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Official Methods of Analysis of AOAC INTERNATIONAL |
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List of Changes for the 22nd Edition, 2023 |
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xi | |
Important Notices |
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xvii | |
Preface |
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xix | |
About the Association |
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xx | |
Guide to Method Format |
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xxiii | |
Definition of Terms and Explanatory Notes |
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xxiv | |
AOAC Official Methods™ Program |
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xxxi | |
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Standard Method Performance Requirements (SMPRs®) |
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1 | (1) |
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Stakeholder Panel on Marine and Freshwater Foods |
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2 | (1) |
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Stakeholder Panel on Agent Detection Assays |
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3 | (44) |
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Stakeholder Panel on Endocrine Disruptors |
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47 | (1) |
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Stakeholder Panel on Infant Formula and Adult Nutritionals |
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48 | (53) |
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Food-Analytical (Assorted) |
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101 | (46) |
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Stakeholder Panel on Dietary Supplements |
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147 | (79) |
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Food Microbiology/Gluten and Food Allergens |
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226 | (15) |
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AOAC Cannabis Analytical Science Program |
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241 | (35) |
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AOAC Food Authenticity Methods Program |
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276 | (29) |
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Glycerol Esters of Wood Rosin |
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305 | (6) |
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311 | |
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1 Agricultural Liming Materials |
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1 | (1) |
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1.1 Liming Materials---General |
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1 | (2) |
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1.2 Calcium Silicate Slags |
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3 | (1) |
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1.3 Gravimetric Elemental Analyses |
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4 | (1) |
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1.4 Chelometric Elemental Analyses |
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5 | (1) |
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1.5 Colorimetric Elemental Analyses |
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6 | |
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1 | (1) |
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1 | (3) |
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4 | (1) |
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5 | (6) |
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11 | (10) |
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21 | (7) |
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28 | (40) |
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68 | (4) |
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72 | |
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1 | (1) |
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1 | (1) |
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1 | (5) |
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6 | (10) |
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16 | (10) |
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26 | (5) |
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31 | (2) |
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33 | |
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1 | (1) |
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1 | (22) |
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23 | (12) |
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35 | (1) |
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36 | (3) |
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39 | (4) |
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43 | (11) |
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54 | (12) |
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66 | (10) |
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76 | (2) |
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78 | |
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1 | (1) |
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5.1 Feeds---General Methods |
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1 | (42) |
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5.2 Chemical Methods for Antibiotics |
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43 | (15) |
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5.3 Microbiological Methods for Antibiotics |
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58 | |
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Common and Chemical Names of Drugs |
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84 | |
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1 | (1) |
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6.1 Phenol Coefficient Methods |
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1 | (2) |
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6.2 Hard Surface Carrier Test Methods |
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3 | (16) |
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19 | |
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1 | (1) |
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1 | (7) |
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7.2 Inorganic and Organometallic Pesticides and Adjuvants |
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8 | (16) |
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24 | (20) |
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44 | (10) |
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7.5 Pesticides Related to Natural Products and Their Synergists |
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54 | (13) |
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7.6 Organohalogen Pesticides |
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67 | (35) |
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7.7 Thiophosphorus and Other Organophosphorus Pesticides |
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102 | (25) |
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7.8 Miscellaneous Pesticides |
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127 | |
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Common and Chemical Names of Pesticides |
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136 | |
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1 | (1) |
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9 Metals and Other Elements at Trace Levels in Foods |
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1 | (1) |
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1 | (26) |
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9.2 Single-Element Methods |
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27 | |
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10 Pesticide and Industrial Chemical Residues |
