Radiation Oncology and Radiotherapy, Part A, Volume 172 in the Methods in Cell Biology series, highlights advances in the field, with this new volume presenting interesting chapters on timely topics, including DNA damage quantification by the COMET assay, Immunofluorescence microscopy-assisted quantification of ATM and ATR activation in irradiated cells, Immunoblotting-based characterization of the DNA damage response, Assessment of lipid peroxidation in irradiated cells, A simple method to assess clonogenic survival of irradiated cancer cells, Quantification of beta-galactosidase activity as a marker of radiation-driven cellular senescence, Cytofluorometric assessment of cell cycle progression in irradiated cells, and more.
Other sections cover Assessment of transcription inhibition as a characteristic of immunogenic cell death, Assessment of eIF2a phosphorylation during immunogenic cell death, Quantification of cytosolic DNA species by immunofluorescence and automated image analysis, Flow cytometry-assisted quantification of CALR exposure during immunogenic cell death, Interference of immunogenic anticancer therapy by artificially controlled calreticulin secretion from tumor cells, along with many additional topics of interest.
- Provides the authority and expertise of leading contributors from an international board of authors
- Presents the latest release in the Methods in Cell Biology series
- Includes the latest information on the topic of development, characterization and applications in CAR T Cells
1. DNA damage quantification by the COMET assay
Mathias Weyland
2. Immunofluorescence microscopy-assisted quantification of ATM and ATR
activation in irradiated cells
Ilio Vitale
3. Immunoblotting-based characterization of the DNA damage response
Ilio Vitale
4. Assessment of lipid peroxidation in irradiated cells
Boyi Gan, Chao Mao, Amber Horbath and Guang Lei
5. A simple method to assess clonogenic survival of irradiated cancer cells
Maria Esperanza Rodriguez Ruiz
6. Quantification of beta-galactosidase activity as a marker of
radiation-driven cellular senescence
Giulia Petroni
7. Cytofluorometric assessment of cell cycle progression in irradiated cells
Giulia Petroni, Ai Sato, Aitziber Buqué Martķnez and Lorenzo Galluzzi
8. Assessment of transcription inhibition as a characteristic of immunogenic
cell death
Guido Kroemer, Oliver Kepp, Giulia Cerrato and Allan Sauvat
9. Assessment of eIF2 phosphorylation during immunogenic cell death
Guido Kroemer, Oliver Kepp and Lucillia Bezu
10. Quantification of cytosolic DNA species by immunofluorescence and
automated image analysis
Lorenzo Galluzzi
11. Flow cytometry-assisted quantification of CALR exposure during
immunogenic cell death
Jitka Fucikova
12. Interference of immunogenic anticancer therapy by artificially controlled
calreticulin secretion from tumor cells
Guido Kroemer, Oliver Kepp, Peng LIU and Liwei Zhao
13. Assessment of type I interferon responses as a feature of immunogenic
cell death.
Guido Kroemer, Oliver Kepp, Sabrina Forveille, Allan Sauvat and Liwei Zhao
14. Assessment of immunological memory formation in vivo
Guido Kroemer, Oliver Kepp, Liwei Zhao and Peng LIU
15. Characterization of the SASP of cancer cells experiencing DNA damage
Francis Rodier
16. ELISA-based quantification of type I IFN secretion by irradiated cancer
cells
Claire I. Vanpouille-Box
17. RT-PCR-assisted quantification of type I IFN responses by irradiated
cancer cells
Lorenzo Galluzzi
18. Methods to characterize the exosomal output of irradiated cancer cells
Sheila Spada
19. Cytofluorometric assessment of acute cell death responses driven by
radiation therapy
Lorenzo Galluzzi
Ai Sato is a research specialist at Stich Radiation Oncology, New York, NY, USA Jeffrey Kraynak is a research laboratory specialist at Stich Radiation Oncology, New York, NY, USA. Dr. Ariel E. Marciscano is an assistant professor of Radiation Oncology at Weill Cornell Medical College. Lorenzo Galluzzi is Assistant Professor of Cell Biology in Radiation Oncology at the Department of Radiation Oncology of the Weill Cornell Medical College, Honorary Assistant Professor Adjunct with the Department of Dermatology of the Yale School of Medicine, Honorary Associate Professor with the Faculty of Medicine of the University of Paris, and Faculty Member with the Graduate School of Biomedical Sciences and Biotechnology of the University of Ferrara, the Graduate School of Pharmacological Sciences of the University of Padova, and the Graduate School of Network Oncology and Precision Medicine of the University of Rome La Sapienza. Moreover, he is Associate Director of the European Academy for Tumor Immunology and Founding Member of the European Research Institute for Integrated Cellular Pathology.
Galluzzi is best known for major experimental and conceptual contributions to the fields of cell death, autophagy, tumor metabolism and tumor immunology. He has published over 450 articles in international peer-reviewed journals and is the Editor-in-Chief of four journals: OncoImmunology (which he co-founded in 2011), International Review of Cell and Molecular Biology, Methods in Cell biology, and Molecular and Cellular Oncology (which he co-founded in 2013). Additionally, he serves as Founding Editor for Microbial Cell and Cell Stress, and Associate Editor for Cell Death and Disease, Pharmacological Research and iScience.
