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Plant Genome Engineering: Methods and Protocols 1st ed. 2023 [Kietas viršelis]

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  • Formatas: Hardback, 388 pages, aukštis x plotis: 254x178 mm, weight: 954 g, 65 Illustrations, color; 5 Illustrations, black and white; XV, 388 p. 70 illus., 65 illus. in color., 1 Hardback
  • Serija: Methods in Molecular Biology 2653
  • Išleidimo metai: 31-Mar-2023
  • Leidėjas: Springer-Verlag New York Inc.
  • ISBN-10: 1071631306
  • ISBN-13: 9781071631300
Kitos knygos pagal šią temą:
Plant Genome Engineering: Methods and Protocols 1st ed. 2023Didesnis paveikslėlis
  • Formatas: Hardback, 388 pages, aukštis x plotis: 254x178 mm, weight: 954 g, 65 Illustrations, color; 5 Illustrations, black and white; XV, 388 p. 70 illus., 65 illus. in color., 1 Hardback
  • Serija: Methods in Molecular Biology 2653
  • Išleidimo metai: 31-Mar-2023
  • Leidėjas: Springer-Verlag New York Inc.
  • ISBN-10: 1071631306
  • ISBN-13: 9781071631300
Kitos knygos pagal šią temą:
Pastovi nuoroda: https://www.kriso.lt/db/9781071631300.html
Raktažodžiai:

This volume provides readers with a collection of the latest protocols used to study plant genome editing and trait engineering. The chapters in this book are organized into five parts: genome engineering systems; machinery design and validation; delivery tools; generation and analysis of engineering materials; and crop genome engineering applications. The chapters cover topics such as methods of applying the popular CRISPR-Cas9 or CRISPR-Cas12 systems for editing genomes in different crop species, the use of small synthetic plastome for potato genome engineering, and the use of CRISPR-Cas9 for algal cell genome engineering. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and comprehensive, Plant Genome Engineering: Methods and Protocols is a valuable tool for researchers interested in learning more about this developing and important field.


PAM-Less CRISPR-SpRY Genome Editing in Plants.- Type I-D CRISPR
System-Mediated Genome Editing in Plants.- CRISPR/LbCas12a-Mediated Genome
Editing in Soybean.- Base Editing in Poplar through an Agrobacterium-Mediated
Transformation Method.- Genetic Engineering of Potato (Solanum tuberosum)
Chloroplasts using the Small Synthetic Plastome
Mini-Synplastome.- Designing Guide-RNA for Generating Premature Stop Codons
for Gene Knockout using CRISPR-BETS.- Construction of CRISPR/Cas9 Multiplex
Genome Editing System in Rice.- Use of Fluorescent Protein Reporters for
Assessing and Detecting Genome Editing Reagents and Transgene Expression in
Plants.- Automated, High-Throughput Protoplast Transfection for Gene Editing
and Transgene Expression Studies.- Delivery of CRISPR-Cas12a
Ribonucleoprotein Complex for Genome Editing in an Embryogenic Citrus Cell
Line.- Transgene-Free Genome Editing in Nicotiana benthamiana with
CRISPR/Cas9 Delivered by a Rhabdovirus Vector .- Ribonucleoprotein
(RNP)-Mediated Targeted Mutagenesis in Barley (Hordeum vulgare
L.).- Ribonucleoprotein (RNP)-Mediated Allele Replacement in Barley (Hordeum
vulgare L.) Leaves.- Genome Editing in Chlamydomonas reinhardtii Using
Cas9-gRNA Ribonucleoprotein Complex: A Step-by-Step Guide.- Highly Efficient
Gene Knockout in Medicago truncatula Genotype R108 using CRISPR-Cas9 System
and an Optimized Agrobacterium Transformation Method.- Efficient Targeted
Mutagenesis in Brassica Crops using CRISPR/Cas Systems.- Introduction of
Genome Editing Reagents and Genotyping of Derived Edited Alleles in Soybean
(Glycine max (L.) Merr.).- A CRISPR/Cas9 Protocol for Target Gene Editing in
Barley.- Targeted Insertion in Nicotiana benthamiana Genomes via Protoplast
Regeneration.- Stepwise Optimization of Real-Time RT-PCR
Analysis.- CRISPR/Cas9 Technology for Potato Functional Genomics and
Breeding.- Recent Advances in Engineering of In Vivo Haploid Induction
Systems.