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El. knyga: Plant Embryogenesis: Methods and Protocols

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  • Formatas: PDF+DRM
  • Serija: Methods in Molecular Biology 2122
  • Išleidimo metai: 23-Jan-2020
  • Leidėjas: Springer-Verlag New York Inc.
  • Kalba: eng
  • ISBN-13: 9781071603420
Kitos knygos pagal šią temą:
Plant Embryogenesis: Methods and ProtocolsDidesnis paveikslėlis
  • Formatas: PDF+DRM
  • Serija: Methods in Molecular Biology 2122
  • Išleidimo metai: 23-Jan-2020
  • Leidėjas: Springer-Verlag New York Inc.
  • Kalba: eng
  • ISBN-13: 9781071603420
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This volume details state-of-the-art methods for the study of plant embryogenesis in the model organism Arabidopsis thaliana, other models, and non-model species. Chapters guide readers through genetic screens, phenotypic analysis, live imaging, transcriptional profiling, methods on other model and non-model species beyond Arabidopsis thaliana, and introduction to systems that allow to culture or produce zygotic and somatic embryos in vitro. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Plant Embryogenesis: Methods and Protocols aims to ensure successful results in the further study of this vital field.

The chapter “Small RNA In Situ Hybridizations on Sections of Arabidopsis Embryos” is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.


Genetic Screens to Target Embryo and Endosperm Pathways in Arabidopsis
and Maize.- EMS Mutagenesis of Arabidopsis Seeds.- EMS Mutagenesis of Maize
Pollen.- Live-cell Imaging of Zygotic Intracellular Structures and Early
Embryo Pattern Formation in Arabidopsis thaliana.- Analyzing Sub-Cellular
Reorganization during Early Arabidopsis Embryogenesis using Fluorescent
Markers.- From Stained Plant Tissues to Quantitative Cell Segmentation
Analysis with MorphoGraphX.- Small RNA In Situ Hybridizations on Sections of
Arabidopsis Embryos.- Manual Isolation of Living Early Embryos from Tobacco
Seeds.- Profiling Transcriptomes of Manually Dissected Arabidopsis
Embryos.- Laser-assisted Microdissection of Plant Embryos for Transcriptional
Profiling.- Cell-type-specific Transcriptomics in the Plant Embryo Using an
Adapted INTACT Protocol.- Transcriptomic Profiling of the Arabidopsis
Embryonic Epidermis UsingFANS in Combination with RNAseq.- Visualizing
Protein Associations in living Arabidopsis Embryo.- Imaging of Embryo Sac and
Early Seed Development in Maize after Feulgen Staining.- Using Giant Scarlet
Runner Bean (Phaseolus coccineus) Embryos to Dissect the Early Events in
Plant Embryogenesis.- Microscopical Detection of Cell Death Processes during
Scots Pine Zygotic Embryogenesis.- Regulation of Somatic Embryo Development
in Norway Spruce.- In vitro Production of Zygotes by Electro-Fusion of Rice
Gametes.- Isolated Microspore Culture in Brassica napus.- Anther Culture in
Eggplant (Solanum melongena L.). 








 
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