El. knyga: Protein-Ligand Interactions: Methods and Applications

Assessing and Improving Protein Sample Quality.- A Familiar
Protein-Ligand Interaction Revisited with Multiple Methods.- Interactions of
a Signal Transduction Protein Investigated by Fluorescence Stopped-Flow
Kinetics.- Kinetic Methods of Deducing Binding Mechanisms Involving
Intrinsically Disordered Proteins.- Isothermal Titration
Calorimetry.- Measuring the Kd of Protein-Ligand Interactions Using
Microscale Thermophoresis.- Quartz Crystal Microbalance with Dissipation
Monitoring (QCM-D); Preparing Functionalized Lipid Layers for the Study of
Complex Protein-Ligand Interactions.- Indirect Detection of Ligand Binding by
Thermal Melt Analysis.- The Use of Acoustic Mist Ionization Mass Spectrometry
for High-throughput Screening.- Ligand Discovery High Through-put Binding:
Fluorescence Polarization (Anisotropy).- Fragment Screening by NMR.- A Quick
Primer in Fluorescence-based Equilibrium and Pre-Steady State Methods for
Determining Protein-Nucleotide Affinities.- Measurement of Nucleotide
Hydrolysis using Fluorescent Biosensors for Phosphate.- Gel-based Analysis of
Protein-Nucleic Acid Interactions.- Biophysical Studies of the Binding of
Viral RNA with the 80S Ribosome using SwitchSENSE.- Biolayer Interferometry:
Protein-RNA Interactions.- Analysis of Protein-DNA Interactions Using Surface
Plasmon Resonance and a ReDCaT Chip.- Characterization of Protein Nucleic
Acid complexes by Size-Exclusion Chromatography Coupled with Light
Scattering, Absorbance and Refractive Index Detectors.- Analytical
Ultracentrifugation for Analysis of Protein Nucleic Acid
Interactions.- Studying RNA-Protein Complexes Using X-ray
Crystallography.- Flow Linear Dichroism of Protein-membrane Systems.- Probing
Protein-Membrane Interactions and Dynamics Using Hydrogen-Deuterium eXchange
Mass Spectrometry (HDX-MS).