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El. knyga: Plant Transformation via Agrobacterium Tumefaciens: Culture Conditions, Recalcitrance and Advances in Soybean [Taylor & Francis e-book]

  • Formatas: 226 pages, 17 Tables, black and white; 2 Line drawings, color; 1 Line drawings, black and white; 27 Halftones, color; 1 Halftones, black and white; 29 Illustrations, color; 2 Illustrations, black and white
  • Išleidimo metai: 02-Sep-2022
  • Leidėjas: CRC Press
  • ISBN-13: 9781003281245
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  • Taylor & Francis e-book
  • Kaina: 267,74 €*
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Plant Transformation via Agrobacterium Tumefaciens: Culture Conditions, Recalcitrance and Advances in SoybeanDidesnis paveikslėlis
  • Formatas: 226 pages, 17 Tables, black and white; 2 Line drawings, color; 1 Line drawings, black and white; 27 Halftones, color; 1 Halftones, black and white; 29 Illustrations, color; 2 Illustrations, black and white
  • Išleidimo metai: 02-Sep-2022
  • Leidėjas: CRC Press
  • ISBN-13: 9781003281245
Kitos knygos pagal šią temą:
Taylor & Francis e-booksMore info about Taylor & Francis e-books
Partnerių interneto svetainė: https://www.taylorfrancis.com/books/9781003281245
Plant Transformation via Agrobacterium Tumefaciens compiles fundamental and specific information and procedures involving in vitro soybean transformation, which forms the basis for the Agrobacterium-mediated genetic manipulation of soybean using plant tissue culture. This method serves as one of the most preferred, reliable and cost-effective mechanism of transgene expression in both leguminous recalcitrant species and non-legume crops. The technology is favoured due to its simplicity, feasibility and high transformation rates that are so far achieved mostly in monocot plants and a few dicot genotypes.

This book provides a comprehensive review of plant transformation which remains necessary for many researchers who are still facing protocol-related hurdles. Among some of the major topics covered in Plant Transformation via Agrobacterium Tumefaciens are the history and discovery of Agrobacterium bacterium, longstanding challenges causing transformation inefficiencies, types and conditions of explants, development of transgenic plants for stress resistance, and the role of transgenic plants on animal/human health, including the environment.

Plant Transformation via Agrobacterium Tumefaciens helps the reader to understand how soybean, like many other orphan legume crops, faces the risk of overexploitation which may render the currently available varieties redundant and extinct should its narrow gene pool not improve. Plant transformation serves as a key technique in improving the gene pool, while developing varieties that are drought tolerant, have enhanced nutritional value, pest resistant and reduce the destruction by disease causing microorganims. This book is an essential foundation tool that is available for researchers and students to reinforce the application of Agrobacterium-mediated genetic transformation in soybean.
Preface xiii
Acknowledgements xv
Author xvii
Chapter 1 An Overview of Genetic Transformation in Plants
1(10)
1.1 Introduction
1(2)
1.2 Molecular Mechanism for DNA Introgression