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1 | (1) |
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10.1 General Multiresidue Methods |
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1 | (25) |
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10.2 Organochlorine Residues |
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26 | (9) |
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10.3 Organophosphorus Residues |
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35 | (14) |
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49 | (1) |
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49 | (7) |
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56 | (39) |
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95 | (34) |
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129 | |
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Common and Chemical Names of Pesticides |
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132 | |
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1 | (1) |
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1 | (30) |
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31 | |
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1 | (1) |
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1 | (1) |
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14 Veterinary Analytical Toxicology |
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1 | (1) |
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1 | (1) |
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1 | (5) |
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15.2 Deodorants and Antiperspirants |
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6 | (5) |
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11 | (1) |
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11 | (2) |
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13 | (1) |
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14 | |
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16 Extraneous Materials: Isolation |
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1 | (1) |
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1 | (10) |
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16.2 Beverages and Beverage Materials |
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11 | (2) |
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13 | (4) |
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16.4 Nuts and Nut Products |
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17 | (2) |
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16.5 Grains and Their Products |
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19 | (6) |
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25 | (2) |
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27 | (1) |
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16.8 Eggs and Egg Products |
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28 | (1) |
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16.9 Poultry, Meat, and Fish and Other Marine Products |
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29 | (3) |
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16.10 Fruits and Fruit Products |
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32 | (2) |
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16.11 Snack Food Products |
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34 | (1) |
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16.12 Sugars and Sugar Products |
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35 | (1) |
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16.13 Vegetables and Vegetable Products |
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36 | (6) |
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16.14 Spices and Other Condiments |
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42 | (10) |
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52 | (7) |
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59 | (10) |
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69 | (3) |
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16.18 Fruits and Fruit Products |
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72 | (1) |
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16.19 Vegetables and Vegetable Products |
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73 | |
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17 Microbiological Methods |
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1 | (1) |
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17.1 Eggs and Egg Products |
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1 | (2) |
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17.2 Chilled, Frozen, Precooked, or Prepared Foods, and Nut Meats |
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3 | (30) |
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33 | (24) |
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57 | (64) |
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121 | (17) |
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17.6 Sterility (Commercial) of Foods (Canned, Low Acid) |
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138 | (5) |
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143 | (9) |
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152 | (4) |
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156 | (138) |
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294 | (76) |
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370 | (4) |
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374 | (2) |
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376 | (1) |
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377 | (10) |
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17.15 General Screening Methods |
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387 | (4) |
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391 | |
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1 | (1) |
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1 | (1) |
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2 | (1) |
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3 | (2) |
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5 | (13) |
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18 | (3) |
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21 | (5) |
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26 | (2) |
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28 | (4) |
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32 | (12) |
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18.10 Microchemical Tests |
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44 | (8) |
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52 | (1) |
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53 | (1) |
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54 | (1) |
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55 | (1) |
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56 | |
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Common and Chemical Names of Drugs |
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57 | |
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1 | (1) |
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1 | (1) |
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2 | (5) |
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19.3 Analgesics and Antipyretics |
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7 | (5) |
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19.4 Hypnotics and Sedatives |
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12 | (8) |
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20 | (3) |
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23 | (3) |
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26 | (4) |
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19.8 Other Sulfur-Containing Drugs |
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30 | |
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Common and Chemical Names of Drugs |
