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ix | |
Foreword |
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xiii | |
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Preface |
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xv | |
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Section 1 Starting a New Laboratory and Training Protocols |
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1 Establishing and Equipping a New IVF Laboratory |
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1 | (7) |
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2 Basic Embryology Skills in the IVF Laboratory |
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8 | (7) |
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3 Sperm Preparation for IVF: Training Protocol |
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15 | (5) |
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4 Oocyte Pickup for IVF: Training Protocol |
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20 | (9) |
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5 Embryo Transfer for IVF: Training Protocol |
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29 | (7) |
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6 ICSI for IVF: Training Protocol |
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36 | (8) |
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7 Embryo Assisted Hatching for IVF: Training Protocol and Method |
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44 | (9) |
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8 Embryo Biopsy for IVF: Training Protocol |
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53 | (10) |
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9 Vitrification for IVF: Training Protocol |
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63 | (6) |
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10 Mouse Embryo Assay for Quality Control in the IVF Laboratory |
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69 | (4) |
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11 Sperm Survival Assay for Quality Control in the IVF Laboratory |
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73 | (6) |
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Section 2 Pre-Procedure Protocols |
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12 Quality Control in the IVF Laboratory: Continuous Improvement |
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79 | (9) |
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13 Setup Procedures for Optimizing Performance in the IVF Laboratory |
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88 | (9) |
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14 Sperm Preparation for Therapeutic IVF |
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97 | (9) |
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15 Processing Surgically Retrieved Sperm in the IVF Laboratory |
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106 | (6) |
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16 Cryopreservation of Sperm for IVF: Semen Samples and Individual Sperm |
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112 | (7) |
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17 Oocyte Collection for IVF |
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119 | (6) |
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18 In Vitro Maturation of Oocytes for IVF |
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125 | (7) |
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19 Oocyte Grading by Morphological Evaluation |
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132 | (6) |
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20 Vitrification of Oocytes for IVF |
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138 | (7) |
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Section 4 Insemination/ICSI |
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145 | (7) |
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22 Conventional IVF with Short Co-Incubation |
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152 | (4) |
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23 Technical Aspect of ICSI for Ejaculated Spermatozoa |
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156 | (7) |
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24 Sperm Selection for ICSI by Morphology |
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163 | (9) |
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25 Sperm Selection for ICSI by Viability |
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172 | (4) |
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26 Artificial Oocyte Activation for IVF |
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176 | (5) |
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Section 5 Fertilization Assessment |
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27 Fertilization Assessment in IVF and ICSI |
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181 | (6) |
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28 Polar Body Biopsy for IVF |
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187 | (5) |
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29 Zygote Cryopreservation for IVF |
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192 | (9) |
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Section 6 Embryo Assessment |
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30 Embryo Assessment at the Pre-Compaction Stage in the IVF Laboratory |
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201 | (6) |
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31 Embryo Assessment at the Post-Compaction Stage in the IVF Laboratory |
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207 | (7) |
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32 Embryo Assessment at the Blastocyst Stage in the IVF Laboratory |
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214 | (7) |
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33 Embryo Culture and Assessment via Time-Lapse Microscopy in the IVF Laboratory |
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221 | (6) |
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Section 7 Embryo Cryopreservation |
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34 Cryopreservation of Pre-Compaction Embryos for IVF |
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227 | (7) |
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35 Cryopreservation of Post-Compaction Embryos for IVF |
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234 | (11) |
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36 Embryo Biopsy by Laser on Day 5 in the IVF Laboratory |
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245 | (8) |
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Section 9 Embryo Transfer |
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37 Routine Embryo Transfer in the IVF Laboratory |
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253 | (6) |
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Section 10 Quality Management |
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38 Quality Management in the IVF Laboratory: Witnessing |
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259 | (7) |
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39 Quality Management in the IVF Laboratory: Quality Improvement, Document and Process Control and Adverse Events |
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266 | (11) |
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40 Troubleshooting in the IVF Laboratory |
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277 | (10) |
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Index |
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287 | |