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El. knyga: Ice Binding Proteins: Methods and Protocols

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  • Formatas: EPUB+DRM
  • Serija: Methods in Molecular Biology 2730
  • Išleidimo metai: 09-Nov-2023
  • Leidėjas: Springer-Verlag New York Inc.
  • Kalba: eng
  • ISBN-13: 9781071635032
Kitos knygos pagal šią temą:
Ice Binding Proteins: Methods and ProtocolsDidesnis paveikslėlis
  • Formatas: EPUB+DRM
  • Serija: Methods in Molecular Biology 2730
  • Išleidimo metai: 09-Nov-2023
  • Leidėjas: Springer-Verlag New York Inc.
  • Kalba: eng
  • ISBN-13: 9781071635032
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This volume provides methods to study ice-binding proteins (IBPs), and applications involving these proteins. Chapters are divided into three parts describing identifying, isolating, and purifying ice-binding proteins, characterize both IBPs and ice-nucleating proteins (INPs), and synthesize mimics of IBPs, as well as applications involving these proteins. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols.   





Authoritative and cutting-edge, Ice Binding Proteins: Methods and Protocols aims to provide both experimental and simulations protocols to help new researchers, break-the-ice, and enter this exciting field, while also supporting established researchers broaden the scope of theirinvestigations. 
Identifying Ice-Binding Proteins in Nature.- Ice-shell purification of
ice-active compounds.- Ice Isnt the Only Crystal in Town: Structure
Determination of Ice-Binding Proteins Via X-ray Crystallography.- Large Scale
Purification of Natural Ice Binding Proteins by the Falling Water Ice
Purification Method.- The Nanoliter Osmometer - Thermal Hysteresis
Measurement.- Evaluation of ice recrystallization inhibition of ice-binding
proteins by monitoring specific ice crystals.- Measurement of Ice Nucleation
Activity of Biological Samples.- Investigating the interaction between
ice-binding proteins and ice surfaces using microfluidicdevices and cold
stages.- Quantification of the ice nucleation activity of ice-binding
proteins using a microliter droplet freezing experiment.- Measurement of
Ice-Binding Protein Activity in Highly Alkaline Environments.- Measurement of
ice binding proteins inhibition of non-ice crystal growth.- Divergent
Mechanisms of Ice Growth Inhibition by Antifreeze Proteins.- Multiscale
Molecular Dynamics Simulations of Ice-Binding Proteins.- Synthesis of polymer
mimics of ice-binding proteins.- Generating ice-binding protein-polymer
bioconjugates.- Analysis of ice binding protein evolution.
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