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35 | |
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1 | (1) |
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1 | (5) |
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6 | (1) |
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7 | (1) |
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8 | (1) |
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9 | (2) |
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11 | (3) |
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20.7 Physostigmine Alkaloids |
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14 | (2) |
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16 | (1) |
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17 | (8) |
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25 | (1) |
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26 | (3) |
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20.12 Other Natural Products |
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29 | |
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Common and Chemical Names of Drugs |
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34 | |
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1 | (1) |
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1 | (2) |
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3 | (3) |
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21.3 Progestational Steroids |
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6 | (2) |
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21.4 Adrenocortico Steroids |
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8 | (6) |
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14 | |
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Common and Chemical Names of Drugs |
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15 | |
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1 | (1) |
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Common and Chemical Names of Drugs |
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14 | |
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23 Drugs and Feed Additives in Animal Tissues |
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1 | (1) |
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Common and Chemical Names of Drugs |
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78 | |
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1 | (1) |
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25 Baking Powders and Baking Chemicals |
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1 | (1) |
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1 | (1) |
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1 | (17) |
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26.2 Cordials and Liqueurs |
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18 | |
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27 Malt Beverages and Brewing Materials |
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1 | (1) |
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1 | (21) |
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22 | (1) |
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23 | (7) |
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30 | (2) |
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32 | (3) |
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27.6 Brewing Sugars and Syrups |
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35 | (2) |
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37 | (1) |
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38 | (1) |
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39 | |
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1 | (1) |
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1 | (18) |
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19 | (1) |
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20 | |
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29 Nonalcoholic Beverages and Concentrates |
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1 | (1) |
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1 | (1) |
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31 Cacao Bean and Its Products |
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1 | (1) |
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1 | (2) |
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3 | (5) |
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8 | (1) |
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8 | (2) |
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10 | |
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1 | (1) |
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1 | (45) |
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32.2 Wheat, Rye, Oats, Corn, Buckwheat, Rice, Barley, and Soybeans and Their Products Except Cereal Adjuncts |
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46 | (36) |
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82 | (4) |
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86 | (2) |
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32.5 Macaroni, Egg Noodles, and Similar Products |
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88 | |
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1 | (1) |
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1 | (3) |
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4 | (66) |
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70 | (6) |
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33.4 Evaporated and Condensed Milk |
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76 | (1) |
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33.5 Dried Milk, Nonfat Dry Milk, and Malted Milk |
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77 | (4) |
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81 | (5) |
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86 | (14) |
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33.8 Ice Cream and Frozen Desserts |
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100 | |
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1 | (1) |
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35 Fish and Other Marine Products |
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1 | (1) |
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1 | (1) |
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1 | (1) |
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36.2 Vanilla Extract and Its Substitutes |
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1 | (14) |
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36.3 Lemon, Orange, and Lime Extracts, Flavors, and Oils |
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15 | (7) |
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22 | (1) |
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36.5 Cassia, Cinnamon, and Clove Extracts |
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23 | (1) |
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36.6 Flavor Extracts and Toilet Preparations |
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24 | |
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37 Fruits and Fruit Products |
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1 | (1) |
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38 Gelatin, Dessert Preparations, and Mixes |
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1 | (1) |
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39 Meat and Meat Products |
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1 | (1) |
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1 | (1) |
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1 | (1) |
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42 Vegetable Products, Processed |
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1 | (1) |
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1 | (8) |
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9 | (1) |
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10 | |
