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El. knyga: Schistosoma mansoni: Methods and Protocols

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  • Formatas: EPUB+DRM
  • Serija: Methods in Molecular Biology 2151
  • Išleidimo metai: 25-May-2020
  • Leidėjas: Springer-Verlag New York Inc.
  • Kalba: eng
  • ISBN-13: 9781071606353
  • Formatas: EPUB+DRM
  • Serija: Methods in Molecular Biology 2151
  • Išleidimo metai: 25-May-2020
  • Leidėjas: Springer-Verlag New York Inc.
  • Kalba: eng
  • ISBN-13: 9781071606353

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This volume details protocols for studying Schistosoma mansoni.  Chapters guide readers through reviews on current drugs and drug discovery, methods to interrogate the cell biology of the worm, protocols to look at proteome level changes, prepare and work with specific proteins from S. mansoni, describes a drug screening, and how to test potential vaccine targets. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Schistosoma mansoni: Methods and Protocols aims to ensure successful results in the further study of this vital field.

Praziquantel An Enigmatic, Yet Effective Drug.- Computer-aided Drug
Design for the Identification of Novel Antischistosomal
Compounds.- Immunofluorescent Localization of Proteins in Schistosoma
mansoni.- Sensitive Fluorescence in situ hybridization on Semi-thin Sections
of Adult Schistosoma mansoni using DIG-labeled RNA Probes.- Collection of
Excretory/Secretory Products from Individual Developmental Stages of the
Blood Fluke Schistosoma mansoni.- Labeling of the Schistosoma
mansoni Tegument.- A Procedure for Analyzing the Proteomic Profile of
Schistosoma mansoni Cercariae.- Proteomic Analysis of Schistosoma mansoni
tegumental Proteins.- Measuring Histone Modifications in the Human Parasite
Schistosoma mansoni.- Step-by-Step Bioinformatics Analysis of Schistosoma
mansoni Long Non-coding RNA Sequences.- Identification of Transposable
Elements in Schistosoma mansoni.- Structural and Functional Characterization
of Schistosoma mansoni Cathepsin B1.- Characterisation of Schistosoma
mansoni dihydrofolate Reductase (DHFR).- Characterisation of Schistosoma
mansoni Glutamate-Gated Chloride Channels.- A Modified ELISA Method to
Evaluate the Interaction of Schistosoma mansoni Proteins with
Plasminogen.- Biophysical analysis of Schistosoma
mansoni septins.- Crystallographic Studies of Triosephosphate Lsomerase from
Schistosoma mansoni.- Luminescence-based, Low- and medium-throughput Assays
for Drug Screening in Schistosoma mansoni Larval Stage.- Testing of
Schistosoma mansoni Vaccine Targets.