Primed in situ labeling (PRINS) and in situ polymerase chain reaction (PCR) techniques have become for studying chromosomal aneuploidy and rearrangements, gene localization and expression, and genomic expression. Pellestor (Institute of Human Genetics, Centre National de la Recherche Scientifique, France) presents a completely revised and updated edition of a work describing laboratory procedures in the field, from basic methodology to research and clinical applications. Among the protocols detailed in the 21 chapters are detection and sizing of telomeric and other simple repeats by dideoxy-PRINS, brain tissue preparations for chromosomal PRINS labeling, dual-color PRINS for in situ detection of fetal cells in maternal blood, and application of reverse transcription in situ PCR in cancer analysis. The protocols offer step-by-step laboratory instruction, discussion of the principles behind the technique, lists of equipment and reagents, and tips on troubleshooting and pitfalls. Annotation ©2006 Book News, Inc., Portland, OR (booknews.com)
The in situ hybridization and PCR technologies are now well-established molecular techniques for studying chromosomal aneuploidy and rearran- ments, gene localization and expression, and genomic organization. Over the last decade, we have seen increasing applications in these fields. By combining the high sensitivity of the PCR reaction and the cytological localization of target sequences, both PRINS and in situ PCR techniques have provided highly powerful complements to FISH for in situ cellular and molecular investigations. Both these approaches have several advantages in terms of sensitivity and specificity, owing to the use of primers and to the fast kinetics of annealing and elongation reactions in situ. In the first edition of PRINS and In Situ PCR Protocols edited by John R. Gosden, experts in the field presented in detail a variety of applications of PRINS and in situ PCR techniques, in a wide range of clinical conditions. Since the publication of this successful reference book, there have been s- nificant improvements in in situ detection techniques. This completely revised and updated second edition presents a compreh- sive selection of new procedures developed in the field of PRINS and in situ PCR technologies. The book has two sections. Part I, Basic Methodology, contains chapters that provide useful protocols for many variations of PRINS and in situ PCR, including a new fast multicolor PRINS method, and protocols for PRINS detection of unique sequences in situ.
