Glycan interactions with a variety of glycan binding proteins (GBPs) play a significant role in cell-cell communication, immune evasion, and pathogenic processes. Understanding these interactions is critical to regulating cellular processes and has tremendous therapeutic significance. However,
methods to study glycan interactions are relatively limited due to several structural and functional properties of glycans.
This work explores specially adapted methods of study, with advances such as glycan microarrays, glycosylated conductive polymers, Luminex bead-based arrays, metabolic labelling, cell surface engineering, and chemo enzymatic labeling. This volume serves as a valuable tool for researchers and
industry workers interested in developing and adapting technologies in the detection of glycan-GBP interactions.
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1 Recent Advancements in Arrayed Technologies and Emerging Themes in the Identification of Glycan-Protein Interactions |
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1 | (22) |
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2 Insights into Antibody-Carbohydrate Recognition from Neoglycoprotein Microarrays |
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23 | (16) |
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3 Generation of a Heparan Sulfate Mutant Cell Library and Its Application to Determine the Structure-Function Relationship of Heparan Sulfate in Facilitating FGF2-FGFR1 Signaling |
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39 | (8) |
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4 Polyvalent Glycan-Quantum Dots as Multifunctional Structural Probes for Multivalent Lectin-Carbohydrate Interactions |
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47 | (20) |
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5 Automated Identification of Lectin Fine Specificities from Glycan-Array Data |
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67 | (16) |
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6 Label-Free Biosensors for Studying Carbohydrate-Protein Interaction |
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83 | (36) |
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7 Sketching the Glycan Hallmark of Intact Cells Using Lectin Microarray |
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119 | (8) |
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8 Methods to Investigate Innate Immune Receptors and Their Carbohydrate-Based Ligands |
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127 | (22) |
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9 Morphogenesis of the Mammalian Tectorial Membrane: Unveiling the Surface Roles of a Matrix Organizer, Alpha Tectorin |
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149 | (20) |
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10 Mass Spectrometric Analysis of Protein Glycosylation |
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169 | (36) |
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| Editors' Biographies |
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205 | (4) |
| Author Index |
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209 | (2) |
| Subject Index |
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211 | |
Peng George Wang obtained his BS degree in Chemistry from Nankai University, China in 1984 and his PhD degree in organic/bioorganic chemistry from the University of California, Berkeley (UCB) in 1990. He served as faculty at the University of Miami (1994-1997), Wayne State University (1997-2003), Ohio State University (2003-2011), and Georgia State University (2011-2019) Currently, he is an endowed professor in the School of Medicine at the Southern University of Science and Technology in China. Professor Wang's main research focus is in glycoscience on glycobiology, glycochemistry, glycoanalysis, glycomics, medicinal chemistry, and chemical biology, with an emphasis on cancer immunotherapy, small molecule drug discovery, carbohydrate-based vaccines, as well as glycopeptide and glycoprotein drugs. The Wang lab has published 439 peer-reviewed research papers/reviews with a google scholar h index factor of 54. Professor Wang has edited seven books and has six issued US patents. In 1999,Wang
received the Camille Dreyfus Teacher-Scholar Award. In 2002, Professor Wang was the Horace S. Isbell Awardee from the Division of Carbohydrate Chemistry of the American Chemical Society. In 2012, he was elected as an American Association for the Advancement of Science (AAAS) fellow.
Cheng Ma has made substantial contributions to the field of analytical chemistry. Specifically, his research has focused on mass spectrometry-based glycoprotein analysis. Ma's research addresses some of most pressing issues in understanding the biological mechanics underlying disease pathogenesis and intervention. His strong background in analytical chemistry has allowed his research to be particularly original, with applications such as new methods for analyzing N-glycosylation site using mass spectrometry. Dr. Ma, as first author, corresponding, or co-author, has published journal articles in publications such as Nature Communication, Nature Protocol, Journal of the American Chemical Society,
Chemical Science, Analytical Chemistry, ACS Chemical Biology, Scientific Report, Journal of Proteome Research, Journal of Proteomics, Analyst, and Analytica Chimica Acta.
Shukkoor Muhammed Kondengaden obtained his Bachelor's in pharmacy from Al-Shifa College of Pharmacy and an MS Pharm in medicinal chemistry from the National Institute of Pharmaceutical Education and Research (NIPER) of India in 2008 and 2010, respectively. From 2010 to 2013, he worked as a senior lecturer in Sridevi College of Pharmacy. He finished his PhD (2013-2018) at Georgia State University. He currently works as a senior scientist at Chemily Glycoscience, a glycan-focused R&D firm. His area of research interests includes synthetic organic chemistry, medicinal chemistry, and glycobiology. More recently, his work focuses on the development of novel technologies for the study of glycan-glycan binding protein interactions, particularly using the principles of conductive polymers and DNA
encoding for the rapid and high throughput methods. He has developed several approaches for the multivalent presentation of glycans using multivalent linkers, DNA scaffolds, and proteins to increase the affinity of glycans to the target proteins for detection and therapeutic purposes. He has several quality publications in journals like JACS, JOC, European Journal of Medicinal Chemistry, and has multiple patents to his name.