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43 Spices and Other Condiments |
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1 | (1) |
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44 Sugars and Sugar Products |
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1 | (1) |
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1 | (20) |
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44.2 Molasses and Molasses Products |
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21 | (5) |
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26 | (2) |
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28 | (12) |
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44.5 Maple, Sap, Maple Syrup, Maple Syrup Products |
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40 | (10) |
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50 | (1) |
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44.7 Corn Syrups and Other Starch-Derived Sweeteners |
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50 | |
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45 Vitamins and Other Nutrients |
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1 | (1) |
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1 | (52) |
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45.2 Microbiological Methods |
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53 | (17) |
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70 | (11) |
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45.4 Nutritionally Related Components |
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81 | |
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1 | (1) |
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46.1 Separation and Identification of Color Additives in Foods, Drugs, and Cosmetics |
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1 | (12) |
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13 | (7) |
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46.3 Subsidiary and Lower Sulfonated Dyes |
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20 | (1) |
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46.4 Metals and Other Elements |
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21 | (3) |
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24 | (1) |
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25 | |
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47 Food Additives: Direct |
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1 | (1) |
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1 | (1) |
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1 | (15) |
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47.3 Chemical Preservatives |
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16 | (30) |
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46 | (1) |
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47 | (2) |
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49 | |
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48 Food Additives: Indirect |
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1 | (1) |
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1 | (1) |
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1 | (1) |
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2 | (43) |
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45 | (10) |
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55 | (2) |
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57 | (6) |
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63 | (12) |
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75 | (6) |
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81 | (1) |
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82 | (4) |
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86 | (36) |
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122 | |
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50 Infant Formulas, Baby Foods, and Enteral Products |
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1 | (1) |
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1 | (83) |
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84 | (42) |
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126 | (8) |
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134 | (4) |
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138 | (11) |
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50.6 Choline and Carnitine |
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149 | (14) |
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163 | (3) |
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166 | (8) |
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50.9 Sodium Monofluoroacetate |
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174 | (15) |
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50.10 Minerals and Trace Elements |
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189 | (4) |
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193 | (9) |
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202 | (6) |
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208 | (14) |
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222 | (12) |
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234 | (11) |
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50.16 3-Monochloropropane-1,2-diol (MCPD) Esters and Glycidyl Esters (GE) |
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245 | (13) |
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50.17 Galactooligosaccharides (GOS) |
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258 | |
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1 | (1) |
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1 | (9) |
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10 | (3) |
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13 | (5) |
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18 | (5) |
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23 | (3) |
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26 | (3) |
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29 | (2) |
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31 | (2) |
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33 | (4) |
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37 | (5) |
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51.11 Phosphodiesterase Type 5 Inhibitors |
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42 | (4) |
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46 | (1) |
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47 | (4) |
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51 | (3) |
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54 | (3) |
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57 | (9) |
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66 | (1) |
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67 | (2) |
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69 | (2) |
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71 | (3) |
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74 | (6) |
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80 | |
Appendix A Standard Solutions and Reference Materials |
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1 | (1) |
Appendix B Laboratory Safety |
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1 | (1) |
Appendix C Reference Tables |
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1 | (1) |
Appendix D Guidelines for Collaborative Study Procedures to Validate Characteristics of a Method of Analysis |
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1 | (1) |
Appendix E Laboratory Quality Assurance |
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1 | (1) |
Appendix F Guidelines for Standard Method Performance Requirements |
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1 | (1) |
Appendix G Procedures and Guidelines for the Use of AOAC Voluntary Consensus Standards to Evaluate Characteristics of a Method of Analysis |
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1 | (1) |
Appendix H Probability of Detection (POD) as a Statistical Model for the Validation of Qualitative Methods |
