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Plant Functional Genomics: Methods and Protocols, Volume 2 2024 ed. [Kietas viršelis]

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  • Formatas: Hardback, 413 pages, aukštis x plotis: 254x178 mm, 1 Illustrations, black and white; XIV, 413 p. 1 illus., 1 Hardback
  • Serija: Methods in Molecular Biology 2788
  • Išleidimo metai: 25-Apr-2024
  • Leidėjas: Springer-Verlag New York Inc.
  • ISBN-10: 1071637819
  • ISBN-13: 9781071637814
Kitos knygos pagal šią temą:
  • Formatas: Hardback, 413 pages, aukštis x plotis: 254x178 mm, 1 Illustrations, black and white; XIV, 413 p. 1 illus., 1 Hardback
  • Serija: Methods in Molecular Biology 2788
  • Išleidimo metai: 25-Apr-2024
  • Leidėjas: Springer-Verlag New York Inc.
  • ISBN-10: 1071637819
  • ISBN-13: 9781071637814
Kitos knygos pagal šią temą:
This detailed book gathers hands-on techniques to characterize, identify, localize, profile, and determine genome functions in plants. Volume 2 opens with metabolomic methods that uncover chemical intricacies, exploring glycome mixtures, cell wall components, and computational metabolomics, and it continues with sections on plant genome validation, computational approaches and bioinformatic tools, and case studies exploring the biological effects of magnetic fields and the study of cyanobacteria. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 





 





Authoritative and comprehensive, Plant Functional Genomics: Methods and Protocols serves as an ideal guide to the immense promise of functional genomics, spanning from fundamental evolutionary studies to practical advancements in crop improvement.





Chapter 7 is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.
Carotenoid Extraction from Plant Tissues.- Sample Preparation from Plant
Tissue for Gas ChromatographyMass Spectrometry (GCMS).- Identification of
Volatiles in Tomato Fruit Using Headspace Solid-Phase-Micro-Extraction
(HS-SPME) Coupled with Gas Chromatography-Mass Spectrometry (GC-MS).-
Determination of Molecular Dimensions of Carbohydrate Polymers
(Polysaccharides) by Size Exclusion Chromatography (SEC).- Monosaccharide
Analysis after One-Pot Derivatization Followed by Reverse-Phase Liquid
Chromatography Separation and UV/Vis Detection.- Atomic Force Microscopy in
the Characterization of the Structure of Cell Wall Components.- Studying
Plant Specialized Metabolites Using Computational Metabolomics Strategies.-
pyPGCF: A Python Software for Phylogenomic Analysis, Species Demarcation,
Identification of Core and Fingerprint Proteins of Bacterial Genomes that Are
Important for Plants.- Application of Integrated Computational Approaches in
Prediction of Plant Virus Encoded miRNAs and Their Targeted Plant Genes.-
Gene Co-Expression Network Analysis to Mine Genes Involved in Plant Secondary
Metabolite Biosynthesis and Regulation.- Organic Supplementation of Vaccinium
corymbosum Micropropagation Media.- Coffee Cell Suspensions as a Platform for
Transient Gene Expression Analysis.- Agroinfiltration for Enhanced Transgene
Expression in Coffee Leaves (Coffea arabica L.).- In Vitro Gamma Mutagenesis
Techniques in Rice (Oryza sativa L. var. Lazarroz FL).- CRISPR/Cas9-Mediated
Genome Editing in Indica Rice (Oryza sativa L. subsp. indica var.
CR-5272).- Dual-Luciferase Reporter Assay for Pre-Screening CRISPR
(d)Cas9-Mediated Epigenetic Editing on a Plant Promoter Using Human Cells.-
In Silico Design of gRNA for CRISPR/Cas9-Mediated Gene Knock-Out.-
CRISPR/Cas9 Vector Construction for Gene Knock-Out.- A Comprehensive Protocol
for Assembly of Multiple gRNAs into a Direct Vector for Genome Editing in
Tomato.- Genome Editing in Brassica juncea Using CRISPR/Cas9 Technology.-
Precision Genome Editing with CRISPR-Cas9.- Using Magnetic Fields to Enhance
the Seed Germination, Growth, and Yield of Plants.- Phenotypic and Genotypic
Methods for the Identification and Quantification of Cyanobacteria in Lake
Water.