Immuno-Oncology And Immunotherapy Part B Volume 190, in the Methods in Cell Biology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. In this update, chapters include Machine learning methods to assess metastatic burden, Development of pre-clinical models, Tumor microenvironment analysis by multiparametric flow cytometry or/and mass cytometry, Measure of therapy induced cell senescence, and much more.
1. Flow cytometry analysis of myeloid derived suppressor cells using 6
color labeling
Rocķo Flores-Campos, Daniel J. Garcķa-Domķnguez, Lourdes Hontecillas-Prieto,
Carlos Jiménez-Cortegana, Luis de la Cruz-Merino and Vķctor Sįnchez-Margalet
2. In vitro screening methods of novel immune checkpoint inhibitors related
to T cell infiltration and anti-PD-1 resistance
Zhuoying He, Xiuman Zhou, Youmei Xiao and Yanfeng Gao
3. Machine learning approach to assess brain metastatic burden in
preclinical models.
Jessica Rappaport, Quanyi Chen, Tomi McGuire, Amélie Daugherty-Lopčs and
Romina Goldszmid
4. Cytofluorometric analysis of the maturation and activation of bone
marrow-derived dendritic cells to assess immunogenic cell death
Faye Naessens, Iuliia Efimova, Mariia Saviuk and Dmitri V. Krysko
5. Functional evaluation of circulating anti-cancer antibodies with a 3D
tumor cell growth inhibition assay
Francesca Ruzzi, Maria Sofia Semprini, Laura Scalambra, Stefania Angelicola,
Chiara Cappello, Olga Maria Pittino, Patrizia Nanni, Arianna Palladini and
Pier-Luigi Lollini
6. Spatial immunophenotyping of FFPE tissues by imaging mass cytometry
Carolina Mangana and Barbara B. Maier
7. Processing human colon cancer specimens for in vitro cytotoxicity assays
Sofia Mensurado, Rafael Blanco-Domķnguez, Mariana Carreira, Leandro Barros
and Bruno Silva-Santos
8. An orthotopic metastatic xenograft model of colorectal cancer
Rafael Blanco-Domķnguez, Sofia Mensurado, Leandro Barros, Mariana Carreira
and Bruno Silva-Santos
9. A preclinical mouse model of hepatic metastasis to instruct effective
treatment modalities
Arianna Pocaterra, Antonio Citro, Chiara Gnasso, Tamara Canu, Anna Tosi,
Antonio Rosato, Antonio Esposito, Lorenzo Piemonti and Anna Mondino
10. Myeloid-derived suppressor cells: Identification and function
Paola Vacca, Maria Teresa Bilotta, Lorenzo Moretta and Nicola Tumino
11. Measuring the impact of therapy-induced senescence on NK cell phenotypes
in cancer
Shreya R. Chowdhury, Katherine C. Murphy, Chaitanya N. Parikh, Kelly D.
DeMarco, Lin Zhou and Marcus Ruscetti
12. Selective expansion of anti-tumor innate lymphocytes in long-term
cultures after a single BCG pulse
Marķa-José Felgueres, Gloria Esteso, Nacho Aguiló and Mar Valés-Gómez
Lorenzo Galluzzi is Assistant Professor of Cell Biology in Radiation Oncology at the Department of Radiation Oncology of the Weill Cornell Medical College, Honorary Assistant Professor Adjunct with the Department of Dermatology of the Yale School of Medicine, Honorary Associate Professor with the Faculty of Medicine of the University of Paris, and Faculty Member with the Graduate School of Biomedical Sciences and Biotechnology of the University of Ferrara, the Graduate School of Pharmacological Sciences of the University of Padova, and the Graduate School of Network Oncology and Precision Medicine of the University of Rome La Sapienza. Moreover, he is Associate Director of the European Academy for Tumor Immunology and Founding Member of the European Research Institute for Integrated Cellular Pathology.
Galluzzi is best known for major experimental and conceptual contributions to the fields of cell death, autophagy, tumor metabolism and tumor immunology. He has published over 450 articles in international peer-reviewed journals and is the Editor-in-Chief of four journals: OncoImmunology (which he co-founded in 2011), International Review of Cell and Molecular Biology, Methods in Cell biology, and Molecular and Cellular Oncology (which he co-founded in 2013). Additionally, he serves as Founding Editor for Microbial Cell and Cell Stress, and Associate Editor for Cell Death and Disease, Pharmacological Research and iScience. Norma received her masters degree in health biology from Paris Saclay. In 2013, she joined the laboratory of Dr. Guido Kroemer at the Centre de Recherche des Cordeliers (Paris, France) and at Gustave Roussy (Villejuif, France), the largest center for oncological patients in Europe. She worked on several projects linked to immunosurveillance, culminating with her obtaining her PhD in 2017 with an original work on Immunogenic stress and death of cancer cells: Contribution of antigenicity vs adjuvanticity to immunosurveillance. She then moved to Weill Cornell Medicine to join the program in radiation and immunity under the mentorship of Dr. Galluzzi. Her current research is focused on investigating resistance to immunotherapy in a mouse model of HR+ breast cancer.
Maud Charpentier received her M.Sc. in Cellular Biology and her Ph.D. in Immunology from the University of Nantes, France. She chose to pursue an academic career and continued her postdoctoral training in the United States. She joined the Department of Radiation Oncology at Weill Cornell Medicine under the mentorship of Dr. Sandra Demaria. Maud has a long-standing interest in the anti-tumor immune response and its role in controlling cancer progression and treatment outcomes. Her research focuses on understanding the synergy between radiation therapy and immunotherapy in solid tumors, with the aim of overcoming resistance to treatment and developing innovative therapeutic approaches in preclinical models.
