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Plant Circadian Networks: Methods and Protocols Second Edition 2022 [Minkštas viršelis]

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  • Formatas: Paperback / softback, 245 pages, aukštis x plotis: 254x178 mm, weight: 500 g, 33 Illustrations, color; 17 Illustrations, black and white; XI, 245 p. 50 illus., 33 illus. in color., 1 Paperback / softback
  • Serija: Methods in Molecular Biology 2398
  • Išleidimo metai: 23-Oct-2022
  • Leidėjas: Springer-Verlag New York Inc.
  • ISBN-10: 1071619144
  • ISBN-13: 9781071619148
Kitos knygos pagal šią temą:
Plant Circadian Networks: Methods and Protocols Second Edition 2022Didesnis paveikslėlis
  • Formatas: Paperback / softback, 245 pages, aukštis x plotis: 254x178 mm, weight: 500 g, 33 Illustrations, color; 17 Illustrations, black and white; XI, 245 p. 50 illus., 33 illus. in color., 1 Paperback / softback
  • Serija: Methods in Molecular Biology 2398
  • Išleidimo metai: 23-Oct-2022
  • Leidėjas: Springer-Verlag New York Inc.
  • ISBN-10: 1071619144
  • ISBN-13: 9781071619148
Kitos knygos pagal šią temą:
Pastovi nuoroda: https://www.kriso.lt/db/9781071619148.html
Raktažodžiai:
This second edition provides new and updated protocols to investigate clock-controlled parameters including period estimations and entrainment measurements and modelling. Chapters detail promoter activity using luciferase reporters, fluorescent-tagging of clock proteins and cellular imaging, protein level measurements, regulated RNA splicing detection, post-translational modifications, protein-protein interactions, and protein DNA/chromatin interactions. Additional chapters focus on seasonal processes like hypocotyl growth and flowering time control in annuals and trees, and techniques on soybean. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols.





Authoritative and practical, Plant Circadian Networks: Methods and Protocols, Second Edition is designed not only for the chronobiology community dealing with circadian biology but also for the plant community in general. 





Period estimation and rhythm detection in timeseries data using BioDare2, the free, online, community resource is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.
Circadian Rhythm: Phase Response Curve and Light Entrainment.- Period
Estimation and Rhythm Detection in Timeseries Data Using BioDare2, the Free,
Online, Community Resource.- Detection and Analysis of Circadian Rhythms Via
Prompt Chlorophyll Fluorescence.- High Spatial Resolution Luciferase Imaging
of the Arabidopsis thaliana Circadian Clock.- Agrobacterium-mediated seedling
transformation to measure circadian rhythms in Arabidopsis.- Measurement of
Luciferase Rhythms in Soybean Hairy Roots.-ODE (ordinary differential
equation) Models for Plant Circadian Networks: What The Models Are, and How
They Should Be Used.- How to Detect QTLs in the Plant Circadian
Clock.- Measuring Hypocotyl Length in Arabidopsis.- High Throughput
Extraction and Enzymatic Determination of Sugars and Fructans In
Fructan-Accumulating Plants.- Simple Nuclei Preparation and
Co-Immunoprecipitation Procedures for Studying Protein Abundance and
Interactions In Plant Circadian Time Courses.- Chromatin Immunoprecipitation
Protocol for Circadian Clock Proteins.- A high-throughput 3-Tag RNA
Sequencing for Large-Scale Time-Series Transcriptome Studies.- Experimental
Design for Time-Series RNA-seq Analysis of Gene Expression and Alternative
Splicing.- Using tandem affinity purification to identify circadian clock
protein complexes from Arabidopsis.- Firefly Luciferase Complementation-Based
Analysis of Dynamic Protein-Protein Interactions under Diurnal and Circadian
Conditions in Arabidopsis.- Monitoring Seasonal Bud Set, Bud Burst, and Cold
Hardiness in Populus.- The Perennial Clock Is an Essential Timer for Seasonal
Growth Events and Cold Hardiness.