3(1)
1.3 Tissue Targeting for Plant Transformation
4(1)
1.4 Screening and Selection of Transformants
5(2)
1.5 Transfer to Natural Environment
7(2)
1.6 Summary
9(1)
1.7 Abbreviations
9(2)
References
10(1)
Chapter 2 Plant Transformation History
11(14)
2.1 Discovery of Agrobacterium tumefaciens
11(1)
2.2 Tumour-Inducing Principle
12(2)
2.3 Structure and Modifications of the Agrobacterium Circular DNA
14(2)
2.4 Genetic Transformation in Plants
16(1)
2.5 The First Transgenic Plant
17(2)
2.6 Role of Plant Tissue Culture
19(2)
2.7 Summary
21(1)
2.8 Abbreviations
22(3)
References
22(3)
Chapter 3 Agrobacterium tumefaciens
25(14)
3.1 Introduction
25(1)
3.2 Classification
25(2)
3.3 General Characteristics of Agrobacterium
27(2)
3.4 Size and Morphology
29(1)
3.5 Nutrition
30(1)
3.6 Reproduction
31(1)
3.7 Cell Structure
32(1)
3.8 Protoplasmic Materials
33(1)
3.9 Chromosomal DNA and Plasmids
34(1)
3.10 Summary
35(1)
3.11 Abbreviations
35(4)
References
36(3)
Chapter 4 Getting Started with in Vitro Soybean Transformation
39(20)
4.1 Introduction
39(1)
4.2 Bacterial Strains
40(2)
4.2.1 Bacterial Culture
41(1)
4.3 Establishing Contamination-Free Cultures
42(4)
4.3.1 Aseptic Seed Cultures
43(2)
4.3.2 Culture Medium
45(1)
4.4 Type and Competency of Explants
46(1)
4.5 Co-Cultivation of Explants with A. tumefaciens
47(1)
4.6 Shoot Induction and Elongation
48(1)
4.7 Rooting of Elongated Shoots
49(1)
4.8 Acclimatisation
49(1)
4.9 Screening of Transgenic Plants
50(1)
4.10 Other Considerations
51(2)
4.10.1 Equipment and Laboratory Supplies
52(1)
4.10.2 Surfactants
52(1)
4.10.3 Antinecrotic Treatments
53(1)
4.11 Summary
53(1)
4.12 Abbreviations
54(5)
References
55(4)
Chapter 5 In Vitro Cultures Commonly Used for Plant Transformation
59(20)
5.1 Introduction
59(1)
5.2 Callus Culture
60(3)
5.2.1 Purpose of Callus Culture
60(2)
5.2.2 Requirements for Establishing Callus Culture
62(1)
5.2.3 Application in Plant Transformation
63(1)
5.3 Shoot Culture
63(3)
5.3.1 Purpose of Shoot Culture
64(1)
5.3.2 Culture Requirements for Efficient Shoot Multiplication
65(1)
5.3.3 Application of Shoot Culture in Plant Transformation
66(1)
5.4 Meristem Culture
66(4)
5.4.1 Purpose of Meristem Culture
68(1)
5.4.2 Meristem Culture Requirements
69(1)
5.4.3 Use of Meristem Culture for Plant Improvement
69(1)
5.5 Protoplast Culture
70(3)
5.5.1 Purpose of Protoplast Culture
71(1)
5.5.2 Requirements for Protoplast Culture
72(1)
5.5.3 Applications in Plant Transformation
73(1)
5.6 Summary
73(1)
5.7 Abbreviations
74(5)
References
75(4)
Chapter 6 Current and Longstanding Challenges Facing Soybean Transformation
79(16)
6.1 Introduction
79(1)
6.2 Longstanding Factors Influencing Soybean Transformation
80(5)
6.2.1 Genotype Competency and Regenerability
80(1)
6.2.2 Choice of Explants
81(1)
6.2.3 Co-Cultivation Conditions
82(1)
6.2.4 Selection System
83(1)
6.2.5 Genetic or Chimeric Effects
84(1)
6.3 Emerging Factors Affecting Soybean Transformation
85(5)
6.3.1 Seed Quality
85(1)
6.3.2 Seasonality
86(1)
6.3.3 Sterilisation Techniques
87(1)
6.3.4 Plant Growth Regulators
88(1)
6.3.5 Antibiotics
88(1)
6.3.6 Oxidative Stress
89(1)
6.4 Summary
90(1)
6.5 Abbreviations
91(4)
References
91(4)
Chapter 7 Alternative Techniques for Genetic Manipulations in Soybean
95(18)
7.1 Introduction
95(1)
