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Long Non-Coding RNAs: Methods and Protocols Second Edition 2021 [Kietas viršelis]

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  • Formatas: Hardback, 308 pages, aukštis x plotis: 254x178 mm, weight: 809 g, 41 Illustrations, color; 7 Illustrations, black and white; XIII, 308 p. 48 illus., 41 illus. in color., 1 Hardback
  • Serija: Methods in Molecular Biology 2372
  • Išleidimo metai: 21-Aug-2021
  • Leidėjas: Springer-Verlag New York Inc.
  • ISBN-10: 107161696X
  • ISBN-13: 9781071616963
Kitos knygos pagal šią temą:
Long Non-Coding RNAs: Methods and Protocols Second Edition 2021Didesnis paveikslėlis
  • Formatas: Hardback, 308 pages, aukštis x plotis: 254x178 mm, weight: 809 g, 41 Illustrations, color; 7 Illustrations, black and white; XIII, 308 p. 48 illus., 41 illus. in color., 1 Hardback
  • Serija: Methods in Molecular Biology 2372
  • Išleidimo metai: 21-Aug-2021
  • Leidėjas: Springer-Verlag New York Inc.
  • ISBN-10: 107161696X
  • ISBN-13: 9781071616963
Kitos knygos pagal šią temą:
Pastovi nuoroda: https://www.kriso.lt/db/9781071616963.html
Raktažodžiai:

This second edition provides a broad spectrum of methods used in long non-coding RNAs (lncRNA) research, ranging from computational annotation of lncRNA genes to molecular and cellular analyses of the function of individual lncRNA. Chapters guide readers through studies used to circular RNAs, RNA splicing, and findings on lncRNA in human diseases. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 

Authoritative and cutting-edge, Long Non-Coding RNAs: Methods and Protocols,Second Edition aims to ensure successful results in the further study of this vital field.


LncRNA pulldown Combined with Mass Spectrometry to Identify the Novel
lncRNA-associated Proteins.- Cross-Linking Immunoprecipitation and qPCR
(CLIP-qPCR) Analysis to Map Interactions of Long Noncoding RNAs with
Canonical and Non-canonical RNA-binding Proteins.- Characterization of Long
Non-coding RNA Associated Proteins by RNA-immunoprecipitation.- Isolation of
Protein Complexes Associated with Long Non-Coding RNAs.- Detection of Long
Noncoding RNA Expression by Real-time PCR.- Profiling Long Non-coding RNA
expression using Custom-Designed Microarray.- Long Non-coding RNA Expression
Profiling using Arraystar LncRNA Microarrays.- Nuclear RNA Isolation and
Sequencing.- Capturing Endogenous Long Noncoding RNAs and Their Binding
Proteins Using Chromatin Isolation by RNA Purification.- Purification and
Structural Characterization of the Long Noncoding RNAs.- Simultaneous RNA-DNA
FISH.- Highly Resolved Detection of Long Non-coding RNAsIn Situ.- Detection
of Long Non-coding RNA Expression by Non-radioactive Northern
Blots.- Assessment of in vivo siRNA Delivery in Cancer Mouse Models.- Genetic
Editing of Long Non-Coding RNA Using CRISPR/Cas9
Technology.- Characterization of Circular RNAs.- Identification of
circRNA-Interacting Proteins by Affinity Pulldown.- Exploration to Circular
RNA Interactomes by RNA Pull-Down Method.- Methods for Characterization of
Alternative RNA Splicing.- Computational analysis of lncRNA from cDNA
sequences.- Characterizing miRNA-lncRNA interplay.- Illuminating lncRNA
Function through Target Prediction.- Long Noncoding RNAs: An Overview.