This practical, hands-on video training programme is designed for pathologists and laboratory scientists who are beginning to use "in situ" polymerase chain reaction (PCR) - a diagnostic technique for infectious diseases and cancer. This video presents a step-by-step demonstration that shows viewers how to succesfully use the technique in their own laboratories. Part 1 of the video reviews key preparatory steps and includes information on the protease, fixative, hot start manoeuvre and reagents. Part 2 demostrates the actual techniques of "in situ" PCR and "in situ" hybridization. The third part of the video goes on to explain the basics of molecular biology and histology so that viewers can interpret "in situ" PCR findings.
