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El. knyga: Reactive Oxygen Species in Plants: Methods and Protocols

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  • Formatas: EPUB+DRM
  • Serija: Methods in Molecular Biology 2526
  • Išleidimo metai: 03-Jun-2022
  • Leidėjas: Springer-Verlag New York Inc.
  • Kalba: eng
  • ISBN-13: 9781071624692
Kitos knygos pagal šią temą:
Reactive Oxygen Species in Plants: Methods and ProtocolsDidesnis paveikslėlis
  • Formatas: EPUB+DRM
  • Serija: Methods in Molecular Biology 2526
  • Išleidimo metai: 03-Jun-2022
  • Leidėjas: Springer-Verlag New York Inc.
  • Kalba: eng
  • ISBN-13: 9781071624692
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This detailed volume explores techniques to study reactive oxygen species (ROS) in plants and to characterize their roles in development and stress responses. Beginning with a section on strategies to induce ROS production, the book continues with methods to visualize ROS and detect changes in redox homeostasis, small-scale and targeted analyses for investigating the effects of ROS accumulation during stress on plant physiology and metabolism, as well as systems biology approaches to understand ROS functions. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Reactive Oxygen Species in Plants: Methods and Protocols serves as a vital resource that any researcher, and in particular young researchers, can use and adapt to further our knowledge of this dynamic area of plant science.
Modification of Chloroplast Antioxidant Capacity by Plastid
Transformation.- Analysis of Ascorbate Metabolism in Arabidopsis under
High-Light-Stress.- Genetic Manipulation of Reactive Oxygen Species (ROS)
Homeostasis Utilizing CRISPR/Cas9-Based Gene Editing in Rice.- Studying Plant
Stress Reactions In Vivo by PAM Chlorophyll Fluorescence Imaging.- Live
Monitoring of ROS-Induced Cytosolic Redox Changes with roGFP2-Based Sensors
in Plants.- Quantitative Measurement of Ascorbate and Glutathione by
Spectrophotometry.- Measurement of NAD(P)H and NADPH-Generating
Enzymes.- Quantitative Analysis for ROS-Producing Activity and Regulation of
Plant NADPH Oxidases in HEK293T Cells.- Estimation of the Level of Abasic
Sites in Plant mRNA Using Aldehyde Reactive Probe.- A Simplified Method to
Assay Protein Carbonylation by Spectrophotometry.- In Vitro Biochemical
Analysis of Recombinant Plant Proteins under Oxidation.- Methods to Analyze
the Redox Reactivity of Plant Proteins.- Determination of ROS-Induced Lipid
Peroxidation by HPLC-Based Quantification of Hydroxy Polyunsaturated Fatty
Acids.- Detection of Lipid Peroxidation-Derived Free Azelaic Acid, a Biotic
Stress Marker and Other Dicarboxylic Acids in Tobacco by Reversed Phase
HPLC-MS under Non-Derivatized Conditions.- Determination of Reactive Carbonyl
Species, which Mediate Reactive Oxygen Species Signals in Plant Cells.-
Measuring Stress-Induced Changes in Defense Phytohormones and Related
Compounds.- Targeted Mass Spectrometry Analysis of Protein Phosphorylation by
Selected Ion Monitoring Coupled to Parallel Reaction Monitoring (tSIM/PRM).-
Quantitative Analysis of Post-Translational Modifications of Plant Histones.-
Characterization of RBPome in Oxidative Stress Conditions.- Analysis of
ROS-Triggered Changes in the Transcriptome.
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