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El. knyga: Retinoids: Methods and Protocols

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  • Formatas: PDF+DRM
  • Serija: Methods in Molecular Biology 652
  • Išleidimo metai: 16-Jun-2010
  • Leidėjas: Humana Press Inc.
  • Kalba: eng
  • ISBN-13: 9781603273251
Kitos knygos pagal šią temą:
Retinoids: Methods and ProtocolsDidesnis paveikslėlis
  • Formatas: PDF+DRM
  • Serija: Methods in Molecular Biology 652
  • Išleidimo metai: 16-Jun-2010
  • Leidėjas: Humana Press Inc.
  • Kalba: eng
  • ISBN-13: 9781603273251
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New and exciting biological functions are still being discovered for vitamin A derivatives, including the vast number of physiological activities of retinoids. In Retinoids: Methods and Protocols, expert researchers in the field present the most recent technical tools with diverse techniques for both in vitro and in vivo studies. Combining biochemical, biophysical, and cell biological techniques, the book addresses topics such as the detection and quantitation of retinoids using HPLC, mass spectrometry, and fluorescence, fluorescence anisotropy of retinol binding protein, cell culture models for studying retinoid transport and the role of retinol in embryonic stem cell culture, as well as many other detailed procedures. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective subjects, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes highlighting tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Retinoids: Methods and Protocols seeks to aid beginning and experienced researchers from widely varied fields in the search to uncover even more vital aspects of vitamin As impact on the human body.

Recenzijos

From the reviews:

This excellent book provides authoritative and cutting-edge information in the field of retinoids . It is intended both for beginning and experienced researchers in diverse fields who are interested in learning new techniques. Even graduate students, nutritionists, and clinicians could find this book informative and helpful. By combining biochemical, biophysical, and cell biological techniques, the book thoroughly addresses the field of retinoid research. (Omer Iqbal, Doodys Review Service, October, 2010)

Quantification of Endogenous Retinoids.- Culture of Highly
Differentiated Human Retinal Pigment Epithelium for Analysis of the Polarized
Uptake, Processing, and Secretion of Retinoids.- Feeder-Independent Culture
of Mouse Embryonic Stem Cells Using Vitamin A/Retinol.- In Vitro Assays of
Rod and Cone Opsin Activity: Retinoid Analogs as Agonists and Inverse
Agonists.- Physiological Studies of the Interaction Between Opsin and
Chromophore in Rod and Cone Visual Pigments.- Measurement of the Mobility of
All-Trans-Retinol with Two-Photon Fluorescence Recovery After
Photobleaching.- Microfluorometric Measurement of the Formation of
All-Trans-Retinol in the Outer Segments of Single Isolated Vertebrate
Photoreceptors.- HPLC?/?MSN Analysis of Retinoids.- Binding of Retinoids to
ABCA4, the Photoreceptor ABC Transporter Associated with Stargardt Macular
Degeneration.- Fluorescence-Based Technique for Analyzing Retinoic Acid.- The
Interaction Between Retinol-Binding Protein and Transthyretin Analyzed by
Fluorescence Anisotropy.- Assay of Retinol-Binding ProteinTransthyretin
Interaction and Techniques to Identify Competing Ligands.- Molecular Biology
and Analytical Chemistry Methods Used to Probe the Retinoid Cycle.-
Visualization of Retinoid Storage and Trafficking by Two-Photon Microscopy.-
Reverse-Phase High-Performance Liquid Chromatography (HPLC) Analysis of
Retinol and Retinyl Esters in Mouse Serum and Tissues.- Detection of Retinoic
Acid Catabolism with Reporter Systems and by In Situ Hybridization for CYP26
Enzymes.- Diet in Vitamin A Research.- Experimental Approaches to the Study
of A2E, a Bisretinoid Lipofuscin Chromophore of Retinal Pigment Epithelium.-
Analysis of the Retinoid Isomerase Activities in the Retinal Pigment
Epithelium and Retina.- Techniques to StudySpecific Cell-Surface
Receptor-Mediated Cellular Vitamin A Uptake.
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