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1 | (1) |
Appendix I Aoac International Methods Committee Guidelines for Validation of Biological Threat Agent Methods and/or Procedures |
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1 | (1) |
Appendix J Aoac International Methods Committee Guidelines for Validation of Microbiological Methods for Food and Environmental Surfaces |
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1 | (1) |
Appendix K Guidelines for Dietary Supplements and Botanicals |
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1 | (1) |
Appendix L AOAC Recommended Guidelines for Stakeholder Panel on Infant Formula and Adult Nutritionals (SPIFAN) Single-Laboratory Validation |
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1 | (1) |
Appendix M Validation Procedures for Quantitative Food Allergen ELISA Methods: Community Guidance and Best Practices |
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1 | (1) |
Appendix N ISPAM Guidelines for Validation of Qualitative Binary Chemistry Methods |
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1 | (1) |
Appendix O Environmental Factors for Validating Biological Threat Agent Detection Assays |
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1 | (1) |
Appendix P Guidance for Soil Collection, Characterization, and Application for Biothreat Agent Detection / Methods and Site Evaluations |
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1 | (1) |
Appendix Q Recommendations for Developing Molecular Assays for Microbial Pathogen Detection Using Modern In Silico Approaches |
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1 | (1) |
Appendix R Guidelines for Verifying and Documenting the Relationships Between Microbial Cultures |
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1 | (1) |
Subject Index |
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1 | (1) |
SMPR Index |
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1 | (1) |
Index of Method Numbers |
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1 | (1) |
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Official Methods of Analysis of AOAC INTERNATIONAL |
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|
|
|
|
|
|
List of Changes for the 22nd Edition, 2023 |
|
|
xi | |
Important Notices |
|
xvii | |
Preface |
|
xix | |
About the Association |
|
xx | |
Guide to Method Format |
|
xxiii | |
Definition of Terms and Explanatory Notes |
|
xxiv | |
AOAC® Official Methods™ Program |
|
xxxi | |
|
Standard Method Performance Requirements (SMPRs9) |
|
|
1 | (1) |
|
|
1 | (1) |
|
Stakeholder Panel on Marine and Freshwater Foods |
|
|
2 | (1) |
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Stakeholder Panel on Agent Detection Assays |
|
|
3 | (44) |
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Stakeholder Panel on Endocrine Disruptors |
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47 | (1) |
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Stakeholder Panel on Infant Formula and Adult Nutritionals |
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48 | (53) |
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Food-Analytical (Assorted) |
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101 | (46) |
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Stakeholder Panel on Dietary Supplements |
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147 | (79) |
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Food Microbiology/Gluten and Food Allergens |
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226 | (15) |
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AOAC Cannabis Analytical Science Program |
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241 | (35) |
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AOAC Food Authenticity Methods Program |
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276 | (29) |
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Glycerol Esters of Wood Rosin |
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|
305 | (6) |
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|
311 | |
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1 Agricultural Liming Materials |
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1 | (1) |
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1.1 Liming Materials---General |
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1 | (2) |
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1.2 Calcium Silicate Slags |
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3 | (1) |
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1.3 Gravimetric Elemental Analyses |
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4 | (1) |
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1.4 Chelometric Elemental Analyses |
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5 | (1) |
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1.5 Colorimetric Elemental Analyses |
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6 | |
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1 | (1) |
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2.1 Fertilizers---General |
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1 | (3) |
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4 | (1) |
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5 | (6) |
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11 | (10) |
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21 | (7) |
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28 | (40) |
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68 | (4) |
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72 | |
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1 | (1) |
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1 | (1) |
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1 | (5) |
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6 | (10) |
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16 | (10) |
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26 | (5) |
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31 | (2) |
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33 | |
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1 | (1) |
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4.1 Animal Feed---General |
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1 | (22) |
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23 | (12) |
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35 | (1) |
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36 | (3) |
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39 | (4) |
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43 | (11) |
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54 | (12) |
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66 | (10) |
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76 | (2) |
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|
78 | |
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1 | (1) |
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5.1 Feeds---General Methods |
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1 | (42) |
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5.2 Chemical Methods for Antibiotics |
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|
43 | (15) |
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5.3 Microbiological Methods for Antibiotics |
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|
58 | |
|
Common and Chemical Names of Drugs |
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|
84 | |
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|
1 | (1) |
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6.1 Phenol Coefficient Methods |
|
|
1 | (2) |
|
6.2 Hard Surface Carrier Test Methods |
|
|
3 | (16) |
|
|
19 | |
|
|
1 | (1) |
|
|
1 | (7) |
|
7.2 Inorganic and Organometallic Pesticides and Adjuvants |
|
|
8 | (16) |
|
|
24 | (20) |
|
|
44 | (10) |
|
7.5 Pesticides Related to Natural Products and Their Synergists |
|
|
54 | (13) |
|
7.6 Organohalogen Pesticides |
|
|
67 | (35) |
|
