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Section 1 Hemoglobinopathies, Red Cell Enzymopathies and Membranopathies |
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1.1 Laboratory Investigation of Hemoglobinopathies |
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3 | (30) |
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1.2 Laboratory Investigation of Red Cell Enzymopathies and Membranopathies |
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33 | (15) |
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1.3 Molecular Biology Protocols for Detection of Hemoglobinopathies |
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48 | (25) |
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1.4 Molecular Biology Protocols for Detection of Red Cell Enzymopathies and Red Cell Membranopathies |
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73 | (12) |
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Section 2 Hemostasis and Thrombosis |
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Shrimati Dharmapal Shetty |
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2.1 Laboratory Diagnosis of Bleeding Disorders |
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85 | (38) |
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2.2 Genetic Diagnosis of Bleeding Disorders |
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123 | (44) |
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2.3 Thrombophilia Investigations |
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167 | (36) |
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Section 3 Transfusion Medicine |
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3.1 Serological Diagnosis |
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203 | (41) |
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3.2 Genotyping in Transfusion Medicine |
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244 | (15) |
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Section 4 Transfusion Transmitted Disorders |
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4.1 Hepatitis C Virus Viral Load |
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259 | (1) |
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4.2 Hepatitis C Virus Genotyping |
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260 | (2) |
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262 | (1) |
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4.4 Human Immunodeficiency Virus Viral Load |
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262 | (1) |
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4.5 Hepatitis B Virus DNA Quantitation |
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263 | (1) |
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4.6 Anti-HBs Quantitative ELISA |
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263 | (1) |
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4.7 Hepatitis B Surface Antigen ELISA |
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264 | (1) |
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264 | (3) |
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Section 5 Autoimmune Disorders |
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5.1 Sample Acceptance, Rejection, Collection and Processing |
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267 | (1) |
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5.2 Indirect Immunofluorescence Assay |
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268 | (2) |
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5.3 Line Immunoassay: Antinuclear Antibodies (ANA) Blot |
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270 | (2) |
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5.4 Rheumatic Factor Detection and Quantification |
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272 | (1) |
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5.5 Anticyclic Citrullinated Peptide (CCP) Antibodies Test |
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273 | (1) |
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274 | (1) |
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275 | (8) |
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6.1 Cytogenetics Laboratory Setup |
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283 | (1) |
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6.2 Human Chromosome Preparation |
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284 | (3) |
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6.3 Fluorescence in Situ Hybridization Using Bacterial Artificial Chromosome, PI-derived Artificial Chromosome and P1 Clones |
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287 | (7) |
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6.4 DNA Spectral Karyotyping |
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294 | (3) |
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6.5 Diagnosis and Monitoring of Chronic Myeloid Leukemia (CML) |
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297 | (6) |
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6.6 Diagnostic Workup for Myelodysplastic Syndrome (MDS) |
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303 | (3) |
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6.7 Fanconi Anemia (FA) Workup |
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306 | (7) |
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6.8 Aplastic Anemia Investigations |
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313 | (6) |
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Section 7 Primary Immunodeficiency Disorders |
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7.1 Diagnostic Approach to Primary Immunodeficiency Disorders |
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319 | (1) |
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7.2 Recognizing Clinical Manifestations of Primary Immunodeficiency Disorders |
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320 | (2) |
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7.3 Laboratory Approach to Primary Immunodeficiency Disorders |
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322 | (3) |
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7.4 Primary Immunodeficiency Screening |
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325 | (1) |
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7.5 Evaluation of Suspected Combined T- and B-cell Immunodeficiency |
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326 | (13) |
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7.6 Evaluation of Patients with B-cell Defect |
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339 | (13) |
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7.7 Evaluation of Patients with Phagocytic Defects |
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352 | (12) |
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7.8 Evaluation of Patients with Intrinsic and Innate Immunity Defects: Mendelian Susceptibility to Mycobacterial Diseases Workup |
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364 | (11) |
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7.9 Evaluation of Patients with Immune Dysregulation |
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375 | (11) |
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7.10 Evaluation of Patients with Complement Deficiency |
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386 | (1) |
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7.11 Molecular Analysis of Primary Immunodeficiency Disorders |
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387 | (10) |
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397 | (10) |
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Appendix 2 Preparation of Common Laboratory Reagents |
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407 | (9) |
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Appendix 3 Primer Sequences |
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416 | (52) |
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Appendix 4 General Molecular Biology Protocols |
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468 | (18) |
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Appendix 5 Suggested Reading |
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486 | (7) |
Index |
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493 | |