| Abbreviations |
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viii | (1) |
| Preface |
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ix | |
| PART 1: BASIC PRINCIPLES AND METHODS |
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1 | (58) |
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1 | (6) |
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1 | (4) |
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Advantages of tissue cultures over intact plants |
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5 | (2) |
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2. Equipment and general practise |
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7 | (6) |
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Conditions for media preparation |
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7 | (3) |
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Sterile cabinets or rooms for aseptic transfer |
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10 | (1) |
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Temperature and lighting requirements for growth of cultures |
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11 | (2) |
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3. Aseptic techniques in plant tissue culture |
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13 | (6) |
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Preparation of the laminar flow cabinet |
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13 | (1) |
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Handling pipettes and pipette tips |
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14 | (1) |
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Aseptic transfer of media |
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14 | (1) |
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Aseptic transfer of callus and cell suspensions |
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15 | (4) |
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4. Preparation of plant tissue culture media |
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19 | (8) |
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19 | (4) |
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23 | (1) |
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Preparation and sterilization of media |
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24 | (3) |
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5. Initiation of callus cultures |
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27 | (8) |
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Source of explants for plant cultures |
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28 | (1) |
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Sterilization methods to remove surface contaminants |
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28 | (3) |
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Initiation of callus from explants |
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31 | (3) |
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Presence of endogenous contaminants |
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34 | (1) |
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6. Growth of callus and cell suspension cultures |
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35 | (10) |
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Maintenance of callus cultures |
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35 | (3) |
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Initiation of cell suspension cultures |
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38 | (7) |
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7. Regeneration of tissue cultures |
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45 | (14) |
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Development of somatic embryos |
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45 | (1) |
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Induction of regeneration in tissue cultures |
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46 | (8) |
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Decline in totipotency in tissue cultures |
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54 | (1) |
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Transfer of tissue culture-derived plants to soil |
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55 | (1) |
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Markers for regenerating potential |
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56 | (3) |
| PART 2: APPLICATIONS |
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59 | (92) |
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59 | (12) |
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61 | (3) |
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64 | (1) |
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Interspecific hybridization using excised embryos |
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65 | (2) |
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Doubling of chromosome numbers |
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67 | (1) |
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Comparison of methods of dihaploid production |
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68 | (1) |
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Genetic variation in dihaploids |
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69 | (2) |
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71 | (12) |
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Protoplast isolation and culture |
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72 | (4) |
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Methods of protoplast fusion |
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76 | (3) |
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Selection of heterokaryons |
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79 | (1) |
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Contribution of protoplast fusion to crop breeding |
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80 | (3) |
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10. Preservation and cryopreservation of germplasm |
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83 | (8) |
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Reduction in growth rates of cells and tissues |
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84 | (1) |
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Inhibition of growth of cells and tissues by cryopreservation |
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85 | (4) |
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Confirmation of viability and stability of cryopreserved tissues |
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89 | (2) |
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11. Selection for somaclonal variants |
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91 | (12) |
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Source of somaclonal variation |
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92 | (3) |
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95 | (6) |
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Progeny testing of somaclones |
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101 | (1) |
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Application of somaclonal variation to crop improvement |
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101 | (2) |
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12. Secondary product synthesis by plant tissue cultures |
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103 | (18) |
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Effect of culture conditions on secondary product synthesis |
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105 | (8) |
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Cell selection in vitro for high yielding cell lines |
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113 | (2) |
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Genetic modification of tissue cultures |
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115 | (2) |
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Large-scale culture of plant cells |
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117 | (4) |
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13. Micropropagation techniques in horticulture and crop improvement |
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121 | (18) |
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Source tissue for micropropagation |
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123 | (1) |
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Methods of micropropagation |
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124 | (4) |
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Problems that arise in vitro during micropropagation |
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128 | (3) |
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Problems that arise in vivo after micropropagation |
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131 | (2) |
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Variation in clonal plants |
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133 | (1) |
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Advantages and disadvantages of micropropagation |
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134 | (5) |
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14. Genetic manipulation of crop plants |
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139 | (12) |
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Strategy for plant transformation through gene transfer |
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140 | (2) |
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Gene manipulation with A.tumefaciens |
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142 | (3) |
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Non-tissue culture based transformation using A.tumefaciens |
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145 | (1) |
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Tissue culture transformation not based on A.tumefaciens |
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146 | (2) |
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Genetically engineered characters in crop plants |
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148 | (3) |
| Appendices |
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151 | (4) |
| Appendix A: Sources and suppliers for chemicals and equipment |
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151 | (2) |
| Appendix B: Further reading |
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153 | (2) |
| Index |
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155 | |
