Protein Electrophoresis: Methods and Protocols Softcover reprint of the original 1st ed. 2012 [Minkštas viršelis]

How It All Began -- A Personal History of Gel-Electrophoresis.-
Introduction to Protein Electrophoresis.- Protein Quantification Methods to
Determine Protein Concentration Prior to Electrophoresis.- Protein
Solubilization: Attend to the Choice of Lysis Buffer.- Native Polyacrylamide
Gels.- Cationic Electrophoresis.- SARCOSYL-PAGE: A New Electrophoretic Method
for the Separation and Immunological Detection of PEGylated Proteins.-
Tricine-SDS-PAGE.- Analysis of Protein Glycation using Phenylboronate
Acrylamide Gel Electrophoresis.- Protein Electrophoresis in Agarose Gels for
Separating High Molecular Weight Proteins.- Increase in Local Protein
Concentration by Field-Inversion Gel Electrophoresis.- A Combined Free Flow
Electrophoresis and DIGE Approach to Compare Proteins in Complex Biological
Samples.- Immunofixation Electrophoresis for Identification of Proteins and
Specific Antibodies.- Microchip Electrophoresis, with respect to Profiling
of A Peptides in the Cerebrospinal Fluid of Patients with Alzheimers
Disease.- A Foodomics Approach: CE-MS for Comparative Metabolomics of Colon
Cancer Cells Treated with Dietary Polyphenols.- Peptide Fractionation by
SDS-Free Polyacrylamide Gel Electrophoresis for Proteomic Analysis via
DF-PAGE.- Tris-Acetate Polyacrylamide Gradient Gels for the Simultaneous
Electrophoretic Analysis of Proteins of Very High and Low Molecular Mass.-
SDS PAGE for 35S Immunoprecipitation and Immunoprecipitation
WesternBlotting.- Two Dimensional Gel Electrophoresis: Vertical Isoelectric
Focusing.- Isoelectric Focusing in Agarose Gel for Detection of Oligoclonal
Bands in Cerebrospinal and Other Biological Fluids.- Native Flat-Bed
Isoelectric Focusing for Determining Antibody Clonotype Distribution.-
Two-Dimensional Gel Electrophoresis: Glass Tube-Based IEF Followed by
SDS-PAGE.- One-Dimensional and Two-Dimensional Immobilized Metal Affinity
Electrophoresis.- Two-Dimensional Difference Gel Electrophoresis.- Protein
Identification on Archived 2-D Gels.- Diagonal Electrophoresis for the
Detection of Protein Disulfides.- Two-Dimensional Blue Native/SDS Gel
Electrophoresis of Multiprotein Complexes.- Immunoelectrophoresis: A Method
with Many Faces.- Cell Surface Protein Biotinylation for SDS PAGE Analysis.-
A Multichannel Gel Electrophoresis and Continuous Fraction Collection
Apparatus for High-Throughput Protein Separation and Characterization.- Gel
Absorption-Based Sample Preparation Method for Shotgun Analysis of Membrane
Proteome.- Interactions of Hemoglobin in Live Red Blood Cells Measured by the
Electrophoresis Release Test.- Extraction of Proteins from Gels - A Brief
Review.- A New Algorithm for the Simulation of SDS 2D-PAGE Datasets.-
Isolation of  Proteins from Polyacrylamide Gels.- Gel Drying Methods.-
Long-Term, Buffer-Less Wet Gel Storage in Non-Sealed Polyethylene Bags.- A
Brief Review of Other NotableElectrophoretic Methods.- Protein Stains and
Applications.- Coomassie-Brilliant Blue Staining of Polyacrylamide Gels.-
Accelerated Coomassie Blue Protein Staining and Destaining with Application
of Heat.- Silver Staining Techniques of Polyacrylamide Gels.- Revisit of
Imidazole-Zinc Reverse Stain for Protein Polyacrylamide Gel Electrophoresis.-
Counterion Dye Staining of Proteins in One- and Two-Dimensional Sodium
Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis and Tryptic Gel Digestion
of Stained Protein for Mass Spectrometry.- TEMED Enhanced Photoluminescent
Imaging of Human Serum Proteins by Quantum Dots after PAGE.- Microwave
Assisted Protein Staining, De-Staining and In-Gel / In-Solution Digestion of
Proteins.- Electrophoretic Separation and Detection of Metalloproteins by
X-ray Fluorescence Mapping.- Fluorescent Staining of Gels.- A Single-Step
Simultaneous Protein Staining Procedure for Polyacrylamide Gels and
Nitrocellulose Membranes by Alta During Western Blot Analysis.- Detection of
Glycoproteins in Polyacrylamide Gels Using Pro-Q Emerald 300 Dye, a
Fluorescent Periodate Schiff-Base Stain.- Spicy SDS-PAGE Gels:
Curcumin/Turmeric as an Environment-Friendly Protein Stain.- In-Gel Staining
of Proteins in Native Poly Acryl Amide Gel  Electrophoresis Using
meso-Tetrakis(4-sulfonato phenyl)porphyrin.- Rapid Detection of Proteins in
Polyacrylamide Electrophoresis Gels with Direct Red 81 and Amido Black.-
Zymographic Assay of Oxidases Using Peroxidase or HeminEntrapped in
Polyacrylamide Gels.- Coomassie Brilliant Blue Removal/Disposal from Gel
Destain and Used Gel Stain in an Environment-Friendly Manner.- A Brief Review
of Other Notable Protein Detection Methods on Acrylamide Gels.-
Ultra-Sensitive Protein Detection and Imaging: Comparison of Lumitein,
ProteoSilver, SYPRO® Ruby, and Coomassie® Brilliant Blue Gel Stains.- Common
Artifacts and Mistakes Made in Electrophoresis.