for high fructose corn syrup production |
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111 | (2) |
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6.3.2 Safety, legislation and labelling |
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113 | (1) |
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6.4 Protein engineering of enzymes |
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114 | (10) |
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6.4.1 The structure and function of proteins |
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115 | (2) |
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6.4.2 Site-directed mutagenesis |
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117 | (1) |
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6.4.3 Improving the properties of enzymes by protein engineering |
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118 | (6) |
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124 | (5) |
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125 | (4) |
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7 Brewing with genetically modified amylolytic yeast |
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129 | (29) |
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129 | (1) |
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7.2 The genetic modification procedure |
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130 | (6) |
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7.2.1 The genetics of amylolysis |
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130 | (1) |
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7.2.2 Yeast transformation methods |
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131 | (1) |
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7.2.3 Construction of an amylolytic strain of brewing yeast using the STA2 gene |
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132 | (2) |
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7.2.4 An alternative approach to the construction of an amylolytic strain of brewing yeast using the GA gene |
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134 | (2) |
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7.3 The technological performance of genetically modified brewing yeasts |
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136 | (8) |
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7.3.1 The yeast strain NCYC1324 [ pDVK2] |
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136 | (4) |
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7.3.2 The yeast strain NCYC1342.GA3 |
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140 | (4) |
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7.3.4 Advantages and disadvantages of genetically modified amylolytic yeasts |
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144 | (1) |
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7.4 Health, safety and environmental issues |
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144 | (4) |
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7.4.1 Containment considerations |
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144 | (1) |
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7.4.2 Transfer of `novel' genetic material to other microorganisms and survivability of the modified strains |
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145 | (1) |
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7.4.3 Pathogenicity and allergenicity |
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146 | (1) |
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7.4.4 Disposal of waste yeast - environmental considerations |
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147 | (1) |
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7.5 Legislative and labelling position |
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148 | (2) |
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7.6 Consumer acceptance and marketing |
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150 | (1) |
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151 | (4) |
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7.7.1 Improved utilization of carbohydrates: yeasts secreting β-glucanases |
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151 | (1) |
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7.7.2 Yeasts with a reduced capacity to produce diacetyl |
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152 | (1) |
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7.7.3 Yeasts with a reduced capacity to produce hydrogen sulphide |
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153 | (1) |
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7.7.4 Increased production of flavour compounds |
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154 | (1) |
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155 | (3) |
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155 | (1) |
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155 | (3) |
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158 | (16) |
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8.1 Introduction and historical perspective |
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158 | (1) |
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8.2 Traditional aproaches to yeast improvement |
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159 | (1) |
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8.3 The construction of genetically modified baker's yeast |
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160 | (3) |
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8.3.1 Transformation of yeast |
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161 | (1) |
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8.3.2 The transforming DNA |
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161 | (1) |
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8.3.3 Selection of transformants |
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162 | (1) |
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8.3.4 Self-cloning in yeast |
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163 | (1) |
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8.4 Technological properties of genetically modified baker's yeast strains |
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163 | (5) |
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8.4.1 Increased expression of MAL genes |
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163 | (2) |
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8.4.2 Yeast with an enhanced trehalose content |
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165 | (2) |
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8.4.3 Introduction of futile cycles in the glycolytic pathway |
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167 | (1) |
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168 | (6) |
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8.5.1 The impact of genome sequencing projects |
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170 | (1) |
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8.5.2 Metabolic pathway engineering |
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171 | (1) |
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172 | (2) |
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9 Starter cultures for the dairy industry |
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174 | (19) |
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174 | (2) |
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9.2 Improvement of dairy starters |
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176 | (1) |
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9.3 Genetic tools available in lactococci |
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177 | (3) |
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9.3.1 Assembling the tools |
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178 | (1) |
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9.3.2 The development of more sophisticated techniques |
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179 | (1) |
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9.4 Genetic transfer in lactic acid bacteria |
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180 | (1) |
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9.4.1 Gene exchange mechanisms |
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181 | (1) |
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9.5 Cloning and characterization of key lactococcal traits |
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181 | (2) |
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182 | (1) |
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9.5.2 Bacteriophage resistance |
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182 | (1) |
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9.6 `Natural' genetic modification of lactococcal starters |
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183 | (3) |
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9.7 Metabolic engineering of lactococci |
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186 | (2) |
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9.8 Social, cultural and legal aspects and implications |
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188 | (2) |
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190 | (3) |
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191 | (2) |
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10 Designer oils: the high oleic acid soybean |
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193 | (21) |
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10.1 Introduction and historical perspective |
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193 | (2) |
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10.2 Target applications in the genetic modification of oil |
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195 | (2) |
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10.3 Development and composition of high oleic acid soybeans |
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197 | (2) |
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10.4 Field performance of high oleic acid soybeans |
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199 | (1) |
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10.5 Technological properties of high oleic acid soybeans |
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200 | (5) |
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10.6 Health, safety and legislative issues |
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205 | (3) |
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10.7 Environmental issues |
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208 | (1) |
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10.8 Labelling of the high oleic acid soybean |
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209 | (1) |
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10.9 Consumer acceptance and marketing |
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210 | (1) |
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10.10 Future prospects: true designer oils |
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210 | (4) |
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211 | (3) |
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214 | (14) |
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11.1 Introduction and historical perspective |
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214 | (1) |
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11.2 Production process and patent status |
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215 | (2) |
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215 | (1) |
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11.2.2 High solids potato |
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216 | (1) |
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11.3 Field/process performance |
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217 | (2) |
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217 | (1) |
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11.3.2 High solids potato |
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218 | (1) |
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11.4 Technological properties |
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219 | (4) |
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219 | (1) |
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11.4.2 High solids potato |
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220 | (3) |
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11.5 Health, safety and environmental issues |
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223 | (1) |
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223 | (1) |
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11.5.2 High solids potato |
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224 | (1) |
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11.6 Legislative and labeling position |
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224 | (1) |
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224 | (1) |
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11.6.2 High solids potato |
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225 | (1) |
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11.7 Consumer acceptance and marketing |
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225 | (1) |
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225 | (1) |
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11.7.2 High solids potato |
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226 | (1) |
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226 | (2) |
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227 | (1) |
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228 | (23) |
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12.1 Introduction and historical perspective |
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228 | (2) |
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12.2 The techniques of genetic modification in cereals |
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230 | (6) |
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231 | (2) |
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233 | (1) |
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234 | (1) |
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235 | (1) |
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12.3 The application of gene technology in cereals |
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236 | (7) |
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237 | (2) |
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239 | (1) |
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240 | (3) |
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243 | (1) |
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12.4 Intellectual property and patenting issues in cereal biotechnology |
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243 | (2) |
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12.5 Health, safety and environmental issues |
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245 | (1) |
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12.6 Legislative and labelling position |
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245 | (1) |
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12.7 Consumer acceptance and marketing |
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246 | (1) |
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247 | (4) |
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247 | (4) |
Glossary |
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251 | (8) |
Index |
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259 | |