7.2 Alternative Techniques for Genetic Improvement
95(7)
7.2.1 Particle Bombardment/Biolistic Method
96(1)
7.2.2 Liposome-Mediated Transfection
97(1)
7.2.3 Fibre-Mediated DNA Delivery
98(1)
7.2.4 Laser-Induced Genetic Improvement
99(1)
7.2.5 In-Planta Transformations
99(1)
7.2.6 Mutagenesis
100(1)
7.2.7 Marker-Assisted Selection
100(1)
7.2.8 Qualitative Trait Loci
101(1)
7.2.9 CRISPR-Cas9
102(1)
7.3 Other Uncommon Methods
102(2)
7.3.1 Transfection via Calcium Phosphate Precipitation
103(1)
7.3.2 Diethyl Amino Ethyl (DEAE) Dextran-Mediated DNA Transfer
103(1)
7.3.3 Polyethylene Glycol (PEG)-Mediated DNA Transfer
104(1)
7.4 Status of A. rhizogenes in Plant Transformation
104(1)
7.5 Pros and Cons of Artificial Gene Transfer Methods
105(1)
7.6 Summary
106(1)
7.7 Abbreviations
107(6)
References
108(5)
Chapter 8 Molecular Aspects of Indirect Gene Transfer via A. tumefaciens
113(14)
8.1 Introduction
113(1)
8.2 Molecular Mechanism of Agrobacterium-Mediated Gene Transfer
114(4)
8.2.1 Pathogenesis
114(1)
8.2.2 T-DNA
115(2)
8.2.3 Gene of Interest
117(1)
8.3 Co-Resident Ti- and At-Plasmid Vectors
118(1)
8.4 Gene Transfer and Expression
118(1)
8.5 Infection and Co-Cultivation for Efficient Transgenesis
119(1)
8.6 Molecular Role of Organic Supplements
120(1)
8.7 DNA Repair for Efficient Integration
121(1)
8.8 Immunity to Plant Transformation
122(1)
8.9 Summary
123(1)
8.10 Abbreviations
123(4)
References
124(3)
Chapter 9 Genetic Transformation in Other Leguminous Crops
127(14)
9.1 Introduction
127(1)
9.2 Status of Genetic Transformation in Other Legume Crops
128(8)
9.2.1 Chickpea -- Cicer arietinum
129(1)
9.2.2 Pigeon Pea -- Cajanus cajan
130(1)
9.2.3 Lentil -- Lens culinaris
130(2)
9.2.4 Common Bean -- Phaseolus vulgaris
132(1)
9.2.5 Peas -- Pisum sativum
133(1)
9.2.6 Faba Bean -- Vicia faba
133(1)
9.2.7 Cowpea -- Vigna unguiculata
134(1)
9.2.8 Mung Bean -- Vigna radiata
135(1)
9.3 Summary
136(1)
9.4 Abbreviations
137(4)
References
137(4)
Chapter 10 Transgenic Plants for Biotic Stress Resistance in Soybean
141(10)
10.1 Introduction
141(1)
10.2 Defining Biotic Stress
141(3)
10.3 Plant-Pathogen Interactions
144(1)
10.4 Soybean-Pathogen Specificity Genes
145(1)
10.5 Soybean Defence against Biotic Stress
146(1)
10.6 Transgenics Conferring Biotic Stress Resistance
147(1)
10.7 Summary
148(1)
10.8 Abbreviations
148(3)
References
149(2)
Chapter 11 Transgenic Plants for Abiotic Stress Resistance in Soybean
151(12)
11.1 Introduction
151(1)
11.2 Defining Abiotic Stress
152(1)
11.3 Abiotic Stress Impact on Soybean Plants
152(3)
11.3.1 Salinity
153(1)
11.3.2 Heat Stress
154(1)
11.3.3 Chilling Stress
154(1)
11.3.4 Drought Stress
155(1)
11.4 General Defence
155(2)
11.5 Resistance Genes for Abiotic Stress Tolerance
157(1)
11.6 Abiotic Stress-Tolerant Varieties
158(1)
11.7 Summary
159(1)
11.8 Abbreviations
160(3)
References
160(3)
Chapter 12 Potential Health Safety Concerns and Environmental Risks
163(18)
12.1 Introduction
163(3)
12.2 Evidence of Biosafety and Risks Involving Agrobacterium tumefaciens
166(1)
12.3 Unintended T-DNA and Selectable Markers Transfer
167(1)
12.4 Gene Transfer to Non-Targeted Hosts
168(1)
12.5 Undesirable Gene Flow to Closely Related Species
169(1)
12.6 Potential Risks to Human and Animal Health
170(1)
12.7 Environmental Risk Assessment and Contamination
171(2)
