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High Throughput Protein Expression and Purification: Methods and Protocols 2009 ed. [Kietas viršelis]

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  • Formatas: Hardback, 322 pages, aukštis x plotis: 260x193 mm, weight: 921 g, 6 Illustrations, color; 51 Illustrations, black and white; XII, 322 p. 57 illus., 6 illus. in color., 1 Hardback
  • Serija: Methods in Molecular Biology 498
  • Išleidimo metai: 20-Oct-2008
  • Leidėjas: Humana Press Inc.
  • ISBN-10: 1588298795
  • ISBN-13: 9781588298799
Kitos knygos pagal šią temą:
  • Formatas: Hardback, 322 pages, aukštis x plotis: 260x193 mm, weight: 921 g, 6 Illustrations, color; 51 Illustrations, black and white; XII, 322 p. 57 illus., 6 illus. in color., 1 Hardback
  • Serija: Methods in Molecular Biology 498
  • Išleidimo metai: 20-Oct-2008
  • Leidėjas: Humana Press Inc.
  • ISBN-10: 1588298795
  • ISBN-13: 9781588298799
Kitos knygos pagal šią temą:
Despite exciting advances in genome sequencing, isolating a protein from its expression system in its native form still presents a complex challenge. In High Throughput Protein Expression and Purification: Methods and Protocols, leading scientists detail the most successful protocols currently in use, including various high throughput cloning schemes, protein expression analysis, and production protocols. This volume describes the use of E. coli, insect, and mammalian cells, as well as cell-free systems for the production of a wide variety of proteins, including glycoproteins and membrane proteins, in order to best represent strategies that create and exploit common features to enable simplified cloning, stable expression, and purification of proteins. Written in the highly successful Methods in Molecular Biology series format, the chapters present brief introductions to the subject, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and a Notes section for tips on troubleshooting and avoiding known pitfalls.



Cutting-edge and comprehensive, High Throughput Protein Expression and Purification: Methods and Protocols is an ideal reference for protein biochemists and all those who wish to apply these easy-to-use protocols to the many applicable fields.

Recenzijos

From the reviews:





"The book consists 20 chapters, which cover methods in protein expression and purification summarized by leading experts in this area from USA, UK, and Switzerland. Numerous pictures in the text, a references list at the end of each chapter, and a subject index help better understand the presented materials. The volume is true handbook for protein biochemists, molecular biologists, biotechnologists, and other researchers and technicians who are involved in the many applicable fields." (G. Wiederschain, Biochemistry (Moscow), Vol. 74 (8), 2009)



High Throughput Protein Expression and Purification: Methods and Protocols, edited by Sharon A. Doyle, is an interesting attempt showing what one currently has at ones disposal if one wishes to make a first step toward this aim. this book should be recommended especially to researchers who would like to organize a new laboratory focused on high throughput protein production, or to improve an existing one. It can also prove helpful to those who wish to optimize individual protein expression, isolation or purification. (Marek Figlerowicz, Acta Biochimica Polonica, Vol. 56 (4), December, 2009)

Preface v
Contributors ix
High-Throughput Protein Production (HTPP): A Review of Enabling Technologies to Expedite Protein Production
1(18)
Jim Koehn
Ian Hunt
Designing Experiments for High-Throughput Protein Expression
19(12)
Stephen P. Chambers
Susanne E. Swalley
Gateway Cloning for Protein Expression
31(24)
Dominic Esposito
Leslie A. Garvey
Chacko S. Chakiath
Flexi Vector Cloning
55(20)
Paul G. Blommel
Peter A. Martin
Kory D. Seder
Russell L. Wrobel
Brian G. Fox
The Precise Engineering of Expression Vectors Using High-Throughput In-Fusion™ PCR Cloning
75(16)
Nick S. Berrow
David Alderton
Raymond J. Owens
The Polymerase Incomplete Primer Extension (PIPE) Method Applied to High-Throughput Cloning and Site-Directed Mutagenesis
91(14)
Heath E. Klock
Scott A. Lesley
A Family of LIC Vectors for High-Throughput Cloning and Purification of Proteins
105(12)
William H. Eschenfeldt
Lucy Stols
Cynthia Sanville Millard
Andrezej Joachimiak
Mark I. Donnelly
``System 48'' High-Throughput Cloning and Protein Expression Analysis
117(12)
James M. Abdullah
Andrezej Joachimiak
Frank R. Collart
Automated 96-Well Purification of Hexahistidine-Tagged Recombinant Proteins on Magne His Ni2+-Particles
129(14)
Chiann-Tso Lin
Priscilla A. Moore
Vladimir Kery
E. coli and Insect Cell Expression, Automatied Purification and Quantitative Analysis
143(14)
Stephen P. Chambers
John R. Fulghum
Douglas A. Austen
Fan Lu
Susanne E. Swalley
Hexahistidine-Tagged Maltose-Binding Protein as a Fusion Partner for the Production of Soluble Recombinant Proteins in Escherichia Coli
157(16)
Brian P. Austin
Sreedevi Nallamsetty
David S. Waugh
PHB-Intein-Mediated Protein Purification Strategy
173(12)
Alison R. Gillies
Mahmoud Reza Banki
David W. Wood
High-Throughput Biotinylation of Proteins
185(14)
Brian K. Kay
Sang Thai
Veronica V. Volgina
High-Throughput Insect Cell Protein Expression Applications
199(30)
Mirjam Buchs
Ernie Kim
Yann Pouliquen
Michael Sachs
Sabine Geisse
Marion Mahnke
Ian Hunt
High-Throughput Protein Expression Using Cell-Free System
229(16)
Kalavathy Sitaraman
Deb K. Chatterjee
The Production of Glycoproteins by Transient Expression in Mammalian Cells
245(20)
Joanne E. Nettleship
Nahid Rahman-Huq
Raymond J. Owens
High-Throughput Expression and Detergent Screening of Integral Membrane Proteins
265(8)
Said Eshaghi
Cell-Free Expression for Nanolipoprotein Particles: Building a High-Throughput Membrane Protein Solubility Platform
273(24)
Jenny A. Cappuccio
Angela K. Hinz
Edward A. Kuhn
Julia Fletcher
Erin S. Arroyo
Paul T. Henderson
Craig D. Blanchette
Vicki L. Walsworth
Michele H. Corzett
Richard J. Law
Joseph B. Pesavento
Brent W. Segelke
Todd A. Sulchek
Brett A. Chromy
Federico Katzen
Todd Peterson
Graham Bench
Wieslaw Kudlicki
Paul D. Hoeprich Jr
Matthew A. Coleman
Expression and Purification of Soluble His6 - Tagged TEV Protease
297(12)
Joseph E. Tropea
Scott Cherry
David S. Waugh
High-Throughput Protein Concentration and Buffer Exchange: Comparison of Ultrafiltration and Ammonium Sulfate Precipitation
309(6)
Priscilla A. Moore
Vladimir Kery
Index 315