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El. knyga: Salamanders: Methods and Protocols

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  • Formatas: PDF+DRM
  • Serija: Methods in Molecular Biology 2562
  • Išleidimo metai: 22-Oct-2022
  • Leidėjas: Springer-Verlag New York Inc.
  • Kalba: eng
  • ISBN-13: 9781071626597
Kitos knygos pagal šią temą:
Salamanders: Methods and ProtocolsDidesnis paveikslėlis
  • Formatas: PDF+DRM
  • Serija: Methods in Molecular Biology 2562
  • Išleidimo metai: 22-Oct-2022
  • Leidėjas: Springer-Verlag New York Inc.
  • Kalba: eng
  • ISBN-13: 9781071626597
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This volume provides a comprehensive reference for researchers aiming to bring new techniques and approaches to their scientific research using urodeles. Chapters are authored by leaders in the field and meant to guide readers through laboratory colony husbandry, traditional molecular techniques, experimental manipulation and surgeries, bioinformatics and genomics, transgenics and lineage-tracing, and physiological and organismal techniques. In addition to laboratory methods, this volume highlights techniques developed for field studies and work with wild-caught animals. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.

 Authoritative and state-of-the-art, Methods in Salamander Research aims to be a practical guide for researchers interested in deploying new  methodologies in their lab or in the field. 


Salamanders as key models for development and regeneration
research.- Establishing a new research axolotl colony.- Health monitoring for
laboratory salamanders.- Husbandry, captive breeding and field survey of
Chinese giant salamander (Andrias davidianus).- Whole mount in situ
hybridization (WISH) for salamander embryos and larvae.- Hybridization chain
reaction fluorescence in situ hybridization (HCR-FISH) in Ambystoma mexicanum
tissue.- Ethyl cinnamate-based tissue clearing strategies.- Induction and
characterization of cellular senescence in salamanders.- Methods for studying
appendicular skeletal biology in axolotls.- Fluorescence in situ
hybridization of DNA-probes on mitotic chromosomes of the Mexican
axolotl.- The use of small molecules to dissect developmental and
regenerative processes in axolotls.- COMET Assay for detection of DNA damage
during axolotl tail regeneration.- In vivo and Ex vivo View of Newt Lens
Regeneration.- Bead implantation and delivery of exogenous growth
factors.- The Accessory Limb Model regenerative assay and its
derivatives.- Embryonic Tissue and Blastema Transplantations.- Retinoic
acid-induced limb duplications.- Isolation and characterization of peritoneal
macrophages from salamanders.- Navigation and use of custom tracks within the
axolotl genome browser.- Chromosome Conformation Capture for large
genomes.- Axolotl transgenesis via injection of I-SceI meganuclease or
Tol2 transposon system.- A practical guide for CRISPR-Cas9-induced mutations
in axolotls.- Applying a knock-in strategy to create reporter tagged knockout
alleles in axolotls.- Baculovirus production and infection in axolotls.- Cell
dissociation techniques in salamanders.- Axolotl metabolism: measuring
metabolic energy.- Artificial insemination in axolotls.- Screening
salamanders for symbionts.- Assessing leukocyte profiles of salamanders and
other amphibians: a herpetologists guide.- Best practices to promote data
utility and recycling by the salamander community.- Now that we got there,
what's next?. 



 



 



 



 
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