12.8 Microbial Competition with Transformed Agrobacterium Strains
173(1)
12.9 Impact of Agrobacterium on the Environment
174(1)
12.10 Summary
174(1)
12.11 Abbreviations
175(6)
References
175(6)
Chapter 13 The Regulatory Management of Transgenic Plants
181(8)
13.1 Introduction
181(1)
13.2 Global Approach to GMO Regulations
182(1)
13.3 Risk Analysis and Management
183(1)
13.3.1 Risk Assessment of GM-Crops
183(1)
13.3.2 Risk Communication of GM-Crop Analysis
183(1)
13.3.3 Risk Management of Potential Transgenic Hazards
184(1)
13.4 Regulation and Sanctions of Transgenic Products
184(2)
13.5 Summary
186(1)
13.6 Abbreviations
187(2)
References
187(2)
Chapter 14 Current and Future Prospects in Genetic Engineering
189(14)
14.1 Introduction
189(1)
14.2 Current Challenges in Plant Transformation
189(2)
14.3 Role of GM-Crops in Agriculture
191(1)
14.4 The Importance of GMOs for Food Security
192(1)
14.5 Other Plant Breeding Methods
193(2)
14.5.1 Mutation Breeding and Plant Mutagenesis
193(1)
14.5.2 Microprojectile Bombardment
194(1)
14.5.3 Genomic Editing
195(1)
14.6 Improvement of Crop Traits
195(1)
14.7 Development of Stress-Resistant Crops
196(1)
14.8 Legislation and Social Acceptance
197(2)
14.9 Future Prospects
199(1)
14.10 Summary
199(1)
14.11 Abbreviations
200(3)
References
201(2)
Chapter 15 Photographic Index of Soybean Transformation Cultures
203(12)
15.1 Introduction
203(1)
15.2 The Soybean Plant
203(2)
15.3 Seed Sterilisation and Germination
205(1)
15.4 Cotyledonary Node Explants
205(2)
15.5 Agrobacterium tumefaciens and Co-Cultivation of Explants
207(1)
15.6 Shoot Induction
207(2)
15.6.1 In Vitro Elongation and Rooting
207(2)
15.7 Plant Hardening and Acclimatisation
209(1)
15.8 Green Fluorescent Protein (GFP)
210(1)
15.9 Summary
210(2)
15.10 Abbreviations
212(3)
References
212(3)
Glossary 215(6)
Index 221
Phetole Mangena obtained his PhD degree in Botany in the field of plant physiology and biotechnology. He is a senior lecturer and researcher in the Department of Biodiversity, School of Molecular and Life Sciences, Faculty of Science and Agriculture at the University of Limpopo, Republic of South Africa. Dr Mangenas work involve micropropagation via tissue culture, crop improvement and breeding through mutagenic chemicals as well as Agrobacterium-mediated genetic transformation. He has authored and co-authored numerous scientific papers and book chapters related to genetic transformation and morpho-physiological responses of plants to stress. Dr. Mangena is the sole editor of the Book: Advances in Legume Research- Physiological Responses and Genetic Improvement for Stress Resistance, published in 2020 (Volume 1) by Bentham Science Publishers Pte. Ltd, Singapore. He currently serves as the editor for the Scopus indexed Research Journal of BioTechnology and a fellow-member of the World Researchers Association. He is also a board member of the New Plant Biotechnology Persa Journal, member of the South African Association of Botanists and the International Society for Horticultural Science. Dr. Mangena has received numerous honours and awards, including 1st prize in the Innovation Hub GAP Bioscience Competition and the Research Excellence Award by the National Research Foundation of South Africa.
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