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El. knyga: Phage Display: Methods and Protocols

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  • Formatas: PDF+DRM
  • Serija: Methods in Molecular Biology 2702
  • Išleidimo metai: 07-Sep-2023
  • Leidėjas: Springer-Verlag New York Inc.
  • Kalba: eng
  • ISBN-13: 9781071633816
Kitos knygos pagal šią temą:
Phage Display: Methods and ProtocolsDidesnis paveikslėlis
  • Formatas: PDF+DRM
  • Serija: Methods in Molecular Biology 2702
  • Išleidimo metai: 07-Sep-2023
  • Leidėjas: Springer-Verlag New York Inc.
  • Kalba: eng
  • ISBN-13: 9781071633816
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This second edition details new and updated methods on different antibody libraries, along with novel approaches for antibody discovery. Chapters focuses on the construction of antibody libraries, antibody expression, complementary approaches for antibody selection, and other phage display related applications. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.





 





Authoritative and cutting-edge, Phage Display: Methods and Protocols, Second Edition aims to be a useful and practical guide to new researchers and experts looking to expand their knowledge. 





 
Antibody phage display.- Construction of human immune and naive scFv
Phage Display libraries.- Construction of Naļve and Immune Human Fab Phage
Display Library.- Construction of synthetic antibody phage display
libraries.- Construction of chicken and ostrich antibody libraries.-
Construction of Rabbit Immune Antibody Libraries.- Isolation and
characterization of single-domain antibodies from immune phage display
libraries.- Phagekines: directed evolution and characterization of functional
cytokines displayed on phages.- Efficient Cloning of Inserts for Phage
Display by Golden Gate Assembly.- Construction of an ultra-large phage
display library by Kunkel mutagenesis and rolling circle
amplification.- Construction of semi-synthetic shark vNAR yeast surface
display antibody libraries.- Antibody selection via Phage Display in
microtiter plates.- Antibody selection in solution using magnetic
beads.- Streptavidin Coated Solid Phase Extraction (SPE) TipsFor Antibody
Phage Display Biopanning.- Magnetic Nanoparticle-Based Semi-Automated Panning
for High-throughput Antibody Selection.- Antibody selection on cells
targeting membrane proteinsTargeting Intracellular Antigens With pMHC-binding
Antibodies: A Phage Display Approach.- Antibody Isolation from a Human
Synthetic Libraries of Single-chain Antibodies and analysis using
NGS.- Selection of affibody affinity proteins from phagemid libraries.-
Antibody affinity and stability maturation by error-prone PCR.- Antibody
batch cloning.- Deep Mining of Complex Antibody Phage Pools.- High-throughput
IgG reformatting and expression using hybrid secretion signals and InTag
positive selection technology.- Validation and the Determination of Antibody
Bioactivity Using MILKSHAKE and Sundae Protocols.- Mapping polyclonal
antibody responses to infection using next generation phage
display.- Applications of high-throughput DNA sequencing to single-domain
antibodydiscovery and engineering.- Biomarker discovery by ORFeome Phage
Display.- Mapping Epitopes by Phage Display.- Epivolve: A Protocol for
Site-directed Antibodies.
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