7.7 Thiophosphorus and Other Organophosphorus Pesticides |
|
|
102 | (25) |
|
7.8 Miscellaneous Pesticides |
|
|
127 | |
|
Common and Chemical Names of Pesticides |
|
|
136 | |
|
|
1 | (1) |
|
9 Metals and Other Elements at Trace Levels in Foods |
|
|
1 | (1) |
|
|
1 | (26) |
|
9.2 Single-Element Methods |
|
|
27 | |
|
10 Pesticide and Industrial Chemical Residues |
|
|
1 | (1) |
|
10.1 General Multiresidue Methods |
|
|
1 | (25) |
|
10.2 Organochlorine Residues |
|
|
26 | (9) |
|
10.3 Organophosphorus Residues |
|
|
35 | (14) |
|
|
49 | (1) |
|
|
49 | (7) |
|
|
56 | (39) |
|
|
95 | (34) |
|
|
129 | |
|
Common and Chemical Names of Pesticides |
|
|
132 | |
|
|
1 | (1) |
|
|
1 | (30) |
|
|
31 | |
|
|
1 | (1) |
|
|
1 | (1) |
|
14 Veterinary Analytical Toxicology |
|
|
1 | (1) |
|
|
1 | (1) |
|
|
1 | (5) |
|
15.2 Deodorants and Antiperspirants |
|
|
6 | (5) |
|
|
11 | (1) |
|
|
11 | (2) |
|
|
13 | (1) |
|
|
14 | |
|
16 Extraneous Materials: Isolation |
|
|
1 | (1) |
|
|
1 | (10) |
|
16.2 Beverages and Beverage Materials |
|
|
11 | (2) |
|
|
13 | (4) |
|
16.4 Nuts and Nut Products |
|
|
17 | (2) |
|
16.5 Grains and Their Products |
|
|
19 | (6) |
|
|
25 | (2) |
|
|
27 | (1) |
|
16.8 Eggs and Egg Products |
|
|
28 | (1) |
|
16.9 Poultry, Meat, and Fish and Other Marine Products |
|
|
29 | (3) |
|
16.10 Fruits and Fruit Products |
|
|
32 | (2) |
|
16.11 Snack Food Products |
|
|
34 | (1) |
|
16.12 Sugars and Sugar Products |
|
|
35 | (1) |
|
16.13 Vegetables and Vegetable Products |
|
|
36 | (6) |
|
16.14 Spices and Other Condiments |
|
|
42 | (10) |
|
|
52 | (7) |
|
|
59 | (10) |
|
|
69 | (3) |
|
16.18 Fruits and Fruit Products |
|
|
72 | (1) |
|
16.19 Vegetables and Vegetable Products |
|
|
73 | |
|
17 Microbiological Methods |
|
|
1 | (1) |
|
17.1 Eggs and Egg Products |
|
|
1 | (2) |
|
17.2 Chilled, Frozen, Precooked, or Prepared Foods, and Nut Meats |
|
|
3 | (30) |
|
|
33 | (24) |
|
|
57 | (64) |
|
|
121 | (17) |
|
17.6 Sterility (Commercial) of Foods (Canned, Low Acid) |
|
|
138 | (5) |
|
|
143 | (9) |
|
|
152 | (4) |
|
|
156 | (138) |
|
|
294 | (76) |
|
|
370 | (4) |
|
|
374 | (2) |
|
|
376 | (1) |
|
|
377 | (10) |
|
17.15 General Screening Methods |
|
|
387 | (4) |
|
|
391 | |
|
|
1 | (1) |
|
|
1 | (1) |
|
|
2 | (1) |
|
|
3 | (2) |
|
|
5 | (13) |
|
|
18 | (3) |
|
|
21 | (5) |
|
|
26 | (2) |
|
|
28 | (4) |
|
|
32 | (12) |
|
18.10 Microchemical Tests |
|
|
44 | (8) |
|
|
52 | (1) |
|
|
53 | (1) |
|
|
54 | (1) |
|
|
55 | (1) |
|
|
56 | |
|
Common and Chemical Names of Drugs |
|
|
57 | |
|
|
1 | (1) |
|
|
1 | (1) |
|
|
2 | (5) |
|
19.3 Analgesics and Antipyretics |
|
|
7 | (5) |
|
19.4 Hypnotics and Sedatives |
|
|
12 | (8) |
|
|
20 | (3) |
|
|
23 | (3) |
|
|
26 | (4) |
|
19.8 Other Sulfur-Containing Drugs |
|
|
30 | |
|
Common and Chemical Names of Drugs |
|
|
35 | |
|
|
1 | (1) |
|
|
1 | (5) |
|
|
6 | (1) |
|
|
7 | (1) |
|
|
8 | (1) |
|
|
9 | (2) |
|
|
11 | (3) |
|
20.7 Physostigmine Alkaloids |
|
|
14 | (2) |
|
|
16 | (1) |
|
|
17 | (8) |
|
|
25 | (1) |
|
|
26 | (3) |
|
20.12 Other Natural Products |
|
|
29 | |
|
Common and Chemical Names of Drugs |
|
|
34 | |
|
|
1 | (1) |
|
|
1 | (2) |
|
|
3 | (3) |
|
21.3 Progestational Steroids |
|
|
6 | (2) |
|
21.4 Adrenocortico Steroids |
|
|
8 | (6) |
|
|
14 | |
|
Common and Chemical Names of Drugs |
|
|
15 | |
|
|
1 | (1) |
|
Common and Chemical Names of Drugs |
|
|
14 | |
|
23 Drugs and Feed Additives in Animal Tissues |
|
|
1 | (1) |
|
Common and Chemical Names of Drugs |
|
|
78 | |
|
|
1 | (1) |
|
25 Baking Powders and Baking Chemicals |
|
|
1 | (1) |
|
|
1 | (1) |
|
|
1 | (17) |
|
26.2 Cordials and Liqueurs |
|
|
18 | |
|
27 Malt Beverages and Brewing Materials |
|
|
1 | (1) |
|
|
1 | (21) |
|
|
22 | (1) |
|
|
23 | (7) |
|
|
30 | (2) |
|
|
32 | (3) |
|
27.6 Brewing Sugars and Syrups |
|
|
35 | (2) |
|
|
37 | (1) |
|
|
38 | (1) |
|
|
39 | |
|
|
1 | (1) |
|
|
1 | (18) |
|
|
19 | (1) |
|
|
20 | |
|
29 Nonalcoholic Beverages and Concentrates |
|
|
1 | (1) |
|
|
1 | (1) |
|
31 Cacao Bean and Its Products |
|
|
1 | (1) |
|
|
1 | (2) |
|
|
3 | (5) |
|
|
8 | (1) |
|
|
8 | (2) |
|
|
10 | |
|
|
1 | (1) |
|
|
1 | (45) |
|
32.2 Wheat, Rye, Oats, Corn, Buckwheat, Rice, Barley, and Soybeans and Their Products Except Cereal Adjuncts |
|
|
46 | (36) |
|
|
82 | (4) |
|
|
86 | (2) |
|
32.5 Macaroni, Egg Noodles, and Similar Products |
|
|
88 | |
|
|
1 | (1) |
|
|
1 | (3) |
|
|
4 | (66) |
|
|
70 | (6) |
|
33.4 Evaporated and Condensed Milk |
|
|
76 | (1) |
|
33.5 Dried Milk, Nonfat Dry Milk, and Malted Milk |
|
|
77 | (4) |
|
|
81 | (5) |
|
|
86 | (14) |
|
33.8 Ice Cream and Frozen Desserts |
|
|
100 | |
|
|
1 | (1) |
|
35 Fish and Other Marine Products |
|
|
1 | (1) |
|
|
1 | (1) |
|
|
1 | (1) |
|
36.2 Vanilla Extract and Its Substitutes |
|
|
1 | (14) |
|
36.3 Lemon, Orange, and Lime Extracts, Flavors, and Oils |
|
|
15 | (7) |
|
|
22 | (1) |
|
36.5 Cassia, Cinnamon, and Clove Extracts |
|
|
23 | (1) |
|
36.6 Flavor Extracts and Toilet Preparations |
|
|
24 | |
|
37 Fruits and Fruit Products |
|
|
1 | (1) |
|
38 Gelatin, Dessert Preparations, and Mixes |
|
|
1 | (1) |
|
39 Meat and Meat Products |
|
|
1 | (1) |
|
|
1 | (1) |
|
|
1 | (1) |
|
42 Vegetable Products, Processed |
|
|
1 | (1) |
|
|
1 | (8) |
|
|
9 | (1) |
|
|
10 | |
|
43 Spices and Other Condiments |
|
|
1 | (1) |
|
44 Sugars and Sugar Products |
|
|
1 | (1) |
|
|
1 | (20) |
|
44.2 Molasses and Molasses Products |
|
|
21 | (5) |
|
|
26 | (2) |
|
|
28 | (12) |
|
44.5 Maple, Sap, Maple Syrup, Maple Syrup Products |
|
|
40 | (10) |
|
|
50 | (1) |
|
44.7 Corn Syrups and Other Starch-Derived Sweeteners |
|
|
50 | |
|
45 Vitamins and Other Nutrients |
|
|
1 | (1) |
|
|
1 | (52) |
|
45.2 Microbiological Methods |
|
|
53 | (17) |
|
|
70 | (11) |
|
45.4 Nutritionally Related Components |
|
|
81 | |
|
|
1 | (1) |
|
46.1 Separation and Identification of Color Additives in Foods, Drugs, and Cosmetics |
|
|
1 | (12) |
|
|
13 | (7) |
|
46.3 Subsidiary and Lower Sulfonated Dyes |
|
|
20 | (1) |
|
46.4 Metals and Other Elements |
|
|
21 | (3) |
|
|
24 | (1) |
|
|
25 | |
|
47 Food Additives: Direct |
|
|
1 | (1) |
|
|
1 | (1) |
|
|
1 | (15) |
|
47.3 Chemical Preservatives |
|
|
16 | (30) |
|
|
46 | (1) |
|
|
47 | (2) |
|
|
49 | |
|
48 Food Additives: Indirect |
|
|
1 | (1) |
|
|
1 | (1) |
|
|
1 | (1) |
|
|
2 | (43) |
|
|
45 | (10) |
|
|
55 | (2) |
|
|
57 | (6) |
|
|
63 | (12) |
|
|
75 | (6) |
|
|
81 | (1) |
|
|
82 | (4) |
|
|
86 | (36) |
|
|
122 | |
|
50 Infant Formulas, Baby Foods, and Enteral Products |
|
|
1 | (1) |
|
|
1 | (83) |
|
|
84 | (42) |
|
|
126 | (8) |
|
|
134 | (4) |
|
|
138 | (11) |
|
50.6 Choline and Carnitine |
|
|
149 | (14) |
|
|
163 | (3) |
|
|
166 | (8) |
|
50.9 Sodium Monofluoroacetate |
|
|
174 | (15) |
|
50.10 Minerals and Trace Elements |
|
|
189 | (4) |
|
|
193 | (9) |
|
|
202 | (6) |
|
|
208 | (14) |
|
|
222 | (12) |
|
|
234 | (11) |
|
50.16 3-Monochloropropane-1,2-diol (MCPD) Esters and Glycidyl Esters (GE) |
|
|
245 | (13) |
|
50.17 Galactooligosaccharides (GOS) |
|
|
258 | |
|
|
1 | (1) |
|
|
1 | (9) |
|
|
10 | (3) |
|
|
13 | (5) |
|
|
18 | (5) |
|
|
23 | (3) |
|
|
26 | (3) |
|
|
29 | (2) |
|
|
31 | (2) |
|
|
33 | (4) |
|
|
37 | (5) |
|
51.11 Phosphodiesterase Type 5 Inhibitors |
|
|
42 | (4) |
|
|
46 | (1) |
|
|
47 | (4) |
|
|
51 | (3) |
|
|
54 | (3) |
|
|
57 | (9) |
|
|
66 | (1) |
|
|
67 | (2) |
|
|
69 | (2) |
|
|
71 | (3) |
|
|
74 | (6) |
|
|
80 | |
Appendix A Standard Solutions and Reference Materials |
|
1 | (1) |
Appendix B Laboratory Safety |
|
1 | (1) |
Appendix C Reference Tables |
|
1 | (1) |
Appendix D Guidelines for Collaborative Study Procedures to Validate Characteristics of a Method of Analysis |
|
1 | (1) |
Appendix E Laboratory Quality Assurance |
|
1 | (1) |
Appendix F Guidelines for Standard Method Performance Requirements |
|
1 | (1) |
Appendix G Procedures and Guidelines for the Use of AOAC Voluntary Consensus Standards to Evaluate Characteristics of a Method of Analysis |
|
1 | (1) |
Appendix H Probability of Detection (POD) as a Statistical Model for the Validation of Qualitative Methods |
|
1 | (1) |
Appendix I AOAC INTERNATIONAL Methods Committee Guidelines for Validation of Biological Threat Agent Methods and/or Procedures |
