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Histones: Methods and Protocols Second Edition 2025 [Kietas viršelis]

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  • Formatas: Hardback, 292 pages, aukštis x plotis: 254x178 mm, 38 Illustrations, color; 14 Illustrations, black and white; XI, 292 p. 52 illus., 38 illus. in color., 1 Hardback
  • Serija: Methods in Molecular Biology 2919
  • Išleidimo metai: 22-Apr-2025
  • Leidėjas: Springer-Verlag New York Inc.
  • ISBN-10: 1071644858
  • ISBN-13: 9781071644850
Kitos knygos pagal šią temą:
Histones: Methods and Protocols Second Edition 2025Didesnis paveikslėlis
  • Formatas: Hardback, 292 pages, aukštis x plotis: 254x178 mm, 38 Illustrations, color; 14 Illustrations, black and white; XI, 292 p. 52 illus., 38 illus. in color., 1 Hardback
  • Serija: Methods in Molecular Biology 2919
  • Išleidimo metai: 22-Apr-2025
  • Leidėjas: Springer-Verlag New York Inc.
  • ISBN-10: 1071644858
  • ISBN-13: 9781071644850
Kitos knygos pagal šią temą:
Pastovi nuoroda: https://www.kriso.lt/db/9781071644850.html
Raktažodžiai:

This second edition provides new and updated chapters on histones for molecular biologists, biochemists and geneticists. Chapters cover protein complexes that modify chromatin either by adding or removing post-translational modifications, or by exchanging histone variants within the nucleosome. Written in the highly successful Methods in Molecular Biology series format, the chapters include brief introductions to the material, lists of necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and a Notes section which highlights tips on troubleshooting and avoiding known pitfalls.

 

Authoritative and cutting-edge, Histones: Methods and Protocols, Second Edition aims to be comprehensive guide for researchers in the field.

In vitro assembly of nucleosomes for binding/remodeling assays.-
Ruthenium-catalyzed one-pot peptide ligation.- A method for H2A.Z/H2B dimer
exchange within nucleosomes in vitro.- Automated sample preparation for the
unbiased analysis of histone post-translational modifications via mass
spectrometry.- Detection of -H2A.X for Rapid Assessment of Genotoxic
Agents-Induced Double-Stranded DNA breaks by Immunofluorescence.- High
Resolution Cryo-EM Analyses of Nucleosomes.- Correlative Electron
spectroscopic Imaging (ESI) and electron tomography of chromatin.- Psoralen
crosslinking-Chromatin endogenous cleavage assay to examine histone DNA
interactions of active and inactive rRNA genes.- Genome-wide nucleosome
mapping by H3Q85C-directed chemical cleavage in Saccharomyces cerevisiae.-
Efficient in-house coupling of sample and library preparation for ChIP-seq of
histone modifications in complex plant tissues.- Chromatin
immunoprecipitation assays of histone modifications in Daphnia magna.-
Single-cell epigenomic profiling with high-throughput droplet scChIP-seq.-
Determining the Substrate Specificity of Lysine Methyltransferases.-
Identification of chromatin readers using a peptide pull-down and mass
spectrometry integrated approach.- Efficient, Conditional Depletion of
Nuclear Proteins in Saccharomyces cerevisiae.- RNA Immunoprecipitation (RIP)
from Purified Nuclei in Cells.