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El. knyga: Plant Functional Genomics: Methods and Protocols, Volume 1

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  • Formatas: EPUB+DRM
  • Serija: Methods in Molecular Biology 2787
  • Išleidimo metai: 24-Apr-2024
  • Leidėjas: Springer-Verlag New York Inc.
  • Kalba: eng
  • ISBN-13: 9781071637784
Kitos knygos pagal šią temą:
Plant Functional Genomics: Methods and Protocols, Volume 1 Didesnis paveikslėlis
  • Formatas: EPUB+DRM
  • Serija: Methods in Molecular Biology 2787
  • Išleidimo metai: 24-Apr-2024
  • Leidėjas: Springer-Verlag New York Inc.
  • Kalba: eng
  • ISBN-13: 9781071637784
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This detailed book gathers hands-on techniques to characterize, identify, localize, profile, and determine genome functions in plants. Volume 1 opens with a section exploring protocols to advance our understanding of plant genetics and ecology via phenomics, and it continues with chapters on plant genetics, transcriptomics, and proteomics to unravel plant protein structures and functions, bridging proteomics and phosphoproteomics. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and comprehensive, Plant Functional Genomics: Methods and Protocols serves as an ideal guide to the immense promise of functional genomics, spanning from fundamental evolutionary studies to practical advancements in crop improvement.
An Overview of High-Throughput Crop Phenotyping: Platform, Image
Analysis, Data Mining, and Data Management.- A High-Throughput Approach for
Photosynthesis Studies in a Brassicaceae Panel.- Methods for Functional
Physiological Phenotyping and High-Order Data Quantification.- Physiotyping
of Plants and Modeling the Soil Plant Atmospheric Continuum (SPAC).- Root
Hair Imaging Using Confocal Microscopy.- Cytogenetic Bioindication in Root
Meristems for Vitality Assessment of Trees.- Genetic Diversity Assessment in
Plants from Reduced Representation Sequencing Data.- Identification of Novel
Induced Mutations in Seed and Vegetatively Propagated Plants from Reduced
Representation or Whole Genome Sequencing Data.- Identification of Induced
Copy Number Variation from Low Coverage Sequence Data.- Genetic Map
Construction Using F2 and RIL/DH Mapping Population.- QTL Mapping Using RIL
Population.- Sample and Library Preparation for PacBio Long Read Sequencing
in Grapevine.- RNA Isolation Method in Marginal Crops with High Agronomic
Potential.- A General Protocol for Gene Expression Analysis in Chemically
Mutant Coffee Plants in Response to Rust (Hemileia vastatrix Berk & Broome)
Infection.- Transcriptomic Data Analysis Using the Galaxy Platform: Coffee
(Coffea arabica L.) Flowers as Example.- Quantitative Estimation of Promoter
Activity in Cannabis sativa Using Agroinfiltration-Based Transient Gene
Expression.- Indophenol Blue Colorimetric Method to Determine Grain Protein
Content of Cereal Plants.- One-Dimensional Acrylamide Gel Electrophoresis for
Analysis of Plant Samples.- Two-Dimensional Gel Electrophoresis for
Separation of Plant Samples.- Sample Preparation and Phosphopeptide
Enrichment for Plant Phosphoproteomics via Label-Free Mass Spectrometry.-
Protein-Protein Interactions Visualized by Bimolecular Fluorescence
Complementation in Arabidopsis thaliana Protoplasts from Leaf.- Determining
Macromolecular Structures Using Cryo-Electron Microscopy.- Determining
Protein Structures Using X-Ray Crystallography.
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