|
1 | (1) |
Appendix J AOAC INTERNATIONAL Methods Committee Guidelines for Validation of Microbiological Methods for Food and Environmental Surfaces |
|
1 | (1) |
Appendix K Guidelines for Dietary Supplements and Botanicals |
|
1 | (1) |
Appendix L AOAC Recommended Guidelines for Stakeholder Panel on Infant Formula and Adult Nutritionals (SPIFAN) Single-Laboratory Validation |
|
1 | (1) |
Appendix M Validation Procedures for Quantitative Food Allergen ELISA Methods: Community Guidance and Best Practices |
|
1 | (1) |
Appendix N ISPAM Guidelines for Validation of Qualitative Binary Chemistry Methods |
|
1 | (1) |
Appendix O Environmental Factors for Validating Biological Threat Agent Detection Assays |
|
1 | (1) |
Appendix P Guidance for Soil Collection, Characterization, and Application for Biothreat Agent Detection, Methods and Site Evaluations |
|
1 | (1) |
Appendix Q Recommendations for Developing Molecular Assays for Microbial Pathogen Detection Using Modern In Silico Approaches |
|
1 | (1) |
Appendix R Guidelines for Verifying and Documenting the Relationships Between Microbial Cultures |
|
1 | (1) |
Subject Index |
|
1 | (1) |
SMPR Index |
|
1 | (1) |
Index of Method Numbers |
|
1 | (1) |
|
Official Methods of Analysis of AOAC INTERNATIONAL |
|
|
|
|
|
|
|
List of Changes for the 22nd Edition, 2023 |
|
|
xi | |
Important Notices |
|
xvii | |
Preface |
|
xix | |
About the Association |
|
xx | |
Guide to Method Format |
|
xxiii | |
Definition of Terms and Explanatory Notes |
|
xxiv | |
AOAC® Official Methods™ Program |
|
xxxi | |
|
Standard Method Performance Requirements (SMPRs®) |
|
|
|
|
1 | (1) |
|
Stakeholder Panel on Marine and Freshwater Foods |
|
|
2 | (1) |
|
Stakeholder Panel on Agent Detection Assays |
|
|
3 | (44) |
|
Stakeholder Panel on Endocrine Disruptors |
|
|
47 | (1) |
|
Stakeholder Panel on Infant Formula and Adult Nutritionals |
|
|
48 | (53) |
|
Food--Analytical (Assorted) |
|
|
101 | (46) |
|
Stakeholder Panel on Dietary Supplements |
|
|
147 | (79) |
|
Food Microbiology/Gluten and Food Allergens |
|
|
226 | (15) |
|
AOAC Cannabis Analytical Science Program |
|
|
241 | (35) |
|
AOAC Food Authenticity Methods Program |
|
|
276 | (29) |
|
Glycerol Esters of Wood Rosin |
|
|
305 | (6) |
|
|
311 | |
|
1 Agricultural Liming Materials |
|
|
1 | (1) |
|
1.1 Liming Materials---General |
|
|
1 | (2) |
|
1.2 Calcium Silicate Slags |
|
|
3 | (1) |
|
1.3 Gravimetric Elemental Analyses |
|
|
4 | (1) |
|
1.4 Chelometric Elemental Analyses |
|
|
5 | (1) |
|
1.5 Colorimetric Elemental Analyses |
|
|
6 | |
|
|
1 | (1) |
|
2.1 Fertilizers---General |
|
|
1 | (3) |
|
|
4 | (1) |
|
|
5 | (6) |
|
|
11 | (10) |
|
|
21 | (7) |
|
|
28 | (40) |
|
|
68 | (4) |
|
|
72 | |
|
|
1 | (1) |
|
|
1 | (1) |
|
|
1 | (5) |
|
|
6 | (10) |
|
|
16 | (10) |
|
|
26 | (5) |
|
|
31 | (2) |
|
|
33 | |
|
|
1 | (1) |
|
4.1 Animal Feed---General |
|
|
1 | (22) |
|
|
23 | (12) |
|
|
35 | (1) |
|
|
36 | (3) |
|
|
39 | (4) |
|
|
43 | (11) |
|
|
54 | (12) |
|
|
66 | (10) |
|
|
76 | (2) |
|
|
78 | |
|
|
1 | (1) |
|
5.1 Feeds---General Methods |
|
|
1 | (42) |
|
5.2 Chemical Methods for Antibiotics |
|
|
43 | (15) |
|
5.3 Microbiological Methods for Antibiotics |
|
|
58 | |
|
Common and Chemical Names of Drugs |
|
|
84 | |
|
|
1 | (1) |
|
6.1 Phenol Coefficient Methods |
|
|
1 | (2) |
|
6.2 Hard Surface Carrier Test Methods |
|
|
3 | (16) |
|
|
19 | |
|
|
1 | (1) |
|
|
1 | (7) |
|
7.2 Inorganic and Organometallic Pesticides and Adjuvants |
|
|
8 | (16) |
|
|
24 | (20) |
|
|
44 | (10) |
|
7.5 Pesticides Related to Natural Products and Their Synergists |
|
|
54 | (13) |
|
7.6 Organohalogen Pesticides |
|
|
67 | (35) |
|
7.7 Thiophosphorus and Other Organophosphorus Pesticides |
|
|
102 | (25) |
|
7.8 Miscellaneous Pesticides |
|
|
127 | |
|
Common and Chemical Names of Pesticides |
|
|
136 | |
|
|
1 | (1) |
|
9 Metals and Other Elements at Trace Levels in Foods |
|
|
1 | (1) |
|
|
1 | (26) |
|
9.2 Single-Element Methods |
|
|
27 | |
|
10 Pesticide and Industrial Chemical Residues |
|
|
1 | (1) |
|
10.1 General Multiresidue Methods |
|
|
1 | (25) |
|
10.2 Organochlorine Residues |
|
|
26 | (9) |
|
10.3 Organophosphorus Residues |
|
|
35 | (14) |
|
|
49 | (1) |
|
|
49 | (7) |
|
|
56 | (39) |
|
|
95 | (34) |
|
|
129 | |
|
Common and Chemical Names of Pesticides |
|
|
132 | |
|
|
1 | (1) |
|
|
1 | (30) |
|
|
31 | |
|
|
1 | (1) |
|
|
1 | (1) |
|
14 Veterinary Analytical Toxicology |
|
|
1 | (1) |
|
|
1 | (1) |
|
|
1 | (5) |
|
15.2 Deodorants and Antiperspirants |
|
|
6 | (5) |
|
|
11 | (1) |
|
|
11 | (2) |
|
|
13 | (1) |
|
|
14 | |
|
16 Extraneous Materials: Isolation |
|
|
1 | (1) |
|
|
1 | (10) |
|
16.2 Beverages and Beverage Materials |
|
|
11 | (2) |
|
|
13 | (4) |
|
16.4 Nuts and Nut Products |
|
|
17 | (2) |
|
16.5 Grains and Their Products |
|
|
19 | (6) |
|
|
25 | (2) |
|
|
27 | (1) |
|
16.8 Eggs and Egg Products |
|
|
28 | (1) |
|
16.9 Poultry, Meat, and Fish and Other Marine Products |
|
|
29 | (3) |
|
16.10 Fruits and Fruit Products |
|
|
32 | (2) |
|
16.11 Snack Food Products |
|
|
34 | (1) |
|
16.12 Sugars and Sugar Products |
|
|
35 | (1) |
|
16.13 Vegetables and Vegetable Products |
|
|
36 | (6) |
|
16.14 Spices and Other Condiments |
|
|
42 | (10) |
|
|
52 | (7) |
|
|
59 | (10) |
|
|
69 | (3) |
|
16.18 Fruits and Fruit Products |
|
|
72 | (1) |
|
16.19 Vegetables and Vegetable Products |
|
|
73 | |
|
17 Microbiological Methods |
|
|
1 | (1) |
|
17.1 Eggs and Egg Products |
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1 | (2) |
|
17.2 Chilled, Frozen, Precooked, or Prepared Foods, and Nut Meats |
|
|
3 | (30) |
|
|
33 | (24) |
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57 | (64) |
|
|
121 | (17) |
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17.6 Sterility (Commercial) of Foods (Canned, Low Acid) |
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|
138 | (5) |
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143 | (9) |
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152 | (4) |
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156 | (138) |
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294 | (76) |
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370 | (4) |
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|
374 | (2) |
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|
376 | (1) |
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377 | (10) |
|
17.15 General Screening Methods |
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|
387 | (4) |
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|
391 | |
|
|
1 | (1) |
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|
1 | (1) |
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|
2 | (1) |
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|
3 | (2) |
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|
5 | (13) |
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18 | (3) |
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|
21 | (5) |
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|
26 | (2) |
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|
28 | (4) |
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|
32 | (12) |
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18.10 Microchemical Tests |
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|
44 | (8) |
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52 | (1) |
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53 | (1) |
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|
54 | (1) |
|
|
55 | (1) |
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|
56 | |
|
Common end Chemical Names of Drugs |
|
|
57 | |
|
|
1 | (1) |
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|
1 | (1) |
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|
2 | (5) |
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19.3 Analgesics and Antipyretics |
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7 | (5) |
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19.4 Hypnotics and Sedatives |
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12 | (8) |
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20 | (3) |
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|
