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El. knyga: Heterologous Protein Production in CHO Cells: Methods and Protocols

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  • Formatas: EPUB+DRM
  • Serija: Methods in Molecular Biology 2853
  • Išleidimo metai: 26-Oct-2024
  • Leidėjas: Springer-Verlag New York Inc.
  • Kalba: eng
  • ISBN-13: 9781071641040
Kitos knygos pagal šią temą:
Heterologous Protein Production in CHO Cells: Methods and ProtocolsDidesnis paveikslėlis
  • Formatas: EPUB+DRM
  • Serija: Methods in Molecular Biology 2853
  • Išleidimo metai: 26-Oct-2024
  • Leidėjas: Springer-Verlag New York Inc.
  • Kalba: eng
  • ISBN-13: 9781071641040
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This detailed new edition explores the use of Chinese hamster ovary (CHO) cells in the production of therapeutic protein products. Beyond updates on earlier methodologies, the book also delves into the genetic manipulation of CHO cells for recombinant protein production, analysis of CHO cells using proteomic and metabolomic approaches, as well as methods for the characterization of recombinant protein products, such as glycosylation and host cell protein analysis. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 





 





Authoritative and up-to-date, Heterologous Protein Production in CHO Cells: Methods and Protocols, Second Edition is an ideal guide for researchers working to enhance and accelerate CHO productive capabilities in the coming decades.
Serum-Free and Protein-Free Media for the Cultivation of Recombinant
Chinese Hamster Ovary Cells (CHO) Cell Lines.- Large-Scale Transient
Transfection of Suspension-Adapted Chinese Hamster Ovary Cells for the
Production of the Trimeric SARS-CoV-2 Spike Protein.- Splicing by Overlap
Extension PCR for the Production of Fusion Proteins.- Codon and Signal
Peptide Optimization to Enhance Therapeutic Antibody Production from CHO
Cells.- Application of CRISPR/Cas9 Genome Editing to Improve Recombinant
Protein Production in CHO Cells.- Conditional Knockdown of Endogenous
MicroRNAs in CHO Cells Using TET-ON-SanDI Sponge Vectors.- MiRNA Chaining for
Efficient Stable Overexpression to Improve Protein Quantity and Quality in
CHO Cells.- Anti-Apoptosis Engineering for Improved Protein Production from
CHO Cells.- The Omics Revolution in CHO Biology: Roadmap to Improved CHO
Productivity.- Recent Advancements in Proteomic Sample Preparation from
Recombinant Chinese Hamster Ovary Cells.- Separation and Purification of CHO
Secretome and Extracellular Vesicles for Proteome Analysis.- Phosphopeptide
Enrichment and LC-MS/MS Analysis to Study the Phosphoproteome of Recombinant
Chinese Hamster Ovary Cells.- LC-MS/MS Analysis to Study the
Ubiquitin-Modified Proteome of Recombinant Chinese Hamster Ovary Cells.-
Metabolomics of Chinese Hamster Ovary Cells.- Analytical Tools for HCP
Detection, Identification, and Quantitation.- Quantitative High-Throughput
and High-Sensitivity Glycoprofile Analysis of Therapeutic Glycoproteins Using
HILIC-FLD and ESI-MS.
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