23 | (3) |
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26 | (4) |
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19.8 Other Sulfur-Containing Drugs |
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|
30 | |
|
Common and Chemical Names of Drugs |
|
|
35 | |
|
|
1 | (1) |
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1 | (5) |
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6 | (1) |
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7 | (1) |
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8 | (1) |
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9 | (2) |
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11 | (3) |
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20.7 Physostigmine Alkaloids |
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14 | (2) |
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16 | (1) |
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17 | (8) |
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25 | (1) |
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26 | (3) |
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20.12 Other Natural Products |
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29 | |
|
Common and Chemical Names of Drugs |
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|
34 | |
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|
1 | (1) |
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1 | (2) |
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3 | (3) |
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21.3 Progestational Steroids |
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6 | (2) |
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21.4 Adrenocortico Steroids |
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|
8 | (6) |
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|
14 | |
|
Common and Chemical Names of Drugs |
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|
15 | |
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|
1 | (1) |
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Common and Chemical Names of Drugs |
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|
14 | |
|
23 Drugs and Feed Additives in Animal Tissues |
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|
1 | (1) |
|
Common and Chemical Names of Drugs |
|
|
78 | |
|
|
1 | (1) |
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25 Baking Powders and Baking Chemicals |
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|
1 | (1) |
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1 | (1) |
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1 | (17) |
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26.2 Cordials and Liqueurs |
|
|
18 | |
|
27 Malt Beverages and Brewing Materials |
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1 | (1) |
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1 | (21) |
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22 | (1) |
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23 | (7) |
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30 | (2) |
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32 | (3) |
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27.6 Brewing Sugars and Syrups |
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35 | (2) |
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37 | (1) |
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38 | (1) |
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|
39 | |
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1 | (1) |
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1 | (18) |
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19 | (1) |
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|
20 | |
|
29 Nonalcoholic Beverages and Concentrates |
|
|
1 | (1) |
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|
1 | (1) |
|
31 Cacao Bean and Its Products |
|
|
1 | (1) |
|
|
1 | (2) |
|
|
3 | (5) |
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|
8 | (1) |
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|
8 | (2) |
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|
10 | |
|
|
1 | (1) |
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|
1 | (45) |
|
32.2 Wheat, Rye, Oats, Corn, Buckwheat, Rice, Barley, and Soybeans and Their Products Except Cereal Adjuncts |
|
|
46 | (36) |
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|
82 | (4) |
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|
86 | (2) |
|
32.5 Macaroni, Egg Noodles, and Similar Products |
|
|
88 | |
|
|
1 | (1) |
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|
1 | (3) |
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|
4 | (66) |
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70 | (6) |
|
33.4 Evaporated and Condensed Milk |
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|
76 | (1) |
|
33.5 Dried Milk, Nonfat Dry Milk, and Malted Milk |
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|
77 | (4) |
|
|
81 | (5) |
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|
86 | (14) |
|
33.8 Ice Cream and Frozen Desserts |
|
|
100 | |
|
|
1 | (1) |
|
35 Fish and Other Marine Products |
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|
1 | (1) |
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|
1 | (1) |
|
|
1 | (1) |
|
36.2 Vanilla Extract and Its Substitutes |
|
|
1 | (14) |
|
36.3 Lemon, Orange, and Lime Extracts, Flavors, and Oils |
|
|
15 | (7) |
|
|
22 | (1) |
|
36.5 Cassia, Cinnamon, and Clove Extracts |
|
|
23 | (1) |
|
36.6 Flavor Extracts and Toilet Preparations |
|
|
24 | |
|
37 Fruits and Fruit Products |
|
|
1 | (1) |
|
38 Gelatin, Dessert Preparations, and Mixes |
|
|
1 | (1) |
|
39 Meat and Meat Products |
|
|
1 | (1) |
|
|
1 | (1) |
|
|
1 | (1) |
|
42 Vegetable Products, Processed |
|
|
1 | (1) |
|
|
1 | (8) |
|
|
9 | (1) |
|
|
10 | |
|
43 Spices and Other Condiments |
|
|
1 | (1) |
|
44 Sugars and Sugar Products |
|
|
1 | (1) |
|
|
1 | (20) |
|
44.2 Molasses and Molasses Products |
|
|
21 | (5) |
|
|
26 | (2) |
|
|
28 | (12) |
|
44.5 Maple, Sap, Maple Syrup, Maple Syrup Products |
|
|
40 | (10) |
|
|
50 | (1) |
|
44.7 Corn Syrups and Other Starch-Derived Sweeteners |
|
|
50 | |
|
45 Vitamins and Other Nutrients |
|
|
1 | (1) |
|
|
1 | (52) |
|
45.2 Microbiological Methods |
|
|
53 | (17) |
|
|
70 | (11) |
|
45.4 Nutritionally Related Components |
|
|
81 | |
|
|
1 | (1) |
|
46.1 Separation and Identification of Color Additives in Foods, Drugs, and Cosmetics |
|
|
1 | (12) |
|
|
13 | (7) |
|
46.3 Subsidiary and Lower Sulfonated Dyes |
|
|
20 | (1) |
|
46.4 Metals and Other Elements |
|
|
21 | (3) |
|
|
24 | (1) |
|
|
25 | |
|
47 Food Additives: Direct |
|
|
1 | (1) |
|
|
1 | (1) |
|
|
1 | (15) |
|
47.3 Chemical Preservatives |
|
|
16 | (30) |
|
|
46 | (1) |
|
|
47 | (2) |
|
|
49 | |
|
48 Food Additives: Indirect |
|
|
1 | (1) |
|
|
1 | (1) |
|
|
1 | (1) |
|
|
2 | (43) |
|
|
45 | (10) |
|
|
55 | (2) |
|
|
57 | (6) |
|
|
63 | (12) |
|
|
75 | (6) |
|
|
81 | (1) |
|
|
82 | (4) |
|
|
86 | (36) |
|
|
122 | |
|
50 Infant Formulas, Baby Foods, and Enteral Products |
|
|
1 | (1) |
|
|
1 | (83) |
|
|
84 | (42) |
|
|
126 | (8) |
|
|
134 | (4) |
|
|
138 | (11) |
|
50.6 Choline and Carnitine |
|
|
149 | (14) |
|
|
163 | (3) |
|
|
166 | (8) |
|
50.9 Sodium Monofluoroacetate |
|
|
174 | (15) |
|
50.10 Minerals and Trace Elements |
|
|
189 | (4) |
|
|
193 | (9) |
|
|
202 | (6) |
|
|
208 | (14) |
|
|
222 | (12) |
|
|
234 | (11) |
|
50.16 3-Monochloropropane-1,2-diol (MCPD) Esters and Glycidyl Esters (GE) |
|
|
245 | (13) |
|
50.17 Galactooligosaccharides (GOS) |
|
|
258 | |
|
|
1 | (1) |
|
|
1 | (9) |
|
|
10 | (3) |
|
|
13 | (5) |
|
|
18 | (5) |
|
|
23 | (3) |
|
|
26 | (3) |
|
|
29 | (2) |
|
|
31 | (2) |
|
|
33 | (4) |
|
|
37 | (5) |
|
51.11 Phosphodiesterase Type 5 Inhibitors |
|
|
42 | (4) |
|
|
46 | (1) |
|
|
47 | (4) |
|
|
51 | (3) |
|
|
54 | (3) |
|
|
57 | (9) |
|
|
66 | (1) |
|
|
67 | (2) |
|
|
69 | (2) |
|
|
71 | (3) |
|
|
74 | (6) |
|
|
80 | |
Appendix A Standard Solutions and Reference Materials |
|
1 | (1) |
Appendix B Laboratory Safety |
|
1 | (1) |
Appendix C Reference Tables |
|
1 | |